The Application of Nucleus Penicillin as Agricultural Fungicide
A kind of nuclear complex penicillin, agricultural technology, applied in the field of agriculture
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Embodiment 1
[0016] (1) Endophytic fungi Penicillium sp. (TA33-1) culture
[0017] The medium used for the culture of the strain contains 1.0% glucose (percentage by weight, the same below), 0.2% yeast extract, 0.2% peptone, 1.0% agar, 3.0% sodium chloride, and the rest is water. Strains were cultured at 30°C for 3 days.
[0018] (2) Endophytic fungi Penicillium Fermentation of sp. (TA33-1)
[0019] The used substratum of fermentation culture contains rice 40.0% (percentage by weight, the same below), sodium chloride 3.0%, and the rest is water; Fungal bacterial strains were cultivated for 90 days at 28°C.
[0020] (3) extraction and separation of formula I compound
[0021]Get 60 bottles of the mycelium obtained in step (2), extract 3 times with chloroform-methanol mixture (1:1), concentrate under reduced pressure, and extract 3 times with ethyl acetate to obtain crude extract; The extracts were subjected to normal phase silica gel column chromatography (stationary phase is 200-30...
Embodiment 2
[0026] The bactericidal activity test of the formula I compound of the present invention is tested according to the following references test methods: Thiyagarajan, V.; Harder, T.; Qiu, J. W.; Qian, P. Y. Mar Biol (Berl) 2003,143, 543–554. Liu, Q. A.; Shao, C. L.; Gu, Y., C.; Blum, M.; Gan, L. S.;Wang, K. L.; Chen, M.; Wang, C. Y. J. Agric. Food Chem. 2014, 62, 3183–3191.
[0027] Test result shows, when the compound of formula I of the present invention is 20 ppm, to melon anthracnose Glomerella lagenarium , Septoria tritici Septoria tritici , Glycystium husk Gaeumannomyces graminis , wheat snow mold leaf blight Monographella nivalis , Arachis coccus Mycosphaerella arachidis and Sclerotinia Sclerotinia sclerotiorum The bacteriostasis rate of the present invention is 100%, 100%, 100%, 100%, 50% and 50%, respectively, when the concentration is 200 ppm, the compound of formula I of the present invention is effective against downy mildew Plasmopara viticola The...
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