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Application of foxd1 in preparation of products for treating and/or preventing animal osteoarthritis

A technology for osteoarthritis and products, applied in the field of biomedicine, can solve the problems of long-term expression, cytotoxicity, and large capacity

Active Publication Date: 2021-08-17
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adeno-associated virus has a long expression time and high safety, but it also has the disadvantages of small capacity for carrying foreign genes and difficult preparation
Herpes simplex virus has a large capacity to carry foreign genes, but it is cytotoxic and cannot be expressed for a long time

Method used

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  • Application of foxd1 in preparation of products for treating and/or preventing animal osteoarthritis
  • Application of foxd1 in preparation of products for treating and/or preventing animal osteoarthritis
  • Application of foxd1 in preparation of products for treating and/or preventing animal osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1, FOXD1 pair of delayed effects of meta-mesencholic stem cell aging

[0128] 1.1 Compact aging with mesenchymal stem cells, FoxD1 expression is obviously lowered

[0129] Using vitro-directional differentiation techniques, human mesenchymal stem cells obtained from human embryonic stem cells can be continuously passed from the first generation under vitro culture conditions. The 12th generation of mesenchymal stem cells have obvious growth block, and the cell volume is large, and the morphological fraction of cellular aging phenotype. As the second generation and the 12th generation of mesenchymal stem cells as the test cell, the second generation of cells represent young cells, and the 12th generation cell represents aging cells. The total protein of the two cells was extracted, and the cells were tested using the Ripa cell lysate (Biyumi, P0013B), and then the protein concentration in the cells were determined using a BCA protein quantitative kit (Biyun, P0010), ...

Embodiment 2

[0166] Example 2. The therapeutic effect of FOXD1 gene to cut off osteoarthritis in the former cross ligament

[0167] Since the encoding FOXD1 lentivirus obtained in Example 1 has a good delayed mesenchymal stem cell aging in vitro, the encoding FOXD1 lentivirus obtained in Example 1 is used in the pre-interstitial ligament to interrupted osteoarthritis mice. Genetic therapy.

[0168] 2.1 Establishing a front cross-ligament cut - induced osteoarthritis mouse model

[0169] The mice used were SPF grade, male, 10 weeks, weight 20 ± 2g, Beijing Huayang Biotechnology Co., Ltd. Experimental instruments and materials include: isofluflufluorane gas anesthesia, hair removal, surgical scissors, surgical knife, Venus shearing, suture, penicillin, body mirror, 20 μL of microsampler.

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Abstract

The invention discloses the application of FOXD1 in preparing products for treating and / or preventing animal osteoarthritis. The present invention finds that FOXD1 has the following functions: treating and / or preventing animal osteoarthritis; treating and / or preventing animal anterior cruciate ligament cut-induced osteoarthritis; delaying the aging of mesenchymal stem cells; delaying the replication of mesenchymal stem cells Sexual aging. Experiments have shown that the knockdown of FOXD1 in mesenchymal stem cells in vitro accelerated the aging of mesenchymal stem cells, while the overexpression of FOXD1 delayed the aging of mesenchymal stem cells; in vivo experiments confirmed that FOXD1 gene can reduce the degeneration of articular cartilage and joint Inflammatory response, which has a good therapeutic effect in osteoarthritic diseases. The invention provides a new idea for the development of gene therapy for osteoarthritis, and expands the optional range of clinical gene therapy.

Description

Technical field [0001] The present invention relates to the application of FOXD1 in the field of biomedicine, in the preparation of treatment and / or prevention of animal osteoarthritis products. Background technique [0002] OsteoartHritis, OA is the most common chronic degenerative joint lesions, and clinical manifestations are chronic joint pain, stiffness, hypertrophy and activities. The incidence of osteoarthritis is related to age, obesity, inflammation, trauma, genetic and other factors. The disease occurs in the middle-aged and older, and the prevalence increases with age, and in addition, the incidence of women is higher than men. Due to extremely high prevalence and disability ratio, osteoarthritis has become one of the major diseases that endanger human health and affect social development. [0003] At present, the treatment of osteoarthritis still uses drug therapy. Compared to systemic administration, the joint cavity can cause the drug direct area, reduce the drug ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K38/17A61P19/02A61P29/00
CPCA61K38/1709A61K48/005A61P19/02A61P29/00
Inventor 曲静刘光慧付丽娜任若通
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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