Root hair tissue culture rapid seedling-raising method for broussonetia papyrifera
A paper mulberry root and tissue culture technology, applied in the agricultural field, can solve the problems of long seedling raising period and the like, achieve the effects of short seedling raising period, not easy to degenerate, and shortening the tissue culture period
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[0036] The preparation method of the growth-promoting agent is as follows: take the root tissue of the tree, crush it, and carry out biological enzymolysis and fermentation to obtain the active peptide freeze-dried powder, and then prepare a solution with a mass concentration of 15-25%, and add a solution with a mass concentration of 0.1-0.3 % colchicine, 0.5-1.5% indole auxins and 1-3% naphthalene auxins to prepare growth promoters.
[0037] 2) Preparation of soilless nutrient matrix: The soilless nutrient matrix includes the following components in mass content: 8-12% of fermented rapeseed meal powder, 8-12% of fermented tea seed meal powder; 18-22% of hard branch powder of mulberry tree; 55-65% of vermiculite powder; the thickness of soilless nutrient matrix is 5.5-6.5cm. The preparation method is as follows: weighing each component according to the proportion, stirring, mixing, sieving, and high-temperature sterilization at 115-125°C, and then putting the soilless nutrie...
Embodiment 1
[0042] A method for rapidly growing seedlings by tissue culture of mulberry roots, comprising the following steps:
[0043] 1) Cutting the roots: Cut off the branches of the female mulberry tree (the spaceflight hybrid tree species of the Institute of Botany, Chinese Academy of Sciences) about 40cm above the ground, dig up the soil of the female parent tree, and wash the roots , cut the root hairs of φ1-5mm into 3-4cm long under aseptic environment, and then soak them in the growth-promoting agent for 60min. And after cutting off the roots, soak the remaining roots in the growth promoter for 5 minutes, immediately replant and water and apply organic fertilizers for daily weeding.
[0044] The preparation method of the growth-promoting agent is as follows: take the tree root tissue, crush it, and carry out biological enzymatic hydrolysis and fermentation to obtain the active peptide freeze-dried powder, and then prepare a solution with a mass concentration of 20%, and add 0.2% ...
Embodiment 2
[0050] A method for rapidly growing seedlings by tissue culture of mulberry roots, comprising the following steps:
[0051] 1) Cutting the roots and hairs: Cut off the branches of the female mulberry tree (the aerospace hybrid tree species of the Institute of Botany, Chinese Academy of Sciences) at a distance of 35 cm above the ground, dig up the soil of the female plexus tree, and wash the roots. Cut the root hairs of φ1-5mm into 3-4cm length under aseptic environment, and then soak them in the growth-promoting agent for 50 minutes. And after cutting off the roots, soak the remaining roots in the growth promoter for 3 minutes, immediately replant and water and apply organic fertilizers for daily weeding.
[0052] The preparation method of the growth-promoting agent is as follows: take the tree root tissue, crush it, and carry out biological enzymatic hydrolysis and fermentation to obtain the active peptide freeze-dried powder, then prepare a solution with a mass concentration...
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