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Bacillus subtilis, screening method and applications thereof

A Bacillus subtilis, screening method technology, applied in application, botanical equipment and methods, microorganism-based methods, etc., can solve problems such as no public reports, and achieve the effect of good inhibition and harm reduction

Inactive Publication Date: 2018-08-21
YICHUN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no public report on the use of Bacillus subtilis as a degrading bacterium for the self-toxicity of strawberry continuous cropping

Method used

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  • Bacillus subtilis, screening method and applications thereof
  • Bacillus subtilis, screening method and applications thereof
  • Bacillus subtilis, screening method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the screening of degrading bacterial strain L501 and the degradation rate detection of p-benzoic acid

[0067] The standard curve of benzoic acid can be drawn according to the measured data of the standard curve of benzoic acid, and the data are shown in Table 1 below. Standard curve is the existing standard curve of benzoic acid.

[0068] Table 1 Benzoic acid standard curve determination data table

[0069] Table 1Table of standard curve for benzoic acid determination

[0070]

[0071] According to the experimental analysis, it can be seen from the following table 2 that among the 67 strains isolated and purified, 9 strains are respectively L203, L302, L403, L501, L502, R401, R402, R403, and R503, all of which have the ability to degrade benzoic acid . The degradation rates were 13.3%, 17.1%, 43.2%, 87.5%, 40.8%, 49.5%, 46.1%, 57.3%, 42.7% at 168h, and L501 reached the highest at 168h, which was 87.5%. It shows that the degradation effect of Bacill...

Embodiment 2

[0076] Embodiment 2, identification of bacterial strain Bacillus subtilis L501 of the present invention

[0077] The Bacillus subtilis L501 of the present invention has the highest degradation rate of benzoic acid at 168 hours, and the degradation rate is 87.5%. It is therefore identified. Morphological observation showed that the colonies of degrading bacteria were milky white to beige, round, with smooth and moist edges, slightly denticulate, wrinkled on the surface, raised in the middle, and opaque in texture. Under the optical microscope, it can be observed that the bacterial strain is a Gram-positive bacterium, which is a short bacillus, with a size of (0.7-0.9) μm×(2.0-3.0) μm; the spores are slightly enlarged ( figure 1 ).

[0078] Molecular identification The 16S rDNA sequence of strain L501 is 1456bp in length, submitted to GenBank, and obtained the sequence number as MG519283, using the BLAST program on the NCBI website to conduct a comparative analysis of the sequ...

Embodiment 3

[0081] Embodiment 3, the in-dish antagonism of degrading bacteria of the present invention to strawberry root rot

[0082] The fermentation broth of the degrading strain L501 showed antagonistic activity, and the results are shown in Table 3. Among them, it has a strong inhibitory effect on the growth of the F405Fusarium solani strain, the diameter of the antagonistic circle is 15.0mm, and the antagonistic diameter of F403Fusarium. very good. In summary, the degrading strain L501 has a good inhibitory effect on strawberry root rot.

[0083] Table 3. Diameters of antagonistic circles of L501 against three strawberry root rot pathogens

[0084] Tab.3Diameters of the inhibitory zones of strain L501against threestrawberry root rot

[0085]

[0086] The above research of the present invention shows that Bacillus subtilis L501 of the present invention is a biocontrol bacterium, and has been initially developed into a relevant biocontrol agent and put into the market for testin...

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Abstract

The invention provides a strain of Bacillus subtilis, a screening method and applications thereof, wherein the Bacillus subtilis is preserved in the China Center for Type Culture Collection, has the preservation number of CCTCC No:M 2017729, has the classification name of Bacillus subtilis L501, is mainly used for the degradation of strawberry succession cropping obstacle induced autotoxicity substance benzoic acid, wherein the benzoic acid degradation rate is up to 87.5%. According to the present invention, with the Bacillus subtilis, benzoic acid in the rhizosphere of strawberry is effectively degraded, the main strawberry succession cropping obstacle is overcome, and the technical method is provided for the effective utilization of bacteria to treat strawberry succession cropping obstacle in strawberry planting production in the future so as to achieve the large-scale production of strawberry, increase the yield of strawberry, provide the technical support for the effective use of bacteria to treat strawberry succession cropping obstacle in strawberry planting production, and provide broad market prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of the Bacillus subtilis to degrade the autotoxicity obstacle substance benzoic acid in continuous cropping of strawberries, and in particular to a Bacillus subtilis and its screening method and application. Background technique: [0002] Strawberry (English name: Strawberry, scientific name: Fragaria ananassa Duch), a plant of the genus Strawberry in the Rosaceae family, is known as the queen of fruits. Its advantages such as short cultivation period, quick results, high economic benefits, and suitable for facility cultivation have become a hotspot for agricultural planting in various countries. At present, there are 62 countries producing strawberries in the world. The total annual production of strawberries in the world has exceeded 3 million tons, and the cultivation area has exceeded 200,000 ha. 2 . Strawberry planting in my country has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A62D3/02A01N63/02A01P3/00C12R1/125
CPCA62D3/02A62D2101/28A01N63/10C12N1/02C12N1/20C12N1/205C12R2001/125
Inventor 刘小林刘紫英黄磊沈春修却志群禹超颖
Owner YICHUN UNIVERSITY
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