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A kind of fermentation medium of Bacillus licheniformis and production method thereof

A technology of Bacillus licheniformis and fermentation medium, applied in the field of microorganisms, can solve the problems of high cost, low bacterial content and low spore count in fermentation liquid, and achieve the effects of promoting growth, facilitating large-scale industrial production, and shortening the fermentation cycle

Active Publication Date: 2020-12-01
GUANGDONG RONGDA BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing fermentation medium of Bacillus licheniformis has the problems of low bacterial content and spore count in the fermentation broth, and high cost.

Method used

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  • A kind of fermentation medium of Bacillus licheniformis and production method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, a kind of fermentation medium of bacillus licheniformis

[0028] The fermentation medium of the bacillus licheniformis consists of the following components and their contents:

[0029] Sucrose 30g / L, sodium gluconate 10g / L, peptone 8g / L, yeast powder 5g / L, ammonium sulfate 0.5g / L, zinc sulfate 0.2g / L, potassium dihydrogen phosphate 1g / L, calcium chloride 0.1g / L, growth promoter 0.02g / L; the growth promoter consists of tyrosine and sorbic acid in a weight ratio of 5:3.

Embodiment 2

[0030] Embodiment 2, a kind of fermentation medium of bacillus licheniformis

[0031] The fermentation medium of the bacillus licheniformis consists of the following components and their contents:

[0032] Sucrose 38g / L, sodium gluconate 20g / L, peptone 15g / L, yeast powder 8g / L, ammonium sulfate 1g / L, zinc sulfate 0.6g / L, potassium dihydrogen phosphate 2g / L, calcium chloride 0.5g / L L, growth promoter 0.08g / L; the growth promoter is composed of tyrosine and sorbic acid in a weight ratio of 8:1.

Embodiment 3

[0033] Embodiment 3, a kind of fermentation medium of bacillus licheniformis

[0034] The fermentation medium of the bacillus licheniformis consists of the following components and their contents:

[0035] Sucrose 36g / L, sodium gluconate 15g / L, peptone 12g / L, yeast powder 6g / L, ammonium sulfate 0.8g / L, zinc sulfate 0.5g / L, potassium dihydrogen phosphate 1.2g / L, calcium chloride 0.3 g / L, growth promoter 0.06g / L; the growth promoter consists of tyrosine and sorbic acid in a weight ratio of 7:2.

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Abstract

The invention relates to the technical field of microbial technologies, in particular to a fermentation medium of bacillus licheniformis and a production method thereof. The fermentation medium of bacillus licheniformis is prepared from the following ingredients, in content: 30-38g / L of saccharose, 10-20g / L of sodium gluconate, 8-15g / L of peptone, 5-8g / L of yeast powder, 0.5-1g / L of ammonium sulfate, 0.2-0.6g / L of zinc sulfate, 1-2g / L of monopotassium phosphate, 0.1-0.5g / L of calcium chloride and 0.02-0.08g / L of growth promoter. The fermentation medium of bacillus licheniformis provided by theinvention can not only promote bacillus licheniformis to enter into the logarithmic phase rapidly, but also can prolong the stable phase of bacillus licheniformis, and the high-content viable countsand spore counts can be obtained through fermentation. The fermentation medium can greatly shorten the fermentation period, lowers the production cost, and is more beneficial to large-scale industrialized production.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a fermentation medium of bacillus licheniformis and a production method thereof. Background technique [0002] Bacillus licheniformis, belonging to the genus Bacillus, is a Gram-positive bacterium that is widely distributed in nature and is a dominant species in soil and plant microecology. It has a strong inhibitory effect on a variety of plant pathogens, such as Fusarium, sclerotinia, rice blast fungus and rice sheath blight fungus, etc., showing good biocontrol potential. In the field control effect test on tomato gray mold, Bacillus licheniformis is equivalent to the chemical agent procymidone, which can reach more than 60%; it also has the ability to colonize and compete on the surface of tomato plants and induce systemic resistance in plants ability; it can produce a variety of antibiotic substances, and its antibacterial protein can inhibit apple ringworm and anthr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/10
CPCC12N1/20C12N1/38
Inventor 吴阳开刘宗新张泳林万华黄炜乾方文棋
Owner GUANGDONG RONGDA BIOENG CO LTD
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