Pregnane glycoside compounds and their application
A technology of pregnane glycosides and compounds, applied in the field of pregnane glycoside compounds
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Example 1: Preparation of Epigycoside B
[0020] Burmese Simao vine ( Epigynum cochinchinensis ) branches and leaves, and 5 kg of the obtained leaves were dried naturally in a dry and ventilated place, and then crushed into pieces with a powder machine; the Burmese Simao vine leaf fragments were immersed in 10 L of methanol solution with a volume concentration of 75% for extraction, each time for 12 hours, continuous Leach three times; collect and combine the extracts, use a rotary evaporator to concentrate and remove the liquid under reduced pressure, and obtain 500 g of extract; add 2 L of water to dissolve the extract, put it in a separatory funnel, and then add an equal volume of ethyl acetate for repeated extraction ; Repeatedly extracted until the ethyl acetate layer was almost colorless, collected and combined the ethyl acetate layer and evaporated to dryness to obtain 120 g of the crude extract extract; using a medium pressure chromatographic separation system,...
Embodiment 2
[0030] Embodiment 2: Immunosuppression detection test
[0031] (1) Preparation of spleen lymphocyte suspension
[0032]18-22g healthy BABL / c mice were sacrificed by removing the eyeballs for bloodletting and then sacrificing them by taking off the neck, soaking them in 75% alcohol for 3-5 minutes, taking them out and placing them in a sterile tray with the left side of the abdomen facing upwards. In the cabinet, use sterilized tweezers to pick up the fur in the middle of the abdomen, make an incision from the lower end, use another set of instruments to cut open the layers of the abdominal wall, use the third set of instruments to remove the spleen, remove fat and connective tissue, and put it in PBS (Phosphate buffered saline) to wash away the floating blood. Then move the spleen tissue to a plate filled with RPMI1640 incomplete culture medium, cut it into small pieces with scissors, use a sterile syringe core to gently grind the spleen in a 200-mesh stainless steel screen, ...
Embodiment 3
[0060] Embodiment 3: in vitro anti-inflammatory activity test
[0061] (1) Preparation of test-related reagents
[0062] Preparation of sample dilution working solution: The concentrated sample dilution solution was taken out from the -4°C refrigerator, thawed slowly at room temperature, and diluted with double distilled water at a ratio of 1:5 according to the amount required for the test.
[0063] Preparation of reagent dilution working solution: take the concentrated reagent solution out of the -4°C refrigerator, thaw slowly at room temperature, take part according to the required amount of the test, and dilute it with double distilled water at a ratio of 1:5.
[0064] Preparation of cell lysis working solution: The concentrated cell lysate was taken out from the -4°C refrigerator, thawed slowly at room temperature, and diluted with double distilled water at a ratio of 1:2 according to the amount required for the test.
[0065] Preparation of washing working solution: Take...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More