Application of Bupleuri Radix Extract as Feed Additive for Grouper

A Bupleurum extract and feed additive technology, applied in the application field of grouper feed additives, can solve problems such as abuse, insufficient disease prevention and control, deterioration, etc., achieve no toxic side effects, improve feed efficiency and immunity, and increase growth performance effect

Active Publication Date: 2021-11-09
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the expansion of breeding scale, the deterioration of water environment, insufficient disease prevention and control, and the abuse of chemical substances such as antibiotics, frequent outbreaks of grouper liver disease have resulted in a large number of fish deaths and economic losses.

Method used

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  • Application of Bupleuri Radix Extract as Feed Additive for Grouper
  • Application of Bupleuri Radix Extract as Feed Additive for Grouper
  • Application of Bupleuri Radix Extract as Feed Additive for Grouper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In order to explore the effect of Bupleurum extract (20:1) on the growth performance of the pearl gentian grouper in the feed, add Bupleurum extract (20:1) to the basic feed to feed the pearl gentian grouper, set 5 gradients, and add the concentration respectively 0, 0.02%, 0.04%, 0.08%, 0.16%, and the experimental breeding period was 8 weeks.

[0039] The composition of table 1 feed and nutrient composition table (%)

[0040]

[0041] Table 2 Bupleurum extract on hybrid grouper whole fish and muscle nutritional components (%)

[0042]

[0043] The result is as figure 1 As shown in Table 2, when Bupleurum extract was fed to pearl gentian grouper for eight weeks, the weight gain rate (WG) of the test fish between different treatment groups had a significant difference, showing a trend of first increasing and then decreasing, and all in the grouper of Bupleurum gentian The maximum value was reached when the addition of Hu extract was 0.08%.

[0044] Feed efficien...

Embodiment 2

[0048] In order to explore the effect of Bupleurum chinensis extract on the liver morphology and histochemistry of pearl gentian grouper. Add Bupleurum extract (the extraction ratio is 20:1) to the basic feed, set 5 gradients, and add the concentration to be 0, 0.02%, 0.04%, 0.08%, 0.16% (as shown in Table 1), and breed Eight weeks later, liver tissues were taken for HE staining and Oil Red O staining.

[0049] The result is as figure 2 and image 3 The results show that adding 0.04-0.08% Bupleurum extract to the diet can significantly improve the liver structure of the hybrid grouper. The nuclei of liver cells in the unsupplemented group are shifted and have a large number of vacuoles. With the increase of the concentration of Bupleurum extract, the nucleus The offset and vacuoles were significantly improved; it was observed by Oil Red O staining that with the increase of Bupleurum concentration, the number of lipid droplets in the liver was significantly reduced, and the ...

Embodiment 3

[0052] In order to explore the effect of adding Bupleurum radicis extract on the liver fat metabolism-related genes of pearl gentian grouper. Add Bupleurum extract (the extraction ratio is 20:1) to the basic feed, set 5 gradients, and add the concentration to be 0, 0.02%, 0.04%, 0.08%, 0.16% (as shown in Table 1), and breed After 8 weeks, the liver tissue was taken for fluorescence quantitative analysis.

[0053] The result is as Figure 4 It was shown that adding 0.08% Bupleurum extract to feed could activate the expression of hybrid grouper lipid metabolism gene PPARα, regulate downstream genes related to fatty acid β oxidation, fatty acyl-CoA oxidase, carnitine palmitoyltransferase Ⅰ, etc. expression, thereby accelerating the decomposition of fatty acids. Adding 0.04-0.08% Bupleurum extract to the feed can significantly down-regulate the expression of LXRα, which can inhibit some key enzyme genes of fatty acid production, such as acetyl CoA carboxylase and fatty acid synt...

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Abstract

The invention discloses the application of Bupleurum radix extract as a grouper feed additive. As a feed additive, Bupleurum extract can improve the morphology and structure of grouper liver, reduce liver fat content and improve immunity. Specifically, the Bupleurum radix extract is added to the basal feed of the grouper, and according to the growth performance index, the appropriate addition amount is 0.04-0.08% of the basal feed mass. Adding too much will affect the growth performance, liver morphology and fat deposition of grouper. If the amount added is too low, the effect cannot be achieved. Experiments have confirmed that the Bupleurum extract has a special effect on improving the health of the liver of the pearl gentian grouper, and the pearl gentian grouper is a species with a large scale of cultivation at present. The Radix Bupleurum extract of the invention can not only improve the growth performance, feed efficiency, liver fat deposition and immunity of cultured fish, but also has no toxic and side effects and is environmentally friendly.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to the application of Bupleurum bupleuri extract as a grouper feed additive. The Bupleurum chinensis extract can enhance fish immunity, reduce fat deposition, prevent and treat liver damage, and has the effect of protecting the liver. Background technique [0002] At present, due to environmental pollution, the use of high-protein, high-fat or moldy feed, and the abuse of antibiotics and pesticides, fish liver diseases frequently occur in many parts of the country, resulting in large-scale deaths of various fish species. It causes great economic losses to the aquaculture industry. [0003] The liver is the most important metabolic organ and the largest digestive gland in fish. Fish liver disease has become a common and harmful disease in current aquaculture, which has attracted extensive attention. Therefore, the study of feed additives with hepatoprotective effects has impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K10/30A23K10/22A23K10/37A23K10/18A23K20/158A23K20/174A23K20/105A23K20/26
CPCA23K10/18A23K10/22A23K10/30A23K10/37A23K20/105A23K20/158A23K20/174A23K20/26A23K50/80Y02P60/87Y02A40/818
Inventor 叶超霞邹翠云孙珍珠谭小红
Owner SOUTH CHINA NORMAL UNIVERSITY
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