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Essence liquid which is capable of delaying aging, and removing wrinkles, and is prepared based on stem cell technology, and preparation method thereof

A technology of stem cells and essence, which is applied in the fields of pharmaceutical formulations, cosmetic preparations, dressing preparations, etc., can solve problems such as loss of useful components of cells, allergic reactions, and inconvenient use, so as to reduce the probability of allergies and process flow Simple, cost-effective production

Inactive Publication Date: 2018-10-19
珠海市雅莎医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

1. Discarding living stem cells only to obtain cytokines secreted by stem cells is very wasteful from the perspective of resources, such as CN104027794A, CN107184540A and CN106214620A
Living stem cells have more application value, because the stem cell lysate contains many useful anti-aging ingredients such as vascular endothelial growth factor, basic fibroblast growth factor, and epidermal growth factor; Macromolecular proteins and residual cell fragments may produce allergic reactions to the body; 3. The growth factors secreted by stem cells are made into dry freeze powder (such as CN106214620A) and can only be used after dissolving with physiological saline during use, so that users can use it's inconvenient
There are also a few that utilize both the cytokines secreted by stem cells and stem cell lysate, and make these useful ingredients into essence, such as CN106344493A, but the process of making essence in this patent is too complicated, which will lead to excessive loss of useful ingredients in cells. Many, the production cost is too high, and some macromolecular proteins and residual cell fragments are not strictly handled, which is likely to cause allergic reactions to users

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] An anti-aging and anti-wrinkle essence prepared based on stem cell technology and a preparation method thereof, comprising the following steps:

[0025] (1) Commercially purchased human mesenchymal stem cells cryopreserved seed cells;

[0026](2) Cell proliferation culture and passage: The first step is to recover and culture the cells. After taking out the cryopreservation tube from the liquid nitrogen tank, quickly transfer it to a 37°C constant temperature water bath, shake the cryopreservation tube gently, and wait until only one cell remains. When the ice crystals are small, transfer them to a biological safety cabinet, and transfer the completely thawed cell cryopreservation solution to 2-8ml xeno-free human mesenchymal stem cell medium (purchased from Beijing Edes Biotechnology Co., Ltd.), 400g Centrifuge for 0.5min for the first time under certain conditions to remove residual cryoprotectant DMSO and cell debris, resuspend the cells in 0.5-2ml xeno-free human me...

Embodiment 2

[0032] An anti-aging and anti-wrinkle essence prepared based on stem cell technology and a preparation method thereof, comprising the following steps:

[0033] (1) Commercially purchased human mesenchymal stem cells cryopreserved seed cells;

[0034] (2) Cell proliferation culture and passage: The first step is to recover and culture the cells. After taking out the cryopreservation tube from the liquid nitrogen tank, quickly transfer it to a 37°C constant temperature water bath, shake the cryopreservation tube gently, and wait until only one cell remains. When the ice crystals are small, transfer them to a biological safety cabinet, and transfer the completely thawed cell cryopreservation solution to 2-8ml xeno-free human mesenchymal stem cell medium (purchased from Beijing Edes Biotechnology Co., Ltd.), 450g Centrifuge for 1 min for the first time under the conditions to remove residual cryoprotectant DMSO and cell debris, resuspend the cells in 0.5-2ml xeno-free human mesenc...

Embodiment 3

[0040] An anti-aging and anti-wrinkle essence prepared based on stem cell technology and a preparation method thereof, comprising the following steps:

[0041] (1) Commercially purchased human mesenchymal stem cells cryopreserved seed cells;

[0042] (2) Cell proliferation culture and passage: The first step is to recover and culture the cells. After taking out the cryopreservation tube from the liquid nitrogen tank, quickly transfer it to a 37°C constant temperature water bath, shake the cryopreservation tube gently, and wait until only one cell remains. When the ice crystals are small, transfer them to a biological safety cabinet, and transfer the completely thawed cell cryopreservation solution to 2-8ml xeno-free human mesenchymal stem cell culture medium (purchased from Beijing Edes Biotechnology Co., Ltd.), 500g Centrifuge for 2 minutes for the first time under the conditions to remove residual cryoprotectant DMSO and cell debris, resuspend the cells in 0.5-2ml xeno-free ...

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PUM

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Abstract

The invention provides an essence liquid which is capable of delaying aging, and removing wrinkles, and is prepared based on stem cell technology, and a preparation method thereof. The essence liquidwhich is capable of delaying aging, and removing wrinkles, and is prepared based on stem cell technology is prepared through following steps: human mesenchymal stem cell propagation culture and subculture, cell rupturing, semi-permeable membrane dialysis, concentration, and canning. The preparation process is simple; the utilization rate of effective components in growth factors secreted by humanmesenchymal stem cells and stem cell lysate is higher, the product is safer, is capable of promoting skin regeneration, delaying skin cell aging, and preventing wrinkles with better effect, and is convenient to use, and excellent curative effect in the aspect of skin nursing and wrinkle prevention is achieved.

Description

technical field [0001] The invention belongs to the field of skin care and repair, and in particular relates to an anti-aging and wrinkle-removing essence prepared based on stem cell technology and a preparation method thereof. Background technique [0002] The root cause of human aging is the reduction of cell division and differentiation ability, and the aging and death of cells. Relying on one's own adjustment ability cannot slow down one's own aging. However, stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues, organs and human cells, and are called "universal cells" in the medical field. Stem cells can secrete a variety of cytokines, which can effectively regulate collective cell signal transduction, promote cell proliferation and differentiation, replace senescent and dead cells with new cells, activate senescent cells, and delay cell aging and death. [0003] With the development of stem cell technology,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61Q19/08
CPCA61K8/99A61K2800/82A61Q19/08
Inventor 陆贵平钱捷豪钟日英何冰何丽媛
Owner 珠海市雅莎医疗器械有限公司
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