Method for simultaneously extracting hypocrellin A and Elsinochrome A from solid fermented material of Shiraia bambusicola

Abstract

The invention discloses a method for simultaneously extracting hypocrellin A and Elsinochrome A from a solid fermented material of Shiraia bambusicola. The method comprises the following steps: (1) leaching the solid fermented material of Shiraia bambusicola separately by using organic solvents with different polarities from weak to strong according to the polarity; (2) concentrating extract intoextract paste, and performing sequential elution in a chromatography column by using organic solutions with gradient ratios separately so as to elute the two substances of hypocrellin A and Elsinochrome A from the same column in batches; and (3) performing identification, concentration and crystallization on the obtained chromatographic liquid so as to obtain the hypocrellin A and Elsinochrome A.By leaching the solid fermented material of Shiraia bambusicola through an organic solvent gradient method, simultaneous acquirement of the hypocrellin A and Elsinochrome A is achieved in the chromatography process of one chromatography column, meanwhile, a high extraction rate and high purity are achieved, and the organic solvents can be recycled without waste discharge; the extraction method isbeneficial to industrial production of hypocrellin A and Elsinochrome A.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for efficiently extracting hypocretin A and coelomycin A in bamboo yellow fungus fermentation materials. Background of the invention [0002] Bamboo yellow is a traditional medicinal fungus in my country. It has the functions of relieving cough and pain, relaxing tendons and activating collaterals, dispelling wind and dampness, nourishing the middle and replenishing qi, promoting blood circulation, dissipating blood stasis and stimulating menstruation. The content of hypocrellin and hypocretin in the secondary metabolites of bamboo yellow is relatively high. Coelomycins include coelomycin A, coelomycin B, coelomycin C, and coelomycin D, all of which belong to perylenequinone compounds. As good photosensitizers, perylenequinones have different degrees of anticancer activity and antiviral activity (Hudson J B, Imperial V, Haugland R P, et al. Photochemi...

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Problems solved by technology

[0005] For Hypocretin A crystals, Yin Zhiqi and others first used highly polar ethanol in 15kg of Zhuhuang decoction pieces, and then extracted with ethyl acetate to obtain 65mg Hypocretin A crystals. The purity is not disclosed, but the inventor of this patent The experiment found that only using ethanol reflux to extract the extract, the extraction rate is low, and the elution steps are complicated by using petroleum ether, acetone, ethyl acetate ratio silica gel column, and the extraction rate is also low; a kind of invention invented by Yan Jiading et al. The preparation method of high-purity hypocrellin A uses natural bamboo yellow Chinese herbal medicines and does not disclose the crystallization purity of hypocrellin A and only uses ethanol solvent. There is no clear extraction rate and no separation using the polarity of organic solvents.

For the first time, Zheng Lixiong and others used 4.4kg bamboo red fungus crude powder to obtain about 74mg of coelomycin A crystals with acetone, ethanol, cyclohexane, ethyl acetate organic solvents and silica gel chromatography column, the purity of which was not determined. It is low and uses more solvents and is more complicated. It is extracted from the coarse powder of bamboo red fungus medicinal materials, and the raw materials are not easy to obtain, and only one kind of substance crystallization is obtained.