Method for extracting morroniside, loganin and ursolic acid from fructus corni

A technology of cornus officinalis and loganin, which is applied in chemical instruments and methods, preparation of sugar derivatives, steroids, etc., can solve the problems of the destruction of morroniside and loganin, and the easy destruction of morroniside, and achieves improved extraction. efficiency, reducing production costs, and preventing hydrolysis

Inactive Publication Date: 2018-08-31
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Aiming at the problems that morroniside is easily destroyed in the process of extracting ursolic acid from Cornus officinalis, the present invention provides a method for extracting morroniside, loganin and ursolic acid from Cornus officinalis, and completely solves the problem of morroniside and loganin in the extraction process. Glycoside damage and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take the fruit of Cornus officinalis, dry and crush 100kg, add 350L of 75% ethanol aqueous solution, extract at room temperature for 2 hours, filter to obtain filtrate I and filter residue I; filtrate I is concentrated to 10L under vacuum or reduced pressure at 50°C, and filter once to obtain filtrate II and filter residue II; add 15L of water to the filter residue I, inoculate with 12% Bacillus subtilis, and ferment at 30°C for 36 hours to obtain a fermented product; add 400L of 95% ethanol to the fermented product, extract at room temperature for 2 hours, and filter to obtain filtrate III and filter residue III; The filtrate III was concentrated to 15L under vacuum or reduced pressure at 50°C, and filtrate IV and filter residue IV were obtained by ceramic membrane filtration once; the filtrate II and filtrate IV were combined and then adsorbed on an AB-8 macroporous resin column. times column volume of 30% ethanol aqueous solution for elution, and eluate I was collecte...

Embodiment 2

[0043] Take the fruit of Cornus officinalis, dry and crush 200kg, add 700L of 80% ethanol aqueous solution, extract at room temperature for 2 hours, filter to obtain filtrate I and filter residue I; filtrate I is concentrated to 20L under vacuum or reduced pressure at 50°C, and filter once to obtain filtrate II and filter residue Ⅱ; add 30L of water to the filter residue I, inoculate with 12% Bacillus subtilis, and ferment at 30°C for 36 hours to obtain a fermented product; add 800L of 95% ethanol to the fermented product, extract at room temperature for 2 hours, and filter to obtain filtrate III and filter residue III; The filtrate III was concentrated to 15L under vacuum or reduced pressure at 50°C, and filtrate IV and filter residue IV were obtained by ceramic membrane filtration once; the filtrate II and filtrate IV were combined and then adsorbed on an AB‐8 macroporous resin column, using six times the column volume 30% ethanol aqueous solution was used for elution, and th...

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Abstract

The invention relates to a method for extracting morroniside, loganin and ursolic acid from fructus corni. Fructus corni is dried and crushed, alcohol extraction, filtration, vacuum or reduced pressure concentration and filtration are performed, a filtrate is obtained and fermented with bacillus subtilis, alcohol extraction and filtration are performed, filtrates are mixed, the mixture passes an AB-8 macroporous resin column, and a purified morroniside product is obtained; filter residues are dissolved with methanol, adsorption is performed with a silica gel column, ursolic acid and loganin are obtained respectively, crystallization and recrystallization are performed once, and purified products are obtained.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to a method for extracting ursolic acid, loganin and morroniside from Cornus officinalis. Background technique [0002] The ripe and dry fruit of Cornus officinalis, after removing the pit, is the precious medicinal material potato meat. The pulp contains 16 kinds of amino acids, in addition, it contains a lot of elements necessary for the human body. In addition, it contains saponin raw sugars, polysaccharides, malic acid, tartaric acid, phenols, resins, tannins and vitamins A and C with strong physiological activity. The pulp is used as medicine, which is astringent blood tonic and tonic; it can invigorate the stomach, nourish the liver and kidney, and treat anemia, low back pain, nervous and heart weakness and other diseases. Its nature and flavor are sour and astringent, enter the liver and kidney channels. Sour and astringent, it has the effect of nourishing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/04C07J63/00
CPCC07H1/08C07H17/04C07J63/008
Inventor 罗婵
Owner 长沙爱扬医药科技有限公司
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