Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for preparing low-toxicity multi-mode luminescent carbon dots based on functional polypeptides

A functional peptide, low toxicity technology, applied in luminescent materials, chemical instruments and methods, nanocarbons, etc., can solve problems such as hidden dangers of biological safety, single luminescence behavior, etc., and achieve the effect of low toxicity and uniform size distribution

Active Publication Date: 2021-07-02
SHANDONG UNIV +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, especially the hidden dangers of biological safety and the single luminescent behavior of the common carbon dots prepared by the existing methods, the present invention uses the reduced glutathione that exists in large quantities in the organism as a carbon source, and also as a carbon source. Nitrogen source and sulfur source, in N 2 Under the atmosphere, through the high-temperature pyrolysis self-assembly method, the introduction of excess oxygen-containing groups is strictly limited, the effective doping of impurity elements is realized, and multi-mode luminescent carbon dots are prepared.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing low-toxicity multi-mode luminescent carbon dots based on functional polypeptides
  • A method for preparing low-toxicity multi-mode luminescent carbon dots based on functional polypeptides
  • A method for preparing low-toxicity multi-mode luminescent carbon dots based on functional polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Water-Soluble Carbon Dots by Pyrolysis of Functional Polypeptides

[0046] Put 500mg of reduced glutathione into a porcelain boat and distribute it evenly, move it into an atmosphere tube furnace to seal, vacuumize and fill with N 2 Repeat 10 times and finally maintain normal pressure N 2 atmosphere. The temperature was raised to 240°C at a heating rate of 5°C / min, and after 2 hours of heat preservation, it was naturally lowered to room temperature, and the carbon black pyrolysis product was transferred to a mortar and ground into a powder state. Then disperse the powder into 40-50mL deionized water and ultrasonicate for 30min, the ultrasonic power is 200W, and the frequency is 80KHz to obtain a turbid solution; the obtained turbid solution is separated by suction filtration with a filter membrane with a pore size of 0.22 μm to obtain a light yellow transparent solution; The solution was put into a 500Da dialysis bag and dialyzed for 24 hours....

Embodiment 2

[0047] Example 2 Preparation of Water-Soluble Carbon Dots by Pyrolysis of Functional Polypeptides

[0048] Put 500mg of reduced glutathione into a porcelain boat and distribute it evenly, move it into an atmosphere tube furnace to seal, vacuumize and fill with N 2 Repeat 10 times and finally maintain normal pressure N 2 atmosphere. The temperature was raised to 250°C at a heating rate of 5°C / min, and after 2 hours of heat preservation, it was naturally lowered to room temperature, and the carbon black pyrolysis product was transferred to a mortar and ground into a powder state. Then disperse the powder into 40-50mL deionized water and ultrasonicate for 30min, the ultrasonic power is 200W, and the frequency is 80KHz to obtain a turbid solution; the obtained turbid solution is separated by suction filtration with a filter membrane with a pore size of 0.22 μm to obtain a light yellow transparent solution; The solution was put into a 500Da dialysis bag and dialyzed for 24 hours....

Embodiment 3

[0049] Example 3 Preparation of Water-Soluble Carbon Dots by Pyrolysis of Functional Polypeptides

[0050] Put 500mg of reduced glutathione into a porcelain boat and distribute it evenly, move it into an atmosphere tube furnace to seal, vacuumize and fill with N 2 Repeat 10 times and finally maintain normal pressure N 2 atmosphere. The temperature was raised to 260°C at a heating rate of 5°C / min, and after 2 hours of heat preservation, it was naturally lowered to room temperature, and the carbon black pyrolysis product was transferred to a mortar and ground into a powder state. Then disperse the powder into 40-50mL deionized water and ultrasonicate for 30min, the ultrasonic power is 200W, and the frequency is 80KHz to obtain a turbid solution; the obtained turbid solution is separated by suction filtration with a filter membrane with a pore size of 0.22 μm to obtain a light yellow transparent solution; The solution was put into a 500Da dialysis bag and dialyzed for 24 hours....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for preparing low-toxicity multi-mode luminescent carbon dots based on functional polypeptides, using reduced glutathione as carbon source, nitrogen source and sulfur source, using N 2 It is prepared by pyrolysis under atmosphere, and the pyrolysis product is ground, ultrasonically dispersed in water, suction filtered, and vacuum-dried to obtain carbon dot powder. In the luminescent carbon dots prepared by this method, the impurity atoms N and S play an important role in the luminescence behavior of the carbon dots, which makes there exist both excitation-independent and excitation-dependent bands in the working band, which will greatly broaden the its scope of application. In addition, due to the widespread existence of reduced glutathione in organisms, the surface of carbon dots obtained by using reduced glutathione as a carbon source contains a large number of hydroxyl groups, carboxyl groups and other groups with strong compatibility with organisms, making them water soluble. Good performance, easy to expand biological applications.

Description

technical field [0001] The invention belongs to the field of micro-nano biotechnology, and in particular relates to a method for preparing fluorescent carbon dots with low toxicity and multi-mode emission Background technique [0002] Fluorescent carbon dots, as a new type of functional nanomaterial, have attracted widespread attention in the fields of biomedicine and materials science due to their excellent biocompatibility, water solubility, and modifiability. Compared with traditional metal chalcogenide quantum dots, it does not contain cadmium or other heavy metals, which will not cause serious health damage to biological systems. However, due to the chemical properties of ordinary carbon dots and the charge and distribution of surface functional groups, they still pose potential threats and impacts on the biological microenvironment and cell behavior during the biomarking process, such as the production of reactive oxygen species that interferes with cell growth. cycle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/184C09K11/65
CPCC09K11/65C01B32/184
Inventor 周广军黄大朋王涛孙蕾蕾高鹏孙于振
Owner SHANDONG UNIV