A kind of HPMC precipitation-assisted method for extracting limonin in lemon seeds by reverse micelles

Abstract

The invention provides a method for extracting limonin in lemon seeds by HPMC sedimentation assisted reversed micelles, and the method is used for extracting and preparing limonin in lemon grains or pieces of lemon grains. The method comprises the following steps: crushing raw materials; preparing a crude extracting solution; preparing a limonin primary extracting solution; preparing a reversed micelle extracting solution and a re-extraction water phase; performing pre-extraction on the reversed micelle extracting solution for the primary extracting solution, and separating out an upper-layerorganic phase; performing reversed extraction on the upper-layer organic phase through a reversed extraction water phase, and separating out a lower-layer water phase; hydrolyzing an acidic solution for the lower-layer water phase to prepare an acidolysis solution; and purifying the acidolysis solution to prepare limonin powder. The method for extracting limonin in lemon seeds by HPMC sedimentation assisted reversed micelles can sufficiently extract limonin in lemon peel residues, and is high in yield. The whole extracting method is simple in process, is low in cost, is beneficial for amplifying and performing industrial production; the method is simple and feasible, does not need a high-value complex apparatus, does not need professional training by a related operator, and is easily popularized.

Description

technical field

[0001] The invention relates to a method for hydroxypropyl methylcellulose (HPMC) precipitation-assisted reverse micelles to extract limonin in lemon seeds, and belongs to the field of comprehensive utilization of by-products of fruit and vegetable processing and extraction of functional substances. Background technique

[0002] Limonin (Limonin) is abundantly present in Rutaceae fruits such as citrus, lemon, pomelo, etc. It is a triterpene dilactone macromolecular compound that is electrically neutral and insoluble in water, but easily soluble in organic solvents such as chloroform, methanol, ethanol, isopropanol, acetonitrile, and acetone. Medical experiments in recent years have confirmed that limonin has strong antioxidant activity, can effectively maintain the balance of active oxygen free radicals in mammals, and at the same time inhibit the proliferation of cancer cells, delay arteriosclerosis, reduce cholesterol levels, dehumidify , analgesia, anti-i...

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