Preparation method of Ag-DMBG/PLLA composite bone support frame with antibacterial functions
A bone scaffold and function technology, applied in the field of artificial bone scaffold materials, can solve the problems of poor anti-infection ability, normal cell damage, and poor dispersion, and achieve the effects of preventing agglomeration, improving dispersion, and promoting biological bonding.
Inactive Publication Date: 2018-10-23
CENT SOUTH UNIV
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Problems solved by technology
[0003] In view of the poor anti-infection ability of the polylactic acid bone scaffold material in the prior art, and the introduction of nano-silver into the polylactic acid bone scaffold material as antibacterial nanoparticles has poor dispersion, easy agglomeration, and damage to normal cells. The purpose of the present invention is to Provides a method for preparing Ag-DMBG / PLLA composite bone scaffold using mesoporous bioglass particles as nano-silver carrier. This method utilizes mesoporous bio-glass particles as nano-silver carrier, which can not only release nano-silver slowly and controlledly, but also achieve long-term antibacterial effect, and the good biological activity of mesoporous bioglass particles can be used to induce the nucleation and growth of bone-like apatite, and improve the biological properties of composite bone scaffolds
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0031] 1) Use an electronic balance to weigh 2g of hydrochloric acid PDA powder and add it to 10mM Tris-HCl buffer to prepare 2.0mgmL -1 PDA solution.
[0032] 2) Use an electronic balance to weigh 5g of MBG, add to the PDA solution prepared above, the solution is magnetically stirred, the main process parameters are: the reaction time is 6h, the magnetic stirring speed is 300r / min; thereafter, the obtained suspension is filtered and separated The precipitate was washed repeatedly with deionized water; DMBG was obtained after drying overnight in a vacuum oven at 60°C.
[0033] 3) Weigh a certain amount of AgNO 3 Crystals, equipped with 60mM AgNO with deionized water 3 solution, and then add a certain amount of DMBG to keep DMBG particles and Ag-containing +The solid-to-liquid ratio between the solutions was 1g / 200ml, and the reaction was performed under magnetic stirring for 12 hours; then the solution was centrifuged to separate the precipitate, and then washed repeatedly ...
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Abstract
The invention discloses a preparation method of an Ag-DMBG / PLLA composite bone support frame with antibacterial functions. The method is characterized in that dopamine is decomposed in pore passages and on surfaces of mesoporous bioactive glass particles to obtain DMBG particles; the DMBG particles are dispersed to a solution containing Ag<+>; through in situ reduction reaction, elemental silver is generated in the pore passages and on the surfaces of the mesoporous bioactive glass particles to obtain Ag-DMBG composite particles; the Ag-DMBG composite particles and L-polylactide powder are subjected to liquid phase mixing and solid-liquid separation; solid is dried and ground; composite powder is obtained; the composite powder is subjected to selective laser sintering to obtain the Ag-DMBG / PLLA composite bone support frame. By using the method, the mesoporous bioactive glass particles are used as nanometer silver carriers; the slow controlled release of nanometer silver can be realized; the long-stage antibacterial effects can be achieved; in addition, good biological activity of the mesoporous bioactive glass particles can be used for inducting osteoid apatite nucleation and growth; the biological performance of the composite bone support frame is improved.
Description
technical field [0001] The invention relates to a composite bone support, in particular to a method for preparing an Ag-DMBG / PLLA composite bone support with antibacterial function, and belongs to the technical field of artificial bone support materials. Background technique [0002] Bacterial infection is one of the most serious complications in bone implant surgery. Traditional treatment methods rely on long-term use of antibiotics, removal surgery, etc., which increase the pain and economic burden of patients, and even lead to disability and death. Polylactic acid (PLLA) is considered to be a promising bone scaffold material because of its good biocompatibility and biodegradability, but PLLA bone scaffolds do not have anti-infection ability, and bacterial infection is prone to occur after implantation in the human body, causing surgery fail. Silver (Ag) and Ag-containing antibacterial materials have attracted widespread attention. Nano-Ag can rapidly increase the concent...
Claims
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IPC IPC(8): A61L27/56A61L27/54A61L27/10A61L27/18
CPCA61L27/10A61L27/18A61L27/54A61L27/56A61L2300/104A61L2300/404A61L2300/602A61L2430/02C08L67/04
Inventor 帅词俊冯佩
Owner CENT SOUTH UNIV