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Efficient culture medium for chlorella

A culture medium and chlorella technology, applied in the field of culture medium, can solve the problems of being easily affected by natural conditions and low yield, and achieve the effects of reducing production costs, high yield, and fast growth rate

Inactive Publication Date: 2018-10-09
日照澳可生生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, autotrophic culture is mostly used. The autotrophic culture of Chlorella can use both natural light and artificial light. The medium is mainly composed of inorganic compounds. The traditional culture method has low yield and is easily affected by natural conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A high-efficiency medium for chlorella, each liter of high-efficiency medium for chlorella includes the following components:

[0033] (1) Macroelements: 0.10g sodium nitrate, 0.01g potassium dihydrogen phosphate, 0.02g magnesium sulfate heptahydrate, 0.02g urea, 0.01g calcium chloride, 0.01g sodium bicarbonate, 0.50g sodium chloride;

[0034] (2) Trace elements: 2.00mg ferric citrate, 0.05mg copper sulfate pentahydrate, 2.00mg boric acid, 1.50mg manganese chloride tetrahydrate, 0.10mg sodium molybdate dihydrate;

[0035] The remaining components of the high-efficiency culture medium for chlorella are seawater.

[0036] The preparation method of described chlorella high-efficiency culture medium, the steps are as follows:

[0037] 1) Weigh each raw material according to the proportion of the above formula, and set aside;

[0038] 2) Dissolve and mix the above required amount of sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, urea, calci...

Embodiment 2

[0046] A high-efficiency medium for chlorella, each liter of high-efficiency medium for chlorella includes the following components:

[0047] (1) Macroelements: 0.15g sodium nitrate, 0.02g potassium dihydrogen phosphate, 0.03g magnesium sulfate heptahydrate, 0.04g urea, 0.02g calcium chloride, 0.03g sodium bicarbonate, 0.8g sodium chloride;

[0048] (2) Trace elements: 3.00mg of ferric citrate, 0.08mg of copper sulfate pentahydrate, 2.50mg of boric acid, 1.80mg of manganese chloride tetrahydrate, 0.20mg of sodium molybdate dihydrate;

[0049] The remaining components of the high-efficiency culture medium for chlorella are seawater.

[0050] The preparation method of described chlorella high-efficiency culture medium, the steps are as follows:

[0051] 1) Weigh each raw material according to the proportion of the above formula, and set aside;

[0052] 2) Dissolve and mix the above required amount of sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrat...

Embodiment 3

[0060] A high-efficiency medium for chlorella, each liter of high-efficiency medium for chlorella includes the following components:

[0061](1) Macroelements: 0.16g sodium nitrate, 0.02g potassium dihydrogen phosphate, 0.05g magnesium sulfate heptahydrate, 0.04g urea, 0.02g calcium chloride, 0.02g sodium bicarbonate, 1.00g sodium chloride;

[0062] (2) Trace elements: 4.00mg ferric citrate, 0.08mg copper sulfate pentahydrate, 2.86mg boric acid, 1.81mg manganese chloride tetrahydrate, 0.21mg sodium molybdate dihydrate;

[0063] The remaining components of the high-efficiency culture medium for chlorella are sea water.

[0064] The preparation method of described chlorella high-efficiency culture medium, the steps are as follows:

[0065] 1) Weigh each raw material according to the proportion of the above formula, and set aside;

[0066] 2) Dissolve and mix the above required amount of sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, urea, calci...

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Abstract

The invention discloses an efficient culture medium for chlorella. The efficient culture medium for the chlorella comprises the following components per liter: (1) major elements: 0.10-0.20g of sodiumnitrate, 0.01-0.04g of potassium dihydrogen phosphate, 0.02-0.08g of magnesium sulfate heptahydrate, 0.02-0.06g of urea, 0.01-0.04g of calcium chloride, 0.01-0.05g of sodium bicarbonate and 0.50-1.5gof sodium chloride; (2) microelements: 2.00-5.00mg of ferric citrate, 0.05-0.10mg of copper sulfate pentahydrate, 2.00-3.00mg of boric acid, 1.50-2.00 mg of tetrahydrate manganese chloride and 0.10-0.40 mg of sodium molybdate dihydrate; and the balance of seawater. The efficient culture medium for the chlorella can be directly added and applied, so that the chlorella can quickly absorb various nutrients in a liquid medium in a short time, thereby realizing high-density culture, and achieving faster growth rate and higher yield; at the same time, the cost of buying agar powder is saved, thereby reducing the production cost.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to a high-efficiency medium for chlorella. Background technique [0002] Chlorella is a general unicellular green algae belonging to the phylum Chlorella. It is a spherical single-celled freshwater algae with a diameter of 3 to 8 microns. It is one of the earliest life on earth and appeared more than 2 billion years ago. The gene has not changed all the time, and it is a highly efficient photosynthetic plant that grows and reproduces with photosynthetic autotrophy and is widely distributed. [0003] The nutritional components of Chlorella Chlorella cells are rich in photosynthetic pigments, so they have strong photosynthetic production capacity, and their cells are rich in polysaccharides, proteins, pigments, vitamins, minerals, etc., corresponding to this is a A series of health functions and pharmacological effects. Nutrient composition of chlorella -- protein Chlorella has a hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 梁金培王小花刘军丁河峰
Owner 日照澳可生生物科技有限公司
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