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Three-layer artificial blood vessel electrospinning membrane loaded with micro nucleic acid as well as preparation method and application thereof

An artificial blood vessel and micro nucleic acid technology, which is used in medical science, prosthesis, etc. to inhibit excessive proliferation, reduce the risk of restenosis, and facilitate cell adhesion.

Active Publication Date: 2018-10-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention has good application prospects in the field of small-diameter artificial blood vessel materials, and this work has not been reported so far

Method used

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  • Three-layer artificial blood vessel electrospinning membrane loaded with micro nucleic acid as well as preparation method and application thereof
  • Three-layer artificial blood vessel electrospinning membrane loaded with micro nucleic acid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The preparation method of the above-mentioned three-layer artificial blood vessel loaded with microRNAs is characterized in that it includes the following process:

[0033] (1) Dissolve polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) and PELCL-REDV in chloroform and N,N- In the mixed solution of dimethylformamide, the volume ratio of chloroform:DMF is (4~8):1, and the concentration is 100~200mg / mL solution is used as the oil phase, and the carrier TMC-g-PEG-REDV is used as N / P The ratio is (12-18): 1, load miRNA-126 as the water phase, and stir to prepare a stable emulsion.

[0034] (2) Dissolve poly(glycolide-co-lactide) in a mixed solution of chloroform and N,N-dimethylformamide, the volume ratio of chloroform:DMF is (4~8):1, and configure the concentration A 150-250 mg / mL solution was used as the oil phase, and the carrier TMC-g-PEG-VAPG was loaded with miRNA-145 at an N / P ratio of (12-18):1 as the water phase, and stirred to prepare a stable emulsion.

[0...

Embodiment 1

[0044] Polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) and PELCL-REDV Dissolve in the mixed solution of chloroform and N,N-dimethylformamide according to the mass ratio of 1:1, the volume ratio of the solvent is 4:1, and the solution with a concentration of 150mg / mL is used as the oil phase, and the carrier TMC-g- PEG-REDV was loaded with miRNA-126 at an N / P ratio of 12:1 as the aqueous phase and stirred to prepare a stable emulsion. Poly(glycolide-co-lactide) Dissolve in the mixed solution of chloroform and N,N-dimethylformamide, the solvent volume ratio is 4:1, and the concentration is 200mg / mL solution as the oil phase, with the carrier TMC-g-PEG-VAPG according to N / P The ratio of 12:1 was loaded with miRNA-145 as the water phase, and stirred to prepare a stable emulsion. polyε-caprolactone Dissolve in the mixed solution of chloroform and N,N-dimethylformamide, the solvent volume ratio is 4:1, and the concentration is 200mg / mL.

[0045] The resulting soluti...

Embodiment 2

[0048] Polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) and PELCL-REDV According to the mass ratio of 2:1, dissolve in the mixed solution of chloroform and N,N-dimethylformamide, the solvent volume ratio is 6:1, and the solution with a concentration of 100mg / mL is used as the oil phase, and the carrier TMC-g- PEG-REDV was loaded with miRNA-126 at an N / P ratio of 15:1 as the aqueous phase and stirred to prepare a stable emulsion. Poly(glycolide-co-lactide) Dissolve in the mixed solution of chloroform and N,N-dimethylformamide, the solvent volume ratio is 6:1, and the concentration is 150mg / mL solution as the oil phase, with the carrier TMC-g-PEG-VAPG according to N / P The ratio of 15:1 was loaded with miRNA-145 as the water phase, and stirred to prepare a stable emulsion. polyε-caprolactone Dissolve in the mixed solution of chloroform and N,N-dimethylformamide, the solvent volume ratio is 6:1, and the concentration is 200mg / mL.

[0049] The resulting solution was ...

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Abstract

The invention relates to a three-layer artificial blood vessel electrospinning membrane loaded with micro nucleic acid as well as a preparation method and application thereof. An artificial blood vessel is composed of three electrospinning membranes; an inner membrane is polyethylene glycol-b-poly(L-lactide-co-epsilon-caprolactone)electrospinning fiber membrane and is internally loaded with particles formed by trimethylchitosan-grafted-polyethylene glycol-REDV compound miRNA-126 and PELCL-REDV; a middle membrane is a poly(glycolide-co-lactide)electrospinning fiber membrane and is internally loaded with particles formed by trimethylchitosan-grafted-polyethylene glycol-VAPG compound miRNA-145; and an outer membrane is a poly(epsilon-caprolactone)electrospinning fiber membrane. The three-layer artificial blood vessel is capable of effectively controlling the release rate of two miRNAs; the risk of later-period restenosis during vascular remodeling is reduced; the three-layer artificial blood vessel electrospinning membrane can be applied to the fields of artificial blood vessel materials and blood vessel transplantation biomedical materials.

Description

technical field [0001] The invention relates to a three-layer artificial blood vessel electrospun fiber membrane loaded with tiny nucleic acids, a preparation method and application thereof, and belongs to the field of tissue engineering and biomedical materials. Background technique [0002] Artificial blood vessel substitutes are a common clinical treatment for vascular blockage, but small-diameter artificial blood vessels with an inner diameter of less than 6 mm are still a difficult problem to be solved due to restenosis (Pashneh-Tala S, MacNeil S, Claeyssens F. The tissue -engineered vascular graft-past, present, and future. Tissue Engineering Part B-Reviews, 2016, 22(1): 68-100). Electrospinning method can be used to prepare ultrafine fiber membranes, which can simulate extracellular matrix. The aliphatic polyester materials poly(L-lactide-co-caprolactone) (PLCL), poly(glycolide-co-lactide) (PLGA) and polyε-caprolactone (PCL) all have Good biocompatibility and mechan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/20A61L27/22A61L27/54A61L27/50
CPCA61L27/18A61L27/20A61L27/227A61L27/50A61L27/54A61L2300/258A61L2300/602C08L71/02C08L5/08C08L89/00C08L67/04
Inventor 袁晓燕文美玲崔策周芳赵蕴慧
Owner TIANJIN UNIV
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