A kind of metal pattern based on dna nanostructure and its preparation method and application
A nanostructure, metal pattern technology, applied in the direction of nanostructure manufacturing, specific nanostructure formation, nanotechnology, etc., can solve complex operations, inability to accurately locate or build pre-designed metal nanostructures, and inability to realize a variety of different metal nanoparticles functional issues
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Embodiment 1
[0069] The preparation of embodiment 1 palladium nanowire
[0070] (1) Preparation of one-dimensional DNA nanowires: referring to Peng Yin's method and design ideas (Nature, 2012, 485, 623-626), for example, six phosphorothioylated DNA short chains were synthesized as shown in Table 1 (* represents the corresponding Phosphorothioylation modification of nucleotides) to prepare one-dimensional DNA nanowires, mix six phosphorothioylated DNA short chains in equal proportions, add 1×TA / Mg 2+ The buffer solution (40mM Tris, 20mM acetic acid, 12.5mM magnesium acetate, pH 8.0) was mixed evenly, put into a PCR machine and slowly cooled from 95°C to 4°C, and cooled for 12h to obtain a one-dimensional DNA nanowire;
[0071] (2) In situ growth of palladium nanoparticles: 1μL, 2mM palladium acetate (Pd(OAc) 2 ) solution into 50μL, 5nM one-dimensional DNA nanowire solution, let it stand for 3h, passed through 100K ultrafiltration tube, in 1×TA / Mg 2+ Centrifuge at 3500 rpm at 4°C for 3 min...
Embodiment 2
[0075] The preparation of embodiment 2 palladium nanowires
[0076] (1) Preparation of one-dimensional DNA nanowires: refer to Peng Yin's method and design ideas (Nature, 2012, 485, 623-626), and synthesize 6 DNA short chains as shown in Table 2 for the preparation of one-dimensional DNA nanowires , extend the 3' ends of 6 short DNA chains to extend 15 A bases, mix the 6 DNA short chains containing the extended chains in equal proportions, add 1×TA / Mg 2+ The buffer solution (40mM Tris, 20mM acetic acid, 12.5mM magnesium acetate, pH 8.0) was mixed evenly, put into a PCR machine and slowly cooled from 95°C to 4°C, and cooled for 12h to obtain a one-dimensional DNA nanowire;
[0077] (2) Purification: Add the prepared one-dimensional DNA nanowire solution into a 100K ultrafiltration tube, in 1×TA / Mg 2+ In the buffer solution, centrifuge at 4500 rpm, 10°C for 5 minutes, and centrifuge twice to obtain purified one-dimensional DNA nanowires;
[0078] (3) Sulfhydryl modification: t...
Embodiment 3
[0083] Example 3 Preparation of L-type iron nanopatterns
[0084] (1) Preparation of two-dimensional rectangular DNA nanostructures: referring to Paul Rothemund's method and staple chain design ideas (Nature, 2006, 440, 297-302), the design pattern is as follows figure 2 As shown in the L-type, synthesize the staple chain shown in Table 3, and part of the staple chain (numbered as R38-R46, R51-R59, R62-R70, R82, R83, R93, R94, R106, R107, R117, R118, R130, R131, R141, R142, R154, R155, R165, R166, R178 and R179) 3' extended strands of 30 A bases, according to M13 scaffold chain: staple chain Or extension chain = 1:5 ratio to add 5nM M13 scaffold chain to staple chain at 1 × TA / Mg 2+ Mix in the buffer solution, put it into a PCR machine and slowly cool down from 95°C to 4°C, and cool down for 12 hours to obtain a two-dimensional rectangular DNA nanostructure;
[0085] (2) Purification: Add the prepared two-dimensional rectangular DNA nanostructure solution into a 100K ultraf...
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