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DNA vaccine for preventing and treating periodontitis as well as preparation method thereof

A technology of DNA vaccine and gingivalin, which is applied in the field of preparation, DNA vaccine, and prevention and treatment of periodontitis, can solve problems such as difficult recovery and periodontal disease damage to periodontal tissue, and achieve low production cost, easy promotion, and method simple effect

Inactive Publication Date: 2018-09-14
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The damage caused by periodontal disease to periodontal tissue is continuous and irreversible. Once it occurs, it is difficult to achieve complete recovery with current treatment methods

Method used

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  • DNA vaccine for preventing and treating periodontitis as well as preparation method thereof
  • DNA vaccine for preventing and treating periodontitis as well as preparation method thereof
  • DNA vaccine for preventing and treating periodontitis as well as preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Construction of DNA vaccine for preventing and treating periodontitis—pVAX1-HA2-fimA

[0044] Step 1, cultivation of Porphyromonas gingivalis W83 type

[0045] The frozen Porphyromonas gingivalis type W83 (preserved by the Key Laboratory of Oral Diseases Research in Guizhou Province) was streaked and cultured on BHI blood agar culture plates (purchased from Beijing Suo Laibao Biotechnology Co., Ltd.). The conditions are 37°C anaerobic box (80%N 2 , 10%H 2 , 10% CO 2 ), protected from light for 4-7 days, observed that the black colony grew to about 1 mm, and picked the single colony grown on the plate to inoculate to the BHI liquid medium (commercial flat medium, purchased from Shanghai Hengyuan Biotechnology Co., Ltd. ), cultured in an anaerobic incubator for 1-2 days, it was observed that the liquid became turbid, and a small amount of bacteria precipitated, and the bacteria liquid was collected for subsequent experiments.

[0046] Step 2. Obtaining the ...

Embodiment 2

[0127] Example 2: Experimental Study on SD Rats Immunized by Periodontitis DNA Vaccine

[0128] 1. Mass preparation of plasmid vaccines

[0129] Purify and recover pVAX1-HA2-fimA and empty vector pVAX1 with Promega plasmid extraction kit.

[0130] 2. Eighteen 6-8 week male SD rats were randomly divided into 3 groups, experimental group: group A, control group: group B, C, 6 rats in each group, as follows:

[0131] Group A: sample group pVAX1-HA2-fimA nasal instillation group;

[0132] Group B: no-load group pVAX1 nasal instillation group;

[0133] Group C: negative control group-normal saline nasal cavity instillation group;

[0134] 3. Immunization scheme: Immunization once a week, continuous immunization for 3 weeks, a total of 3 times of immunization, and the immunization dose of each rat was 200 μg / time. 4. Sample collection: Collect saliva samples 1 day before immunization and 1, 2, 3, and 4 weeks after immunization.

[0135] 2% pilocarpine nitrate (0.1g / mL) was inje...

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Abstract

The invention discloses novel DNA vaccine, a preparation method of the DNA vaccine as well as application of the DNA vaccine to preparation of medicines for preventing and treating periodontitis. TheDNA vaccine is pVAX1-HA2-fimA recombinant plasmid and consists of a core functional area HA2 of a porphyromonas gingivalis W83 type gingipain-hemagglutinin gene encoding area, a pilin gene fimA gene and a eukaryotic expression vector pVAX1 plasmid. The experiment proves that when the DNA vaccine constructed by the method is used for immunizing an SD rat, the DNA vaccine can successfully induce togenerate specific SIgA in saliva so as to induce an animal body to generate immune response, and has high immunogenicity so as to inhibit occurrence of SD rat periodontitis.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of oral diseases, and in particular relates to a DNA vaccine, a preparation method and its application in preventing and treating periodontitis. Background technique [0002] Periodontal disease is one of the main oral diseases that cause tooth loss, and it is also a major disease that endangers human oral and systemic health; chronic periodontitis is the main cause of loss of adult tooth function, with a high incidence. The damage caused by periodontal disease to periodontal tissue is continuous and irreversible. Once it occurs, it is difficult to achieve complete recovery with current treatment methods. In addition, periodontal disease is also closely related to certain systemic diseases, and is a risk factor for inducing certain systemic diseases such as diabetes and cardiovascular system diseases. Many clinical studies have shown that periodontal disease interacts with these s...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P1/02C12N15/85
CPCA61K39/00A61K2039/53C12N15/85C12N2800/107
Inventor 白国辉田源管晓燕喻正文刘建国王倩陈彬吴明松王方远
Owner ZUNYI MEDICAL UNIVERSITY
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