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A kind of anti-sweet potato black spot fungus compound and its preparation method and application

A black spot fungus and compound technology, applied in the field of anti-sweet potato black spot fungus compound and its preparation, to achieve the effects of easy control, simple use and mild conditions

Active Publication Date: 2021-10-26
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on biopesticides against sweet potato black spot fungus

Method used

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  • A kind of anti-sweet potato black spot fungus compound and its preparation method and application
  • A kind of anti-sweet potato black spot fungus compound and its preparation method and application
  • A kind of anti-sweet potato black spot fungus compound and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of the compound of anti-sweet potato black spot bacterium

[0027] Extract 30 L of the fermentation broth of the strain KLBMP-Pt686 with ethyl acetate at a ratio of 1:1 for three times, combine them, concentrate and dry under reduced pressure to obtain ethyl acetate extract (9.5 g). The obtained extract was mixed with silica gel (200-300 mesh), and after mixing, it was separated on a silica gel column (450mm×55mm, 200-300 mesh) by dry method, and petroleum ether: ethyl acetate (10:1 and 1:1), ethyl acetate:methanol (1:1) and methanol were used as elution solvents for gradient elution, and a total of 60 fractions were collected. The collected components were subjected to silica gel column chromatography again, eluted with a mixture of petroleum ether: acetone (3:7), the effluent was collected at 10 mL / bottle, and the solvent was evaporated to obtain a light yellow solid, which was the target compound (31.0 mg). The compound is characterize...

Embodiment 2

[0029] Example 2: Determination of the inhibitory effect of compound 2-(1-hydroxyl-2-acetoxy)propyl-7-oxabicyclo[4.1.0]-2,4-heptadiene on sweet potato black spot fungus

[0030] Pipette 100 μL of sweet potato black spot spore suspension (1 × 10 6 cfu / mL) on a PDA plate, vertically place an Oxford cup in the center of the plate, and then slightly pressurize to make there is no gap between the Oxford cup and the surface of the medium.

[0031] The compound 2-(1-hydroxy-2-acetoxy)propyl-7-oxabicyclo[4.1.0]-2,4-heptadiene was dissolved in 20% DMSO and prepared to a concentration of 400 μg / mL, 200 μg / mL and 100 μg / mL solutions.

[0032] Add 200 μL of the above-mentioned sample solutions with different concentrations into the Oxford cup respectively (20% DMSO sterile solution is used as the control), and repeat three times. After the sample was added, the plate was placed in an incubator for 4 days, and the antibacterial results were observed and the diameter of the inhibition zo...

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Abstract

A compound identified as 2‑(1‑hydroxy‑2‑acetoxy)propyl‑7‑oxabicyclo[4.1.0]‑2,4‑heptanyl, which is useful against Black Spot of Sweetpotato Diene. The preparation method of the compound is as follows: a strain of Hypocreales sp. is activated and cultured and then inoculated on the medium for fermentation culture, and then the supernatant after the fermentation culture is extracted with ethyl acetate and concentrated to obtain Ethyl acetate extract, and finally the ethyl acetate extract was purified by column chromatography twice to obtain the target product. The experimental results of the inhibitory effect of the compound on sweet potato black spot fungus show that it has a good inhibitory effect on sweet potato black spot fungus, and can be used as a biological pesticide instead of chemical pesticides for preventing and treating sweet potato black spot disease. The compound is a natural product, has no pollution, no toxin residue, and no harm to humans, animals and the environment.

Description

technical field [0001] The invention relates to the field of biopesticides, in particular to a compound resistant to sweet potato black spot fungus and its preparation method and application. Background technique [0002] Sweet potato black spot fungus, also known as Ceratocystis fimbriata Elliset Halsted, which infects sweet potatoes and can cause sweet potato black spot, is an important disease in sweet potato production. It is widely distributed and causes serious losses to sweet potato production in my country. At present, the control technologies for sweet potato black spot mainly include agricultural control and chemical control. Agricultural control refers to the agricultural technology used to prevent and control crop diseases, that is, to change the growth environment of crops to enhance the resistance of crops to diseases. The main measures of agricultural prevention and control include three aspects: 1. Breeding, that is, to select seeds with disease resistance, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D303/16A01N43/20A01P3/00C12P17/02C12R1/645
CPCA01N43/20C07D303/16C12P17/02
Inventor 鞠秀云张健蒋继宏
Owner XUZHOU NORMAL UNIVERSITY
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