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Application of daphnetin in preparation of drugs to treat non-alcoholic fatty liver disease

A non-alcoholic, daphnetin technology, applied in drug combinations, active ingredients of heterocyclic compounds, digestive system, etc.

Inactive Publication Date: 2018-08-24
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of liver-protecting drugs can only play an adjuvant role, and it is mainly for the pathological type of NASH

Method used

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  • Application of daphnetin in preparation of drugs to treat non-alcoholic fatty liver disease
  • Application of daphnetin in preparation of drugs to treat non-alcoholic fatty liver disease
  • Application of daphnetin in preparation of drugs to treat non-alcoholic fatty liver disease

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Experimental program
Comparison scheme
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Embodiment Construction

[0020] The present invention will be described in detail below by way of examples.

[0021] 1. Experimental method

[0022] 1.1 Cell induction administration

[0023] 1) Cell induction

[0024] HepG2 cells were digested to make a uniform cell suspension, diluted in proportion to the culture time and inoculated into 6-well plates. 2 , Placed in a 37°C constant temperature incubator for 24 hours. Discard the original DMEM complete medium, slowly add PBS buffer to wash three times, add DMEM complete medium containing 0.2% sodium oleate and 10% BSA, and cultivate in the incubator for 24 hours. Observe the cell morphology under a microscope. If black particles appear on the cell surface, the induction is successful.

[0025] 2) Drug treatment

[0026] Adherent cells were first induced for 24 hours, and treated for 24 hours after administration. Set blank group, solvent group, model group and administration group.

[0027] 1.2 Detection of cell viability (MTT method)

[0028...

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Abstract

The invention discloses application of daphnetin in the preparation of drugs to treat non-alcoholic fatty liver disease. Experimental results show that daphnetin can inhibit the expression of fat synthesis key genes SREBP-1C and PNPLA3 by up-regulating the phosphorylation level of glucolipid metabolism key factor AMPK, and can reduce fat accumulation in liver cells by inducing the expression of lipid oxidation key gene PPAR alpha. Daphnetin can promote intake of glucose by liver cells, by up-regulating the expression of PI3K and pAKT / AKT level to relieve insulin resistance. Daphnetin can relieve oxidation injury of liver cells by inhibiting the expression of the oxidation generation key gene CYP4A.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to a new application of daphnetin, in particular to a new application of daphnetin in the preparation of drugs for treating non-alcoholic fatty liver. Background technique [0002] Daphnetin (Daphnetin, DAP) chemical name is 7,8-dihydroxycoumarin (7,8-dihydroxycoumarin), the active ingredient isolated from Daphnetin plant Changbai Daphnetin, is a natural coumarin derivative . Literature shows that daphnetin has good pharmacological activities such as anti-oxidation, anti-inflammation, anti-coagulation, and anti-virus, and is mainly used clinically for the adjuvant treatment of thromboangiitis obliterans, other occlusive vascular diseases, and coronary heart disease. [0003] It has been reported in the literature that daphnetin can effectively protect the cytotoxicity, apoptosis and mitochondrial dysfunction induced by tert-butyl hydroperoxide, mainly by reducing the generation of reactive oxy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P1/16
CPCA61K31/37A61P1/16
Inventor 韩凤梅廖璐刘亚云陈勇
Owner HUBEI UNIV
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