Immunopotentiator for porcine atrophic rhinitis inactivated vaccine and preparation method thereof

An immune enhancer, a technology for atrophic rhinitis, which is applied in the field of immune enhancer for swine atrophic rhinitis inactivated vaccine and the field of preparation thereof, can solve the problems of long duration of immunization, expensive raw materials, poor effect and the like, and achieves the duration of immunization The effect of prolonging, avoiding latent infection and improving economic efficiency

Active Publication Date: 2020-12-01
SHANGHAI CHUANG HONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adjuvants of porcine atrophic rhinitis inactivated vaccine currently on the market include aluminum hydroxide gel, lipid adjuvant and propolis adjuvant; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but it cannot induce cellular immunity ; Lipid adjuvant and propolis adjuvant have good immunogenicity, can induce humoral immunity and cellular immunity, and have a long duration of immunity, but the raw materials are expensive and the cost is too high
Therefore, in recent years, researchers from various countries have developed a series of new adjuvants using different raw materials and methods, but none of them have achieved satisfactory results due to poor effects or high toxicity.

Method used

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  • Immunopotentiator for porcine atrophic rhinitis inactivated vaccine and preparation method thereof
  • Immunopotentiator for porcine atrophic rhinitis inactivated vaccine and preparation method thereof
  • Immunopotentiator for porcine atrophic rhinitis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Preparation of liquid containing dry powder immune particles: take 30ml of an aqueous solution with an aluminum hydroxide concentration of 100ug / ml, add 4ug of ginsenoside, 4ug of cholesterol, and 4ug of diethylaminoethyl dextran under slow stirring, and continue to stir Add polyethylene glycol after 5min, the total moles of vitamin C and potassium iodide in the liquid containing dry powder immune particles / aluminum hydroxide moles=4, and the mass ratio of the aluminum hydroxide to polyethylene glycol is 1:1 , and then continue stirring for 20 min to obtain a golden yellow liquid containing dry powder immune particles. Observed by atomic microscope, the diameter of the obtained dry powder immune particles is 50 nm. The liquid containing dry powder immune particles is encapsulated in a brown collection bottle and filled with liquid nitrogen. Do aseptic preservation, the shelf life is 2 years;

[0023] b, the preparation of immune enhancer: get a suitable amount of liq...

Embodiment 2

[0025] a. Preparation of liquid containing dry powder immune particles: Take 30ml of an aqueous solution with a concentration of 100ug / ml of aluminum hydroxide, add 80ug of ginsenoside, 80ug of cholesterol, and 40ug of diethylaminoethyl dextran under slow stirring, and continue to stir Add polyethylene glycol after 3 minutes, the total moles of vitamin C and potassium iodide in the liquid containing the dry powder immune particles / aluminum hydroxide moles=1, and the mass ratio of the aluminum hydroxide to polyethylene glycol is 1:0.1 , and then continue stirring for 10 min to obtain a golden yellow liquid containing dry powder immune particles. Observed by atomic microscope, the diameter of the obtained dry powder immune particles is 120 nm. The liquid containing dry powder immune particles is encapsulated in a brown collection bottle and filled with liquid nitrogen. Do aseptic preservation, the shelf life is 2 years;

[0026] b, the preparation of immune enhancer: get a suita...

Embodiment 3

[0028] a. Preparation of liquid containing dry powder immune particles: take 30ml of an aqueous solution with a concentration of 100ug / ml of aluminum hydroxide, add 40ug of ginsenoside, 40ug of cholesterol and 20ug of diethylaminoethyl dextran under slow stirring, and continue to stir for 4min After adding polyethylene glycol, the total number of moles of vitamin C and potassium iodide in the liquid containing the dry powder immune particles / the number of moles of aluminum hydroxide=2, and the mass ratio of the aluminum hydroxide to polyethylene glycol is 1:0.5, Continue stirring for 15 minutes to obtain a golden yellow liquid containing dry powder immune particles, observe with an atomic microscope, and the diameter of the obtained dry powder immune particles is 100 nm. The liquid containing dry powder immune particles is encapsulated in a brown collection bottle and filled with liquid nitrogen. Sterile preservation, the shelf life is 2 years;

[0029] b, the preparation of i...

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Abstract

The invention discloses an immune enhancer for porcine atrophic rhinitis inactivated vaccine, which comprises a liquid containing dry powder immune particles and a solvent, and the sub-liquid containing dry powder immune particles mainly consists of aluminum hydroxide aqueous solution, ginsenoside, cholesterol and vitamin C , diethylaminoethyl dextran, potassium iodide, and polyethylene glycol in a suitable proportion. Compared with the prior art, the immunopotentiator for porcine atrophic rhinitis inactivated vaccine prepared by the present invention can effectively inhibit the establishment and reactivation of latent infections of Bordetella bronchiseptica and Pasteurella multocida, and can shorten the The window period of antibody production improves the immune effect of porcine atrophic rhinitis vaccine, prolongs the duration of immunity, effectively avoids the latent infection of porcine atrophic rhinitis, provides a guarantee for the prevention and eradication of porcine atrophic rhinitis disease, and can be widely used It is used in pig farms to prevent and treat swine atrophic rhinitis and improve the economic benefits of pig farms.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an immune enhancer for porcine atrophic rhinitis inactivated vaccine and a preparation method thereof. Background technique [0002] Porcine atrophic rhinitis is a chronic and infectious respiratory disease mainly caused by Bordetella bronchiseptica and toxin-producing Pasteurella multocida. The World Health Organization defines it as a category B disease. The sick pigs have adverse reactions such as sneezing, nosebleeds, facial deformation, nasal skewness and growth retardation, resulting in reduced feed conversion rate of pigs, low production performance, and intensive feeding. The chemical pig industry has caused huge economic losses. At the same time, after the pathogen infects the pig, it damages the normal structure and function of the respiratory tract, reduces the resistance of the pig body, and is easily infected with other pathogens, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/116A61K39/102A61K39/02A61P31/04A61P37/04
CPCA61K39/0208A61K39/102A61K39/39A61K2039/521A61K2039/552A61K2039/55505A61K2039/55511A61K2039/55577A61K2039/55583A61K2039/57A61K2039/575
Inventor 马晶晶赖志吴碧清高俊锋韩相敏张秉金沈明志张瑜
Owner SHANGHAI CHUANG HONG BIOTECH
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