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Gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and application method thereof

A technology of bone marrow stem cells and gelatin sponge, applied in the field of gelatin sponge materials containing autologous bone marrow stem cells, can solve problems such as low repair efficiency, and achieve the effects of simple operation, reduced leakage of inflammatory media, and less bone marrow blood consumption.

Pending Publication Date: 2018-08-28
TIANJIN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low repair efficiency in the prior art for the treatment of intervertebral disc degenerative diseases, and propose a gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc damage and its application method

Method used

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  • Gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and application method thereof
  • Gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and application method thereof
  • Gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Repairing Goat Intervertebral Disc Defects by Enriching Autologous BMSCs and Composite Gelatin Sponge

[0029] Construct the goat intervertebral disc defect model: the goat is fasted before operation, under general anesthesia, lying on the operating table on the right side, disinfected, and covered with sterile surgical drapes. According to the position of the target segment, a longitudinal incision was made 1 cm in front of the edge of the left transverse process, and the superficial fascia, deep fascia, and muscular layer were separated in sequence to the retroperitoneum. Along the retroperitoneal space to the front side of the vertebral body, separate the muscles attached to the intervertebral disc to expose the target intervertebral disc. A small incision was made with a sharp knife in the left front of the intervertebral disc, and part of the nucleus pulposus was removed with nucleus pulposus forceps.

[0030] Enrichment of autologous bone marrow blood ...

Embodiment 2

[0032] Example 2: Gelatin Sponge Loaded with Autologous Bone Marrow Blood-Enriched Cells to Repair Human Intervertebral Disc Surgical Defects

[0033] Movable intervertebral disc endoscopic nucleus pulposus removal: The patient is placed in prone position, under general anesthesia, and the position and direction of the intervertebral space of the operation segment are positioned and fluoroscopyed with a "C" arm X-ray machine before the operation. A longitudinal incision about 2.5 cm long was made beside the spinous process. Cut the subcutaneous tissue and deep fascia sharply to stop the bleeding. The channel was placed close to the spinous process to reach the lamina, and the soft tissue in the channel was cleared under direct vision to expose the lower edge of the lamina and the ligamentum flavum. Connect the intervertebral disc system (Karl Storz, Germany), use Kerrison forceps to open a window in the interlaminar space under the microscope, bite off the lower edge of the u...

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Abstract

The invention discloses a gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and an application method thereof. The gelatin sponge material containing autologous bone marrow stem cells is prepared from autologous bone marrow stem cells and gelatin sponge particles, wherein the autologous bone marrow stem cells are adsorbed onto or into thegelatin sponge particles through the physical mesh structure and surface chemisorption thereof. The application method of the gelatin sponge material containing autologous bone marrow stem cells comprises the following steps: a, extracting bone marrow blood, putting the gelatin sponge particles into a negative pressure enricher for bone growth, injecting extracted bone marrow fluid, starting a negative pressure enrichment process, and repeating an enrichment cycle; b, implanting the gelatin sponge particles on which the bone marrow stem cells are enriched obtained in the step a into nucleus pulposus defects, and meanwhile suturing fibrous ring breaches. The gelatin sponge material can be applied to animal experiments of sheep, pigs, monkeys and the like. Meanwhile, the gelatin sponge material can also be used for repairing simple nucleus pulposus, simple fibrous rings and fibrous ring-nucleus pulposus simultaneously.

Description

technical field [0001] The invention relates to biological tissue engineering technology, in particular to a gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc damage and a use method thereof. Background technique [0002] Intervertebral disc herniation is a relatively common disease. Its incidence rate is increasing year by year and it is becoming younger. It seriously affects people's health and work and life. Patients with severe symptoms often need surgical treatment, resulting in huge medical expenses and social burdens. Discectomy is the standard operation for the treatment of disc herniation, and it is also the most common operation in spinal surgery. The effective rate of pain relief and function improvement is acceptable, but 20-25% of patients have postoperative results Unsatisfactory all the time, wherein prominent recurrence is one of important reasons, and literature reports recurrence rate 5-20%. The main rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22A61L27/50A61L27/56
CPCA61L27/222A61L27/3834A61L27/3856A61L27/50A61L27/56A61L2430/38
Inventor 徐宝山杜立龙袁秋明许海委张昊
Owner TIANJIN HOSPITAL
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