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Photoelectric chemical sensor for detecting sulfadimethoxine as well as detection method thereof

A sulfadixine, photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of low detection sensitivity, expensive operation of instruments, and the need for professional operators, and achieve high detection sensitivity, Simple detection method and high detection selectivity

Inactive Publication Date: 2018-09-21
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have the disadvantages of low detection sensitivity, low detection specificity, high detection cost, expensive instruments, complicated operation, and the need for professional operators. Therefore, it is necessary to establish a simple, fast, high sensitivity and high selectivity method. , for the clinical diagnosis and food safety analysis of sulfadisoxine, is crucial
There is no report on the detection of sulfadisoxine by photoelectrochemical analysis

Method used

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  • Photoelectric chemical sensor for detecting sulfadimethoxine as well as detection method thereof
  • Photoelectric chemical sensor for detecting sulfadimethoxine as well as detection method thereof
  • Photoelectric chemical sensor for detecting sulfadimethoxine as well as detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: The preparation of the photoelectrochemical sensor that is used to detect sulfadisoxine

[0046] 1. Electrode pretreatment:

[0047] First divide the ITO conductive glass into 5×1cm 2 , and ultrasonically cleaned with ethanol / NaOH mixture (1:1) for 30 minutes, and finally washed with acetone and secondary water for 30 minutes respectively, and dried at room temperature for use.

[0048] 2. WS 2 Preparation of nanosheets:

[0049] WS prepared by ultrasonic-assisted chemical exfoliation 2 Nanosheets. Weigh 1 gram of WS 2 Powder material, added to 200mL deionized water, ultrasonically dispersed for 30 minutes to obtain WS 2 water dispersion. Then add 2 g sodium cholate to WS 2 In the aqueous dispersion, stir for 30 minutes. Subsequently, the dispersion was sonicated for 24 hours. Centrifuge at 3000 rpm for 30 minutes, discard the precipitate, and collect the solution. The collected solution was centrifuged at 12000 rpm to collect the precipitate. ...

Embodiment 2

[0056] Embodiment 2: the detection of sulfadisoxine

[0057] 1. Sulfadesoxine and aptamer binding reaction, and isothermal amplification reaction catalyzed by DNase I:

[0058] Sulfadisoxine solutions of different concentrations were mixed with 100 unit / mL DNase I solution in equal volumes. Then, 10 μl of this mixed solution was added dropwise to the DNA / WS 2 / ITO electrode surface, stand in a humid environment (humidity 99%) for 2 hours at a temperature of 37°C. After washing with 10mmol / L Tris-HCl (pH 7.4) buffer solution, the obtained electrode is labeled as: DNA-C / WS 2 / ITO.

[0059] 2. Photoelectrochemical detection:

[0060] Using an electrochemical workstation as a signal acquisition instrument, a 500W xenon lamp as a visible light source (installed with a lens to filter out ultraviolet rays), a saturated calomel electrode as a reference electrode, a platinum column electrode as a counter electrode, and 10mM Tris-HCl (pH 7.4) buffer solution As the detection soluti...

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Abstract

The invention discloses a photoelectric chemical sensor for detecting sulfadimethoxine as well as a detection method thereof. The photoelectric chemical sensor comprises an electrode, a WS2 nano-sheetand a DNA aptamer; the WS2 nano-sheet and the DNA aptamer are modified on the surface of the electrode and are tightly contacted. The specificity and the sensitivity of the target compound sulfadimethoxine are detected by utilizing the specificity identification effect of the DNA nucleic acid aptamer and the sulfadimethoxine and performing DNase I catalyzed signal isothermal amplication, and a new method is provided for the fields of environment monitoring, food safety and the like.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical analysis, in particular to a photoelectrochemical sensor for detecting sulfidesoxine and a detection method thereof. Background technique [0002] Sulfadesoxine belongs to the sulfonamide antibiotics, has strong antibacterial and protozoan infection effects, can be used for upper respiratory tract and urinary tract infections, and is currently widely used in the treatment and prevention of livestock and poultry diseases; in addition, it has a positive effect on the growth of livestock and poultry. It is widely used as a feed additive. Due to the existence of abuse and non-compliance with the withdrawal period, sulfadisoxine has a certain residue in animal food, which is potentially harmful to food hygiene and human health. [0003] The current detection methods for sulfidesoxine are mainly ultra-high performance liquid chromatography, high performance liquid chromatography, liquid ch...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/3278
Inventor 殷焕顺周云雷艾仕云隋成吉王月
Owner SHANDONG AGRICULTURAL UNIVERSITY