Small molecular monocarboxylic acid derivatization-liquid chromatography detection method

Abstract

The invention discloses a small molecular monocarboxylic acid derivatization-liquid chromatography detection method. Chromatographic conditions are as follows: the quantitative detection wavelength is223nm, a chromatographic column is Agilent SB-C18, and the temperature of a column oven is 35 DEG C; acetonitrile and water are used as a mobile phase, and the fixed flow speed is 0.8mL / min; the gradient elution procedure is as follows: the volume ratio of the acetonitrile is 10% at 0 minute, is increased to 70% at 30 minutes and is reduced to 10% at 32 minutes, and the procedure ends at 34 minutes. Several monocarboxylic acid derivatization-liquid chromatography detection methods provided by the invention have qualitative and quantitative accuracy, have the sensitivity which is at least 2 orders of magnitude higher than that in a direct method, can be applied to detection of butyric acid, and have very obvious advantages.

Description

technical field [0001] The invention belongs to an organic acid detection technology in related fields such as agriculture, ecology and environment, and in particular relates to high-sensitivity instrumental analysis and detection of several low-content monocarboxylic acids in ecological environment water samples and plant rhizosphere samples. Background technique [0002] Organic acids widely exist in nature. Small molecule organic acids in the rhizosphere mainly come from the decomposition of animal and plant residues, microbial metabolism, secretion and decomposition of plant roots, and transformation of organic matter applied to the soil. It is a kind of functional organic matter ubiquitous in soil and the most active form of carbon in soil. It can affect the chemical and biological processes of soil through complex mechanisms such as chelation and ligand exchange. The effectiveness of mineral nutrients in the rhizosphere, the mitigation of acidic soil aluminum toxicity ...

AI Technical Summary

Problems solved by technology

But their main disadvantage is that the complex components in the actual sample will affect the accurate qualitative and quantitative detection of the target object, and it is difficult to detect the low content sample

If a dedicated organic acid determination column is used, the chromatographic column is expensive, and the pretreatment of the sample is complicated, the sample needs to be removed from cations, etc., and there is also the problem of poor detection sensitivity of the instrument; when using the IC method to analyze organic acids, Some organic acids are affected by negative water peaks, making qualitative and quantitative analysis difficult

The CE rule mainly shows that the amount of sample is small, the analysis time is short, and the interference is smaller than that of the HPLC method and the IC method, but the stability of the instrument is worse than that of the HPLC method and the IC method, and there is also detection sensitivity for low-content organic acid samples. The problem is low, and the operating cost is relatively high

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