Bacillus simplex S62 and application thereof

A simple bacillus, S62 technology, applied in the field of microorganisms, can solve the problems of insufficient biological control technology of potato dry rot, and achieve the effects of low cost, environmental safety and wide source of raw materials

Inactive Publication Date: 2018-09-14
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a simple bacillus (Bacillus simplex) S62 obtained by separating and purifying from Chaerhan Salt Lake lake water, and the technical problem that is intended to solve is aimed at the deficiencies in current potato dry rot biological control technology

Method used

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  • Bacillus simplex S62 and application thereof
  • Bacillus simplex S62 and application thereof
  • Bacillus simplex S62 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The isolation and identification of embodiment 1 bacillus simplex S62

[0029] 1.1 Isolation and purification of Bacillus simplex S62

[0030] 1) Water sample pretreatment: Under sterile conditions, the water sample was mixed evenly, filtered with a filter equipped with a filter membrane with a pore size of 0.22 μm, and bacterial cells were enriched on the filter membrane. When the amount of water sample used for enrichment reaches 30ml, remove the filter membrane and put it into a glass test tube filled with 3ml of sterile seawater for 10 -1 watery.

[0031] 2) Soil sample pre-treatment: Take an appropriate amount of soil sample, air-dry (about 20 days), and treat at 120°C for 1 hour. Weigh 10g of the treated soil sample, add 90ml of sterile seawater, put it into a sterilized Erlenmeyer flask equipped with glass balls, shake it fully for 30min, and draw the supernatant after standing still, the supernatant is 10 -1 Soil sample. the above 10 -1 Samples were seriall...

Embodiment 2

[0059] Antagonism of Example 2 Bacillus simplex S62 to pathogenic fungi

[0060] 1.1 Bacteria culture

[0061] Inoculate 4 strains of pathogenic fungi (Qing 9A-4-13, Qing 9A-5-2, Qing 9A-5-10, 65B-2-6) into PDA medium, and culture them in a constant temperature incubator at 28°C for one week; Bacillus simplex S62 was inoculated on ATCC213 modified medium and cultured in a 37°C incubator for 1 day.

[0062] 1.2 Consistent training

[0063] Using the "cross-hatching method", the pathogenic fungus was punched into a bacterium cake with a diameter of 5 mm and placed at a distance of 2.5 cm from the center of Bacillus simplex S62 (colony diameter of 5 mm), and confronted culture was carried out in a petri dish with a diameter of 9 cm. The results are shown in Table 3.

[0064] Table 3 The confrontation culture between s62 and 4 strains of potato dry rot pathogenic fungi

[0065]

[0066] From Table 3 and figure 2 It can be obtained that the antagonistic effects of s62 on t...

Embodiment 3

[0067] Example 3 Re-screening of potatoes in vivo

[0068] 1.1 Preparation of fermentation broth

[0069] Inoculate the bacteria cultured in Example 2 into an Erlenmeyer flask filled with sterile water, place it in a constant temperature shaker at 37°C and 200r / min, and cultivate it for 5 days with shaking for 5 days before use.

[0070] 1.2 Preparation of bacterial suspension

[0071] Inoculate the bacteria cultured in Example 2 into an Erlenmeyer flask filled with sterile water, place it in a constant temperature shaker at 37°C and 200 r / min for 2 hours, shake well and set aside.

[0072] 1.3 Test method

[0073] Prick two diameters of 5mm and 3mm deep wounds on the sterilized potato fruit with a puncher with a diameter of 5mm (the potato fruit was soaked in 2% NaClO solution for 10min, rinsed with tap water, and dried naturally). Inoculate 40 μl of pathogenic bacteria suspension first, and then inoculate the same volume of Bacillus simplex S62 suspension. The control wa...

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Abstract

The invention discloses a bacillus simplex S62 and an application thereof. The strain is collected in a Guangdong Microbial Culture Collection Center of a Guangdong Institute of Microbiology on 5th Floor, No. 59 Building, No. 100 Xianliezhong Road, Guangzhou on September 12, 2016, and the collection number of the strain is GDMCC NO:60079. The strain and bacterial suspension or secondary metabolites of the strain have remarkable antagonistic effects on diseases caused by fusaria, material sources are wide, cost is low, and the bacillus simplex is safe for environments.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a strain of Bacillus simplex S62 derived from Chaerhan Salt Lake, in particular to Bacillus simplex S62 and an application thereof. Background technique [0002] Potatoes belong to the annual herb of Solanaceae Solanaceae, rich in nutrition, high in protein, vitamins and inorganic salts. With the launch of the potato staple food strategy in my country in 2015, the potato planting area continues to expand, and the occurrence of diseases is becoming more and more serious. Among them, Fusarium dry rot is one of the important diseases of potato during storage. It often leads to the decrease of potato tuber quality and the rate of commercial potato, seriously affecting its edible value and economic value, and has become the main factor restricting the development of potato industry. Among the many pathogenic fungi, Fusarium sambucinum and Fusarium solani were the main dominant pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12P1/04C12N1/205C12R2001/07
Inventor 沈硕李玮郭青云王舰他永全
Owner QINGHAI UNIVERSITY
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