Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for preparing chlorogenic acid monomer from black diamond potato

A technology for chlorogenic acid and potato is applied in the field of preparing chlorogenic acid monomers, which can solve the problems of low purity of chlorogenic acid, high requirements, inability to meet functional research, etc., and achieves easy control of technological process, simple extraction method and raw material source. wide effect

Active Publication Date: 2021-05-04
HUBEI NORMAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report of extracting chlorogenic acid from black diamond potatoes, and the spray drying unit needs to be used in the first invention, which has high requirements for equipment, and the purity of extracting chlorogenic acid is low, only 70.3%. Satisfy the functional research of quantitative standard and chlorogenic acid; in the latter two inventions, enzymatic hydrolysis method is required, and enzyme and enzymatic hydrolysis products are introduced in the process of enzymatic hydrolysis, which increases the difficulty of separation and purification, and the extracted green The purity of raw acid is still not high enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing chlorogenic acid monomer from black diamond potato
  • A method for preparing chlorogenic acid monomer from black diamond potato
  • A method for preparing chlorogenic acid monomer from black diamond potato

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 of the present invention provides a kind of method for preparing chlorogenic acid monomer from black diamond potato, comprises the steps:

[0023] (1) Extraction: Wash and peel the non-rotten Heijingang potatoes, then cut the potatoes into strips, dry the potato strips at 50°C, grind them after drying, and pass through a 40-mesh sieve to obtain Heijingang potato powder ;Add 2g of Heijingang potato powder into 120mL hydrochloric acid solution with a concentration of 0.3mol / L, and then extract it at 70°C for 2 hours, then transfer the extract solution to a centrifuge, and centrifuge at a speed of 8000r / min. Separation for 10 minutes, the obtained supernatant is the chlorogenic acid extract;

[0024] (2) Prepare crude chlorogenic acid powder: pass the chlorogenic acid extract solution obtained in step (1) through an adsorption column filled with macroporous resin D101 type at a flow rate of 1 mL / min, after dynamic adsorption is saturated, use Wash the macropo...

Embodiment 2

[0036] Embodiment 2 of the present invention provides a kind of method for preparing chlorogenic acid monomer from black diamond potato, comprises the steps:

[0037] (1) Extraction: Wash and peel the non-rotten Heijingang potatoes, then cut the potatoes into strips, dry the potato strips at 50°C, grind them after drying, and pass through a 40-mesh sieve to obtain Heijingang potato powder Add 2g of black diamond potato powder into 160mL of hydrochloric acid solution with a concentration of 0.1mol / L, and then leaching at 70°C for 1 hour, then transfer the leaching solution into a centrifuge, at a speed of 8000r / min, Centrifuge for 10 minutes, and the obtained supernatant is the chlorogenic acid extract;

[0038] (2) Make crude chlorogenic acid powder: the chlorogenic acid extract obtained in step (1) is passed through an adsorption column filled with macroporous resin AB-8 type at a flow rate of 1 mL / min, after dynamic adsorption is saturated, Wash the macroporous resin adsorp...

Embodiment 3

[0042] Embodiment 3 of the present invention provides a kind of method for preparing chlorogenic acid monomer from black diamond potato, comprises the steps:

[0043] (1) Extraction: Wash and peel the non-rotten Heijingang potatoes, then cut the potatoes into strips, dry the potato strips at 40°C, grind them after drying, and pass through a 80-mesh sieve to obtain Heijingang potato powder Add 20g of Heijingang potato powder into 1200mL concentration of 2wt% citric acid solution, then extract at 60°C for 2h, then transfer the extract into a centrifuge, and centrifuge at a speed of 6000r / min Separation for 10 minutes, the obtained supernatant is the chlorogenic acid extract;

[0044] (2) Make crude chlorogenic acid powder: the chlorogenic acid extract obtained in step (1) is passed through an adsorption column filled with macroporous resin X-5 type at a flow rate of 1 mL / min, after dynamic adsorption is saturated, Wash the macroporous resin adsorption column with deionized wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing chlorogenic acid monomer from black diamond potato, which comprises the following steps: (1) adding extract liquid to black diamond potato powder to prepare chlorogenic acid extract; The original acid extract is passed through a macroporous resin adsorption column at a flow rate of 1-3 mL / min, and after washing with water and eluting with ethanol, the crude chlorogenic acid is obtained; (3) the crude chlorogenic acid is prepared into the first chlorogenic acid solution, And extract the first chlorogenic acid solution with ethyl acetate for 1 to 2 times, collect the ethyl acetate layer, concentrate and dry to obtain the fine chlorogenic acid; (4) prepare the fine chlorogenic acid into the second chlorogenic acid solution, and use half The second chlorogenic acid solution is separated by preparative liquid chromatography, and the collected samples are concentrated and dried to obtain chlorogenic acid monomers. The invention extracts chlorogenic acid from black diamond potatoes through solvent extraction, ethyl acetate extraction and semi-preparative high-performance liquid chromatography, and the purity of the prepared chlorogenic acid monomer reaches 97-99%.

Description

technical field [0001] The invention belongs to the technical field of preparing chlorogenic acid monomers, and in particular relates to a method for preparing chlorogenic acid monomers from black diamond potatoes. Background technique [0002] Chlorogenic acid is a kind of organic acid containing hydroxyl and o-diphenolic hydroxyl. Its chemical name is 5-O-caffeoylquinic acid. It is a kind of phenylpropanoid produced by plants through shikimic acid pathway during aerobic respiration. class of compounds. Chlorogenic acid is an important biologically active substance with a wide range of pharmacological effects and biological activities. It is not only the main active ingredient of many medicinal materials and Chinese patent medicines for antibacterial detoxification, anti-inflammatory and choleretic, but also has anti-virus, white blood cell increase, anti-tumor, Lowering blood pressure, lowering blood lipids, scavenging free radicals and stimulating the central nervous sys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56C07C67/58
CPCC07C67/48C07C67/56C07C67/58C07C2601/14C07C69/732
Inventor 侯建军刘细霞张雪莉曾丽君马秋平李雅妮侯垚瑶陆琪陈思锐
Owner HUBEI NORMAL UNIV