Inactivated yeast injection

A kind of injection, yeast technology, applied in the field of biomedicine

Active Publication Date: 2018-09-14
WEIFANG HUAYING BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research, we speculate that the injection made of inactivated yeast should also have an immune-enhancing effect on animal injections. After reviewing literature and patent databases, we found that there

Method used

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  • Inactivated yeast injection
  • Inactivated yeast injection
  • Inactivated yeast injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Inoculate Saccharomyces cerevisiae (purchased from China Industrial Microorganism Culture Collection Management Center; Latin name: Saccharomycescerevisiae, storage number: CICC1012) into the medium (weigh 20g peptone, 20g glucose, 10g yeast powder, add water to 1000mL, sterilize at 121°C 20 min), cultured in a constant temperature oscillator at 37°C at a speed of 160r / min for 24 hours, then centrifuged at 3000 rpm for 5 minutes, removed the supernatant culture solution to retain the precipitate, added sterile saline to wash the precipitate, centrifuged for 5 minutes, and repeated washing for 3 After several times, add sterile saline, mix with the precipitate, and make a suspension. Take a certain amount of Saccharomyces cerevisiae physiological saline suspension, and count the bacteria on the THOMA bacterial counting plate, so that each 1ml suspension contains 10 9 A Saccharomyces cerevisiae cell. The prepared physiological saline suspension of Saccharomyces cerevisia...

Embodiment 2

[0023] Torulaspora delbrueckii (Torulaspora delbrueckii, purchased from China Industrial Microorganism Culture Collection Management Center; Latin name: Torulaspora delbrueckii, preservation number: CICC1004) was inoculated into the medium (weighed 20g peptone, 20g glucose, 10g of yeast powder, add water to 1000mL, sterilize at 121°C for 20min to prepare), incubate in a constant temperature shaker at 37°C at a speed of 160r / min for 24 hours, then centrifuge at 3000rpm for 5 minutes, remove the supernatant culture solution and retain the precipitate, add sterile Wash the precipitate with normal saline, centrifuge for 5 minutes, repeat the washing 3 times, add sterile normal saline, mix with the precipitate, and make a suspension. Take a certain amount of torula spores physiological saline suspension, and count the bacteria on a THOMA bacterial counting plate, so that each ml suspension contains 10 9A Torula spp. thallus. The prepared Torula delbuspora saline suspension was ina...

Embodiment 3

[0025] The effects of the inactivated Saccharomyces cerevisiae injection prepared in Example 1 and the inactivated Torula delbui injection prepared in Example 2 on the phagocytic function of normal mouse monocyte-macrophages were detected by the carbon particle clearance test method. The clean grade Kunming mice weighing 18-22 g were divided into normal control group, inactivated Saccharomyces cerevisiae injection group and inactivated Torula cerevisiae injection group. There were 10 mice in each group, half male and half male. The mice in the normal control group were injected with sterile normal saline in the tail vein, the mice in the inactivated S. cerevisiae injection group were injected with the inactivated S. Torula spp. suspension. The administration volume of each of the above groups was 0.1 mL / 10 g, and the tail vein was injected continuously for 5 days, once a day. 2 hours after the last administration, the mice were injected with 0.05 mL / 10 g of Indian ink into t...

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PUM

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Abstract

The invention relates to an injection for enhancing immunologic function, resisting bacteria and resisting viruses, and belongs to the field of biological medicine. The medicine has the major ingredients of inactivated yeast and is prepared by inactivating living yeasts. Through the injection administration on the inactivated yeast, the body immunologic function can be enhanced; the antibacterialand antivirus effects can be achieved. The inactivated yeast injection can be used for prevention, treatment or auxiliary treatment of various diseases such as various bacterial diseases, viral diseases and low body immune function due to various reasons of people and animals; wide application prospects are realized.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to an injection for enhancing immune function, antibacterial and antiviral. Background technique [0002] Current studies have found that yeast has good probiotic properties such as regulating intestinal balance, promoting feed conversion, and improving the body's immune function, and is mostly used as feed additives for livestock and poultry breeding. Some studies have used yeast as a feed additive to enhance the immune function of pigs (patent publication number: CN106387398A). In addition, there are many studies on the use of yeast extract or its active ingredients (such as zymosan, zymosan oligosaccharide, and yeast β-glucan) to improve the immune function of the body (reference [1]: Molist F, Eerden E V, Parmentier H K, Vuorenmaa J. Effect of hydrolyzed yeast extract on immune function and growth performance of weaned piglets. Feed Expo, 2014, 12:32. Reference [2]: Ding Peng, Hu Gui...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K36/064A61P31/04A61P31/12A61P37/04
CPCA61K9/0019A61K36/064A61K39/0002A61K2039/521A61P31/04A61P31/12A61P37/04
Inventor 张勇石有斐
Owner WEIFANG HUAYING BIOTECH CO LTD
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