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Immunopotentiator for swine fever live vaccine and preparation method thereof

A technology of immune enhancer and swine fever live vaccine, which is applied in the field of veterinary biological products, can solve the problems of low vaccine immunization success rate, low immune function of the body, and insufficient immune stimulation, so as to shorten the window period, avoid latent infection, The effect of prolonged immunity

Active Publication Date: 2021-01-08
SHANGHAI CHUANG HONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are currently three types of vaccines for preventing swine fever on the market: milk rabbit vaccine, cell vaccine and lymphocytic vaccine. These three are live vaccines, which have certain efficacy in clinical use, but the vaccine’s immunization success rate is not high. In addition to factors such as vaccine quality, immunization procedures, and inoculation sites, low immune function and insufficient immune stimulation are important factors. Some pathogenic infections lead to low immune function or immunosuppression of the body.

Method used

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  • Immunopotentiator for swine fever live vaccine and preparation method thereof
  • Immunopotentiator for swine fever live vaccine and preparation method thereof
  • Immunopotentiator for swine fever live vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Liquid preparation containing Corynebacterium brevis preparation (CPP): Take 25ml Tween-80 concentration of 100μg / ml aqueous solution, add 30μg astragalus polysaccharide under slow stirring, 30μg edetate disodium salt, 3μg poly Inosinic acid (PolyI:C), 3 μg of manganese gluconate, 3 μg of potassium iodide was added after stirring for 5 min, and breve-shaped bacteria (CP), Tween-80 in the liquid containing breve-shaped bacteria preparation (CPP) Content: EDTA disodium salt and polyinosinic acid (PolyI:C) content=75, the mass ratio of described Tween-80 and potassium iodide is 750:1, then continue to stir to obtain golden yellow after 20min The liquid containing Corynebacterium brevis preparation (CPP), sealed in a brown collection bottle, filled with liquid nitrogen for aseptic preservation, the shelf life is 2 years;

[0024] B, the preparation of immunopotentiator: get the liquid containing the brevis preparation (CPP) of appropriate measure and be placed in the rea...

Embodiment 2

[0026] a. Preparation of liquid containing Corynebacterium brevis: take 25ml of Tween-80 aqueous solution with a concentration of 100μg / ml, add 150μg of astragalus polysaccharide, 60μg of ethylenediaminetetraacetic acid disodium salt, and 30μg of polyinosinic acid under slow stirring (PolyI:C), 30 μg of manganese gluconate, continue to stir for 5min and add 30 μg of potassium iodide, and add breve bacillus (CPP), the Tween-80 content in the liquid containing breve bacillus preparation (CPP): ethylene dichloride The content of amine tetraacetic acid disodium salt and polyinosinic acid (PolyI:C)=30, the mass ratio of Tween-80 and potassium iodide is 85:1, and then continue stirring for 40min to obtain golden yellow containing short coryneform bacteria The liquid of the preparation (CPP) is packaged in a brown collection bottle, filled with liquid nitrogen for sterile storage, and has a shelf life of 2 years;

[0027] B, the preparation of immunopotentiator: get the liquid that c...

Embodiment 3

[0029] a. Preparation of liquid containing Corynebacterium brevis: take 25ml of an aqueous solution with a concentration of 100 μg / ml of Corynebacterium brevis, add 75 μg of astragalus polysaccharide, 45 μg of ethylenediaminetetraacetic acid disodium salt, and 15 μg of polyinosinic acid under slow stirring (PolyI:C), 15 μg of manganese gluconate, continue to stir for 6 minutes and add 15 μg of potassium iodide, and add breve-shaped bacteria (CPP), the content of Tween-80 in the liquid containing breve-shaped bacteria: ethylenediaminetetraacetic acid di The content of sodium salt and polyinosinic acid (PolyI:C)=42, the mass ratio of described brevis and potassium iodide is 150:1, and then continue to stir for 40min to obtain golden yellow liquid containing brevis, package In a brown collection bottle, filled with liquid nitrogen for sterile storage, the shelf life is 2 years;

[0030] B, the preparation of immunopotentiator: take the liquid containing brevus of appropriate meas...

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Abstract

The invention discloses an immunopotentiator for a swine fever live vaccine. The immunopotentiator comprises liquid containing corynebacterium parvum (CP) immune particles and solvent,the corynebacterium parvum particle liquid is mainly composed of Tween-80,astragalus polysaccharide,poly I:C (Poly I:C),ethylene diamine tetraacetic acid,manganese gluconate,potassium iodide and corynebacterium parvum preparation (CPP) according to suitable proportions and medicine compatibility. Compared with the prior art,the prepared immunopotentiator for the swine fever live vaccine can effectively inhibit construction and re-activation of swine fever virus latent infection,generate short-term immunity through induction,improve the immune effect of the swine fever vaccine,prolong the immune duration period,effectively avoid the latent infection of the swine fever virus,provide guarantee for prevention and eradication of the swine fever,be widely applied to prevention and treatment of swine fever in pig farms,and improve the economic benefits of the pig farms.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an immune enhancer for swine fever live vaccine and a preparation method thereof. Background technique [0002] Adjuvants, also known as immunopotentiators or immunomodulators, refer to a class of substances that can promote or enhance the body's specific immune response when used together with vaccines. Its main function is to enhance the response intensity and persistence of the body's immune system (humoral and cellular immune systems). Adjuvants have been used to enhance the immune effect of vaccines for more than 80 years. Therefore, the study of adjuvants has become an important content in the field of immunology. [0003] Classical swine fever (CSF) is caused by classical swine fever virus (CSFV), an acute septic and contagious infectious disease with extremely high mortality. The pig industry is extremely dangerous. The most effectiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/187A61P31/14A61P37/04
CPCA61K39/12A61K39/39A61K2039/5254A61K2039/552A61K2039/55561A61K2039/55583A61K2039/55594A61P31/14A61P37/04C12N2770/24334
Inventor 赖志马晶晶韩相敏高俊锋吴碧清
Owner SHANGHAI CHUANG HONG BIOTECH
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