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Application of ahl molecule as chemical pesticide bactericidal synergist in the control of diseases caused by Dickia maize

A technology of Dickia maize and chemical agents, which is applied in the field of biological control technology and plant protection, and can solve problems such as long synthetic routes and environmental pollution

Active Publication Date: 2020-10-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional synergists mainly improve the bactericidal effect of pesticides by improving the physical and chemical properties of pesticides such as wetting and permeability, but their synthesis routes are long and pollute the environment
At present, there is no relevant report on the development of pesticide synergists with small molecule AHL

Method used

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  • Application of ahl molecule as chemical pesticide bactericidal synergist in the control of diseases caused by Dickia maize
  • Application of ahl molecule as chemical pesticide bactericidal synergist in the control of diseases caused by Dickia maize
  • Application of ahl molecule as chemical pesticide bactericidal synergist in the control of diseases caused by Dickia maize

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of AHL Small Molecules

[0027] The overnight cultured Pseudomonas aeruginosa seed solution was inserted into YEB medium, 28 o C. After culturing at 200 rpm for 24 hours, the culture supernatant was collected by centrifugation. The obtained supernatant is to be used for the extraction of small molecules. Among them, the formula of YEB medium is: 0.5 g / LMgSO 4 •7H 2 O, 5 g / L Sucrose, 5 g / L Yeast Extract, 10 g / L Trypton, 5 g / L NaCl, pH 7.0.

[0028] The present invention adopts the conventional compound separation and extraction method to separate and extract the active substances in the fermentation broth. After collecting the supernatant of the culture medium by centrifugation, add the same volume of ethyl acetate as the supernatant, put it at 4°C for 2h, then shake at 20°C for 1h, extract with a separatory funnel, and collect the supernatant. The collected upper layer was then rotary evaporated using a rotary evaporator, the organic solvent w...

Embodiment 2

[0029] Example 2 Bactericidal and synergistic effect of small molecule AHL and copper thiophanate on D. zeae

[0030] Colony counting method (CFU method) was used to determine the bactericidal and synergistic effect of small molecule AHL extracts at different concentrations on copper thiophanate. The small molecule AHL was prepared and diluted to a certain concentration with methanol as a solvent, and cultured to OD overnight. 600 =1.0 EC1 Bacterial solution, add thiophanate copper diluted 500 times at 100 mg / ml, set a treatment without small molecule AHL as a positive control, and set up a series of small molecule AHL with concentration gradient (40 μM, 80 μM, 160 μM, 320 μM, 500 μM) and Treatment of thiophanate-copper mixes. All processed at 28 o C. After culturing at 200rpm for 24 hours, dilute 10 4 times, 200 μL of bacterial liquid was taken from the plate (the medium was LB), and placed in a constant temperature incubator at 28 °C for cultivation. Observe and count t...

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Abstract

The invention discloses an application of a small-molecule compound produced by bacteria as a synergist of a chemical pesticide bactericide. The synergist is 3-oxydodecanoyl homoserine lactone (AHL) generated by pseudomonas aeruginosa (PA), and can be extracted from a fermentation broth of the pseudomonas aeruginosa or can be synthesized by artificial chemistry. A micro amount of the small-molecule compound (AHL) is mixed with the chemical pesticide bactericide for use, and has an obvious synergistic effect on prevention and control of the diseases caused by Erwinia chrysanthemi pv.Zeae. The compound disclosed by the invention can significantly improve a bactericidal effect of the chemical pesticide bactericide, greatly reduce a use amount of chemical pesticides and residues of the pesticides, can reduce costs, protect the environment, effectively alleviate the increasingly-serious problem of drug resistance of pathogenic bacteria, and promote achievement of a ''pesticide reduction'' indicator and green development of agriculture.

Description

technical field [0001] The present invention relates to Pseudomonas aeruginosa Pseudomonas aeruginosa, PA The resulting AHL small molecule acts as a novel synergist for chemical pesticide fungicides against D. zeae Erwiniachrysanthemi pv . Zeae The bactericidal and synergistic effect of the invention belongs to the field of biological control technology plant protection. Background technique [0002] D. zeae Erwiniachrysanthemi pv. Zeae It is a worldwide important plant pathogenic bacteria with a wide range of hosts, which can infect Araceae, Crassulaceae, Cactaceae, Euphorbiaceae, Liliaceae, Gesneriaceae, Caryophyllaceae, Nebaceae, Yanaceae, Chrysanthemum There are 21 species of plants in 14 families, such as family, Luanaceae, Chrysanthinaceae, Cruciferae, Umbelliferae, etc., which mainly cause soft rot, which seriously affects the development of my country's agricultural industry. in D. zeae The bacterial base rot of rice and the bacterial soft rot of bananas ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/824A01N43/08A01P1/00
CPCA01N43/08A01N43/82
Inventor 常长青周佳暖廖金星仇善旭章婉贤王雨婷
Owner SOUTH CHINA AGRI UNIV