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Cu<2+>-perylene tetracarboxylic acid assembly as well as preparation method and application

A technology of potassium perylene tetracarboxylate and unit potassium perylene tetracarboxylate is applied in the field of amino acid detection, which can solve the problems of high requirements, inability to provide real-time detection results quickly, and the like, and achieve the effect of high sensitivity

Active Publication Date: 2018-09-21
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, the detection of histidine mainly relies on high-performance liquid chromatography, capillary electrophoresis, ultraviolet-visible spectroscopy, and luminescence spectroscopy. Among them, high-performance liquid chromatography and capillary electrophoresis have high requirements for equipment and cannot quickly provide real-time detection. result

Method used

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  • Cu&lt;2+&gt;-perylene tetracarboxylic acid assembly as well as preparation method and application
  • Cu&lt;2+&gt;-perylene tetracarboxylic acid assembly as well as preparation method and application
  • Cu&lt;2+&gt;-perylene tetracarboxylic acid assembly as well as preparation method and application

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preparation example Construction

[0054] a Cu 2+ -The preparation method of potassium perylene tetracarboxylate assembly, the specific preparation method is as follows:

[0055] Cu was measured by the equimolar continuous change method (Job method) 2+ The binding ratio with potassium perylenetetracarboxylate is 2:1, so 2 mL of pure aqueous solution was added as a solvent in a 5 mL colorimetric tube, and 5 μL of PTAK aqueous solution (5×10 -3 mol L -1 ), then add 2 equivalents of Cu 2+ Coordinate with it to get Cu 2+ - Potassium perylene tetracarboxylate assembly.

[0056] A Cu-based 2+ -Application of potassium perylene tetracarboxylate assembly to selectively recognize histidine in a solvent system.

[0057] In order to further realize the technical effect of the present invention, in the solvent system, the volume percentage of water is 95-100%.

Embodiment 1

[0060] Synthesis of Monomer Compound (Potassium Perylene Tetracarboxylate)

[0061] 3,4,9,10-Perylenetetracarboxylic dianhydride (2mmol) and potassium hydroxide (8.8mmol) were mixed in pure water (30mL), heated to reflux until the solution became clear. After the reaction, the solvent water was distilled off under reduced pressure to obtain a yellow solid. Add absolute ethanol to the reaction crude product, and rinse with absolute ethanol several times after suction filtration to obtain the pure target product—potassium perylene tetracarboxylate.

[0062] For details, refer to the attached Figure 4-6 , are the hydrogen nuclear magnetic resonance spectrum, carbon nuclear magnetic resonance spectrum and infrared spectrum (potassium bromide tablet) of potassium perylene tetracarboxylate in heavy water, respectively.

[0063] which is combined with Figure 4 for 1 H NMR (500MHz,D 2 O) spectrum, the specific spectrum peaks are: δ8.27 (d, J=7.9Hz, 4H), 7.65 (d, J=7.9Hz, 4H). ...

Embodiment 2

[0067] Cu 2+ -Synthesis of PTAK assemblies

[0068] To 2mL potassium perylenetetracarboxylate (1.25×10 -5 mol L -1 ) solution by adding 0.5μL CuSO 4 ·5H 2 O aqueous solution (0.1mol·L -1 ), that is, mixed according to the molar ratio of 1:2 to obtain Cu 2+ - Aqueous solution of PTAK assembly.

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Abstract

The invention discloses a Cu<2+>-perylene tetracarboxylic acid assembly as well as a preparation method and application. The Cu<2+>-perylene tetracarboxylic acid assembly disclosed by the invention has the competitive bonding of perylene tetracarboxylic potassium and histidine with Cu<2+> as a basic action mechanism, and in addition, perylene tetracarboxylic potassium light emission is adopted asa fluorescent signal report group. When being put into a system, histidine can be in coordinate bonding of Cu<2+> in an assembly structure, then the assembly structure can be decomposed, perylene exists in a mode of monomer dispersion in a bulk phase solution instead of ordered accumulation, and solution color and fluorescence change can be resulted. Recognition results of the assembly upon multiple amino acids are studied by using a colorimetric method, an ultrasonic-visible absorption spectrum method and a fluorescent spectrum method, and results show that the Cu<2+>-perylene tetracarboxylicacid assembly is capable of effectively and selectively recognizing histidine in a pure water solvent system, and has very high sensitivity to histidine.

Description

technical field [0001] The invention belongs to the technical field of amino acid detection, relates to a method strategy for detecting histidine, in particular to a Cu-based 2+ - Metal-coordinated supramolecular assemblies of potassium perylenetetracarboxylate. The invention also relates to the synthesis method of the assembly unit and its application in detecting histidine. Background technique [0002] The structure of histidine contains an imidazole group, which is an essential amino acid for the growth and development of young children. In addition, in life activities, histidine is a neurotransmitter and has a regulatory role in metal transport. The content of histidine is closely related to the health index of the human body. Too little intake of histidine may cause diseases such as chronic kidney disease, acute liver failure and rheumatoid arthritis. Too much histidine in the life system may be related to psychological disorders. Therefore, the efficient and conveni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/41C07C63/49C08G83/00C09K11/06G01N21/31G01N21/64G01N21/78
CPCG01N21/314G01N21/6486G01N21/78C09K11/06C07C51/418C07C63/49C08G83/008G01N2021/3155C07C2603/52C09K2211/1416C09K2211/1425C09K2211/1011
Inventor 张鹏张倩徐玉娟丁彩凤
Owner QINGDAO UNIV OF SCI & TECH