The Application of Nucleus Penicillin as Agricultural Fungicide

A kind of nuclear complex penicillin, agricultural technology, applied in the field of agriculture

Active Publication Date: 2021-11-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The natural product Sclerotiorin compound described by the present invention has no report on the activity of agricultural pathogenic bacteria, although the master's thesis "Synthesis and bactericidal activity research of Sclerotiorin derivatives" discloses the antibacterial activity of compound Sclerotiorin derivatives; , but the agricultural pathogenic bacteria studied in the present invention are not the same as the pathogenic bacteria in the paper
The activity of the compound of the present invention on the studied agricultural pathogenic bacteria is not only obviously stronger than the activity reported in the literature, but also the compound of the present invention is stable in structure, without the shortcomings of the compounds reported in the literature such as light instability, acid-base sensitivity, etc.

Method used

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  • The Application of Nucleus Penicillin as Agricultural Fungicide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Endophytic fungi Penicillium sp. (TA33-1) culture

[0017] The medium used for the culture of the strain contains 1.0% glucose (percentage by weight, the same below), 0.2% yeast extract, 0.2% peptone, 1.0% agar, 3.0% sodium chloride, and the rest is water. Strains were cultured at 30°C for 3 days.

[0018] (2) Endophytic fungi Penicillium Fermentation of sp. (TA33-1)

[0019] The used substratum of fermentation culture contains rice 40.0% (percentage by weight, the same below), sodium chloride 3.0%, and the rest is water; Fungal bacterial strains were cultivated for 90 days at 28°C.

[0020] (3) extraction and separation of formula I compound

[0021]Get 60 bottles of the mycelium obtained in step (2), extract 3 times with chloroform-methanol mixture (1:1), concentrate under reduced pressure, and extract 3 times with ethyl acetate to obtain crude extract; The extracts were subjected to normal phase silica gel column chromatography (stationary phase is 200-30...

Embodiment 2

[0026] The bactericidal activity test of the formula I compound of the present invention is tested according to the following references test methods: Thiyagarajan, V.; Harder, T.; Qiu, J. W.; Qian, P. Y. Mar Biol (Berl) 2003,143, 543–554. Liu, Q. A.; Shao, C. L.; Gu, Y., C.; Blum, M.; Gan, L. S.;Wang, K. L.; Chen, M.; Wang, C. Y. J. Agric. Food Chem. 2014, 62, 3183–3191.

[0027] Test result shows, when the compound of formula I of the present invention is 20 ppm, to melon anthracnose Glomerella lagenarium , Septoria tritici Septoria tritici , Glycystium husk Gaeumannomyces graminis , wheat snow mold leaf blight Monographella nivalis , Arachis coccus Mycosphaerella arachidis and Sclerotinia Sclerotinia sclerotiorum The bacteriostasis rate of the present invention is 100%, 100%, 100%, 100%, 50% and 50%, respectively, when the concentration is 200 ppm, the compound of formula I of the present invention is effective against downy mildew Plasmopara viticola The...

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Abstract

The invention provides an agricultural fungicide, which is characterized in that it uses sclerotin or its salt as an active ingredient for preventing and treating sclerotinia Sclerotinia sclerotiorum , wheat snow mold leaf blight Monographella nivalis , Glycystia husk Gaeumannomyces graminis , Septoria tritici Septoria tritici , melon anthracnose Glomerella lagenarium , Peanut Mycosphaerella arachidis and downy mildew Plasmopara viticola caused by agricultural diseases.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to the application of a sclerotin compound as an agricultural fungicide. Background technique [0002] Fungal pathogenic diseases are one of the natural disasters that seriously endanger agricultural production and cause huge losses to agricultural production. The average annual output of crops in the world is reduced by about 500 Mt. Fungal pathogenic diseases can not only cause crop yield reduction, but also seriously threaten the quality and safety of agricultural products and their international trade. For example, Sclerotinia ( Sclerotinia sclerotiorum ) is a worldwide important plant pathogen that harms crops and vegetables. It can widely infect many monocotyledonous and dicotyledonous plants; wheat snow mold leaf blight ( Monographella nivalis ) can be harmful from seedling to filling stage, causing symptoms such as bud rot, leaf withering, sheath rot and ear rot, wherein lea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/76A01N43/16A01P3/00
CPCA01N43/16C07D311/76
Inventor 魏美燕邵长伦王长云王翠芳
Owner OCEAN UNIV OF CHINA
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