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Root hair tissue culture rapid seedling-raising method for broussonetia papyrifera

A paper mulberry root and tissue culture technology, applied in the agricultural field, can solve the problems of long seedling raising period and the like, achieve the effects of short seedling raising period, not easy to degenerate, and shortening the tissue culture period

Active Publication Date: 2018-11-16
杭州早稻田生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method regards the tree branch as the tissue culture object, and it just needs the time of at least 26 days from the beginning of tissue culture to transplanting the rooted seedlings carried in the bottle to the greenhouse, and if it is to be planted in the field, it needs at least 2 months or more. Time, long seedling cycle

Method used

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Examples

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Effect test

preparation example Construction

[0036] The preparation method of the growth-promoting agent is as follows: take the root tissue of the tree, crush it, and carry out biological enzymolysis and fermentation to obtain the active peptide freeze-dried powder, and then prepare a solution with a mass concentration of 15-25%, and add a solution with a mass concentration of 0.1-0.3 % colchicine, 0.5-1.5% indole auxins and 1-3% naphthalene auxins to prepare growth promoters.

[0037] 2) Preparation of soilless nutrient matrix: The soilless nutrient matrix includes the following components in mass content: 8-12% of fermented rapeseed meal powder, 8-12% of fermented tea seed meal powder; 18-22% of hard branch powder of mulberry tree; 55-65% of vermiculite powder; the thickness of soilless nutrient matrix is ​​5.5-6.5cm. The preparation method is as follows: weighing each component according to the proportion, stirring, mixing, sieving, and high-temperature sterilization at 115-125°C, and then putting the soilless nutrie...

Embodiment 1

[0042] A method for rapidly growing seedlings by tissue culture of mulberry roots, comprising the following steps:

[0043] 1) Cutting the roots: Cut off the branches of the female mulberry tree (the spaceflight hybrid tree species of the Institute of Botany, Chinese Academy of Sciences) about 40cm above the ground, dig up the soil of the female parent tree, and wash the roots , cut the root hairs of φ1-5mm into 3-4cm long under aseptic environment, and then soak them in the growth-promoting agent for 60min. And after cutting off the roots, soak the remaining roots in the growth promoter for 5 minutes, immediately replant and water and apply organic fertilizers for daily weeding.

[0044] The preparation method of the growth-promoting agent is as follows: take the tree root tissue, crush it, and carry out biological enzymatic hydrolysis and fermentation to obtain the active peptide freeze-dried powder, and then prepare a solution with a mass concentration of 20%, and add 0.2% ...

Embodiment 2

[0050] A method for rapidly growing seedlings by tissue culture of mulberry roots, comprising the following steps:

[0051] 1) Cutting the roots and hairs: Cut off the branches of the female mulberry tree (the aerospace hybrid tree species of the Institute of Botany, Chinese Academy of Sciences) at a distance of 35 cm above the ground, dig up the soil of the female plexus tree, and wash the roots. Cut the root hairs of φ1-5mm into 3-4cm length under aseptic environment, and then soak them in the growth-promoting agent for 50 minutes. And after cutting off the roots, soak the remaining roots in the growth promoter for 3 minutes, immediately replant and water and apply organic fertilizers for daily weeding.

[0052] The preparation method of the growth-promoting agent is as follows: take the tree root tissue, crush it, and carry out biological enzymatic hydrolysis and fermentation to obtain the active peptide freeze-dried powder, then prepare a solution with a mass concentration...

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Abstract

The invention relates to the field of agriculture, and discloses a root hair tissue culture rapid seedling-raising method for broussonetia papyrifera. The method comprises the following steps: 1) cutting root hair: excavating the female parent broussonetia papyrifera, washing the root hair, cutting the root hair with a diameter of 1-5 mm to obtain root hair with a length of 3-4 cm, and soaking theobtained root hair in a growth promoting agent; 2) preparing a soilless nutrient substrate; 3) performing tissue culture; 4) exercising seedlings; and 5) performing field transplantation: 28-32 daysafter seedling exercising is performed, transplanting the root hair to a field. The method provided by the invention adopts the root hair of the broussonetia papyrifera for the tissue culture, the seedling raising period is short (seedlings only needs about 45 days to grow to 30 cm or more, and the seedlings can be transplanted to the field), the costs are low, the survival rate is high, and a variety in the later period is not easy to degrade.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to a method for rapidly growing seedlings through tissue culture of roots and hairs of mulberry trees. Background technique [0002] The mulberry tree is an ordinary tree species that has lived in the corners of the countryside for thousands of years in China. However, at present, the mulberry tree has become a high-protein forest tree that "stores grain in the forest and stores grain in technology" through modern biotechnology. Safety has far-reaching significance, and it can make the best use of everything to benefit mankind and society. [0003] The mulberry tree has strong adaptability, fast growth, high yield, strong vitality, well-developed root system, simple and extensive management, planting a tree to form a forest, afforestation in difficult areas (saline-alkali land, desert and rocky desert, desert area), and developing under-forest economy , mine vegetation restoration, and a...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G2/10
CPCA01G2/10A01G31/00Y02P60/21
Inventor 张建人张道平樊永俊
Owner 杭州早稻田生物技术有限公司
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