Compound agent for improving heavy metal absorption and transferring capacity of plants and preparation method and application of compound agent
A compound agent and heavy metal technology, which is applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problems of restricting the development and popularization of phytoremediation, low biomass of heavy metal super-accumulated plants, and soil purification. Long time and other issues, to achieve the effect of promoting absorption and transport capacity, facilitating harvesting and enrichment, and simple application methods
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Embodiment 1
[0035] The test materials were cultivated in the light cultivation greenhouse of the laboratory, and the material cultivation and processing were as follows:
[0036] (1) 30 tall fescue (kind is No. 5 hunting dogs, the following examples are the same) seeds are sown in the cave box that length, width and height are respectively 10cm, 10cm, 8cm, the matrix in the cave box is by volume ratio pearl Rock: Vermiculite = 1:1. Under controllable conditions in the greenhouse (day and night temperatures are 24°C and 22°C respectively; light intensity is 300μmol / (m 2 ·s 1 ); the photoperiod is 14 hours of light and 10 hours of darkness), pouring 1 / 2 Hoagland nutrient solution and culturing for nearly 1 month until tall fescue seedlings grow.
[0037] (2) Transfer 12 medium-tall fescue seedlings from (1) into a 250-mL conical flask (wrapped in tinfoil to avoid light) filled with 300 mL 1 / 2 Hoagland nutrient solution and adapt for about 10 days until new roots grow.
[0038] (3) The ne...
Embodiment 2
[0046] Material cultivation and processing Steps (1) and (2) are the same as in Example 1.
[0047] Embodiment 2 carries out under the hydroponic environment of the 1 / 2 Hoagland nutrient solution that initial cadmium concentration is 20mg / L (cadmium solution uses CdCl 2 2.5H 2 (2 preparation), two treatments are set in the test: control group (do not use any hormone) and hormone compound drug treatment (0.5mg / L abscisic acid and 1.5mmol / L salicylic acid compound treatment), each process carries out 4 repetitions, After five days of treatment, the results were averaged. After five days, rinse first, then dry the aboveground part at 60-70°C to constant weight, cut it into pieces, mix evenly, and take 0.3g (when calculating the cadmium content, take the mass after drying to constant weight as the denominator) , respectively add 8mL nitric acid-hydrogen peroxide (obtained by mixing 65wt% nitric acid and 30wt% hydrogen peroxide according to the volume ratio of 3:1) for microwave ...
Embodiment 3
[0052] Material cultivation and processing Steps (1) and (2) are the same as in Example 1.
[0053] Embodiment 3 carries out under the hydroponic environment of the 1 / 2 Hoagland nutrient solution that initial cadmium concentration is 20mg / L (cadmium solution uses CdCl 2 2.5H 2 (2 preparation), two treatments are set in the test: control group (do not use any hormone) and hormone compound drug treatment (0.5mg / L abscisic acid and 0.5mmol / L salicylic acid compound treatment), each process carries out 4 repetitions, After five days of treatment, the results were averaged. After five days, rinse first, then dry the aboveground part at 60-70°C to constant weight, cut it into pieces, mix evenly, and take 0.3g (when calculating the cadmium content, take the mass after drying to constant weight as the denominator) , respectively add 8mL nitric acid-hydrogen peroxide (obtained by mixing 65wt% nitric acid and 30wt% hydrogen peroxide according to the volume ratio of 3:1) for microwave ...
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