Method for efficiently inducing seedling propagation of paris polyphylla

Abstract

The invention discloses a method for efficiently inducing seedling propagation of paris polyphylla. According to the method, a paris polyphylla seed of which the radicle is already differentiated is adopted as explants, callus induction and propagation culture are carried out, after a high propagation rate is met, differential culture, tuber expansion, rooting culture and transplanting are furthercarried out, and then a great amount of paris polyphylla seedlings can be propagated, and the paris polyphylla medicinal material growth period can be shortened. By adopting the method, the problemsthat in the prior art paris polyphylla seedlings are low in propagation rate and slow in growth and requirements of the market on paris polyphylla seedlings cannot be met, can be solved. By adopting the method disclosed by the invention, the propagation rate can be up to 60000 times or greater within two years, and the propagation rate is much faster than that of common seed propagation. The method is not only high in propagation rate, but also capable of shortening the field growth period of the paris polyphylla medicinal material, in addition high in fixed planting survival rate of tissue culture tubers, and applicable to on-scale production of paris polyphylla.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for efficiently inducing the propagation of Huachonglou seedlings. Background technique [0002] Huachonglou, also known as Aescin, is a precious Chinese herbal medicine. Huachonglou seeds have the characteristics of low natural reproduction rate, incomplete embryo development, hard endosperm and post-ripening hypocotyl, so the seeds usually need two winters to germinate after sowing. Seedlings grow for a long time. However, conventional rhizome cut-section cultivation, because the soil temperature and humidity cannot be controlled, the incision healing is not ideal, and it is easy to infect the fungus and cause the whole rhizome to fester, and the loss of germplasm material is high; At the same time, there is less germination, which leads to a reduction in the overall reproductive coefficient and poor quality of seedlings produced by propagation. ...

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN201310529238.9 discloses a rapid propagation method of Huachonglou plants. This patent uses the rhizomes of Huachonglou as explants, which has the following disadvantages: because the rhizomes grow in the soil, the pollution of the primary culture is very serious In addition, since the rhizome itself is a medicinal material, and generally only one or two buds at the front can induce callus, this method will waste a lot of raw materials