Bacterial strain for producing bacterial cellulose and isolation and screening method thereof

A bacterial cellulose and screening method technology, applied in the field of microorganisms, can solve the problems of low fermentation yield and high production cost

Active Publication Date: 2018-10-09
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, so far, there are still some problems in the large-scale production of bacterial cellulose, mainly low fermentation yield and high production cost.

Method used

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  • Bacterial strain for producing bacterial cellulose and isolation and screening method thereof
  • Bacterial strain for producing bacterial cellulose and isolation and screening method thereof
  • Bacterial strain for producing bacterial cellulose and isolation and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: a screening of high-yield bacterial cellulose

[0053] Take about 1g of the rotten part of the fruit, put it into a 18mm×180mm test tube filled with 10mL primary enrichment medium after sterilization, and culture it statically at 30°C for 5 days. There will be a milky white colloidal film on the liquid surface in the tube and the film will not be broken. are positive. Take the film and wash it with 0.9% sterile saline for 3 times, cut it into pieces and place it in a 18mm×180mm test tube filled with 10mL enrichment medium for shaking for 1h, take the shaking solution and spread it on a HS fluorescence screening plate for 5d after gradient dilution Finally, under the irradiation of a 365nm ultraviolet lamp, the plate was irradiated by a 365nm ultraviolet lamp, and a large single colony with obvious fluorescence was picked and cultured in a small test tube containing 2mL of a secondary enrichment medium with a pH value of 7 for 5 days, and the pH of the cult...

Embodiment 2

[0054] Embodiment 2: a screening of high-yield bacterial cellulose (screening pH control example)

[0055] Take about 1g of the rotten part of the fruit, put it into a 18mm×180mm test tube filled with 10mL primary enrichment medium after sterilization, and culture it statically at 30°C for 5 days. There will be a milky white colloidal film on the liquid surface in the tube and the film will not be broken. are positive. Take the film and wash it with 0.9% sterile saline for 3 times, cut it into pieces and place it in a 18mm×180mm test tube filled with 10mL enrichment medium for shaking for 1h, take the shaking solution and spread it on a HS fluorescence screening plate for 5d after gradient dilution Finally, under the irradiation of a 365nm ultraviolet lamp, the plate was irradiated by a 365nm ultraviolet lamp, and a large single colony with obvious fluorescence was picked and cultured in a small test tube containing 2 mL of a secondary enrichment medium with a pH value of 5 fo...

Embodiment 4

[0065] Embodiment 4: the fermentation of the primary screening bacterial strain of embodiment 2

[0066] The bacterial cellulose-producing control strain (Gluconacetobacter xylinus CGMCC1.1812) preserved on the slope and the 42 cellulose-producing bacterial strains in Example 2 were taken out for activation, and other operations were the same as in Example 3. Compared with the yield of the obtained 42 strains and the control strain Gluconacetobacter xylinus CGMCC 1.1812, only 4 strains exceeded the control strain, and the ratio was 9.5%. ( image 3 )

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PUM

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Abstract

The invention discloses a novel bacterial strain for producing bacterial cellulose and an isolation and screening method of the bacterial strain. The bacterial strain is named as Komagataeibacter hansenii Man-170518-hww and is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC No.15468. The bacterial strain is separated from mangos, and is a gram negative and short-rod-shaped aerobic bacterium; stable high-yield capability is realized; a large quantity of bacterial cellulose can be produced during the baffle plate triangular flask fermentation.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterial strain producing bacterial cellulose and an isolation and screening method thereof. Background technique [0002] Bacterial cellulose is an ultra-pure ultrafine cellulose synthesized by bacteria. It is a chain polymer formed by D-glucose linked by β-1,4 glycosidic bonds. Compared with plant cellulose existing in nature, it is superior to plant cellulose in terms of crystallinity, chemical purity, tensile strength, elastic modulus, water absorption and biocompatibility, and is considered to be a kind of excellent performance Novel natural bionanomaterials. In recent years, the research on bacterial cellulose has become one of the hotspots in the field of microbial synthetic materials, and has shown great application potential in the fields of biomedicine, tissue engineering scaffold materials, acoustic equipment, food, cosmetics, and papermaking. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P19/04C12R1/01
CPCC12N1/02C12N1/20C12P19/04C12R2001/01C12N1/205
Inventor 胡建颖吴佳婧王昕怡黄婕高红亮牛延宁易正芳常忠义
Owner EAST CHINA NORMAL UNIV
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