Method for producing carotenoid
A technology of carotene and products, applied in the biological field, to achieve the effect of reducing the amount of use, reducing the cost of the enterprise, and cheap raw materials
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Embodiment 1
[0039] A method for producing carotenoids, comprising the steps of:
[0040] Centrifuge the composite microbial culture at 8000rpm and 4°C for 20min, discard the supernatant, collect the bacteria, then add acetone according to the amount of 1kg bacteria: 1L acetone, and then ultrasonically break the wall, the ultrasonic power is 300W, and the time for each ultrasonic The time is 10s, the interval time is 10s, the total ultrasonic time is 30min, then stand still for 30min, then centrifuge at 5000rpm for 5min, collect the supernatant and precipitate, add the same volume of acetone to the precipitate, use ultrasonic treatment for 15min, ultrasonic power is 300W, Each ultrasonic time is 10s, the interval time is 10s, then centrifuged at 5000rpm for 3min, and the supernatant is collected; the two supernatants are combined, and then evaporated to dryness with a rotary evaporator at low temperature, the residue is dissolved with twice the weight of ether, and then added A 30% KOH met...
Embodiment 2
[0049] A method for producing carotenoids, comprising the steps of:
[0050] Centrifuge the complex microbial culture at 8000rpm and 4°C for 20min, discard the supernatant, collect the bacteria, then add acetone according to the amount of 1kg bacteria: 1.5L acetone, and then ultrasonically break the wall, the ultrasonic power is 300W, each ultrasonic The time is 10s, the interval time is 10s, the total ultrasonic time is 30min, then stand still for 30min, then centrifuge at 5000rpm for 5min, collect the supernatant and precipitate, add the same volume of acetone to the precipitate, use ultrasonic treatment for 15min, and the ultrasonic power is 300W , each ultrasonic time is 10s, the interval time is 10s, then centrifuged at 5000rpm for 3min, and the supernatant is collected; the two supernatants are combined, and then evaporated to dryness with a rotary evaporator at low temperature, the residue is dissolved in ether, and then add 6 / 6 A volume of KOH methanol solution with a ...
Embodiment 3
[0059] The changes in the main components of rice straw caused by blasting are shown in Table 1:
[0060] index
[0061] Conclusion: Blasting caused the cell wall of straw to be destroyed, and some hemicellulose and cellulose were degraded and dissolved, which was beneficial to the subsequent enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decreased during the blasting pretreatment process, and hemicellulose passed It is degraded into monosaccharides and oligosaccharides by hydrolysis, which can be used as a carbon source for the strain.
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