Method for efficiently inducing seedling propagation of paris polyphylla
A technology of Huazhong and seedlings, which is applied in the field of plant breeding, can solve the problems of seriousness, waste of raw materials, pollution of primary culture, etc., and achieve the effects of strong anti-adversity ability, convenient management and high reproduction coefficient.
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Embodiment 1
[0047] A method for efficiently inducing the propagation of Huachonglou seedlings, comprising the acquisition of sterile explants: cultivating through the following steps:
[0048] 1) Disinfection treatment: select the Huachonglou seeds that have differentiated radicles, rinse them with running water, soak them in washing powder, brush them lightly with a brush, then rinse them with tap water, then cut off the radicles, and leave the rest As explants, the explants were soaked in 75% alcohol for 30 s on the ultra-clean workbench, then sterilized with 0.1% mercuric chloride for 10 min, and finally rinsed with sterile water 4 times;
[0049] 2) Induction culture: inoculate the sterilized explants on the induction medium, and culture them for 55 days at a temperature of 20 °C in the dark to obtain callus tissue;
[0050] The induction medium is MS medium with pH=5.8, and the components and their concentrations in the induction medium are as follows:
[0051] 6-BA 2.5mg·L -1
[...
Embodiment 2
[0078] A method for efficiently inducing the propagation of Huachonglou seedlings, comprising the acquisition of sterile explants: cultivating through the following steps:
[0079] 1) Disinfection treatment: select the Huachonglou seeds that have differentiated radicles, rinse them with running water, soak them in washing powder, brush them lightly with a brush, then rinse them with tap water, then cut off the radicles, and leave the rest As explants, the explants were soaked in 70% alcohol for 35 s on the ultra-clean workbench, then sterilized with 0.15% mercuric chloride for 8 min, and finally rinsed with sterile water for 3 times;
[0080] 2) Induction culture: the sterilized explants were inoculated on the induction medium, and cultured at a temperature of 18 °C in the dark for 60 days to obtain callus;
[0081] The induction medium is MS medium with pH=5.6, and the components and their concentrations in the induction medium are as follows:
[0082] 6-BA 2.0 mg·L -1
[...
Embodiment 3
[0109] A method for efficiently inducing the propagation of Huachonglou seedlings, comprising the acquisition of sterile explants: cultivating through the following steps:
[0110] 1) Disinfection treatment: select the Huachonglou seeds that have differentiated radicles, rinse them with running water, soak them in washing powder, brush them lightly with a brush, then rinse them with tap water, then cut off the radicles, and leave the rest As explants, the explants were soaked in 75% alcohol for 25s on the ultra-clean workbench, then sterilized with 0.1% mercuric chloride for 10 min, and finally rinsed with sterile water for 4 times;
[0111] 2) Induction culture: inoculate the sterilized explants on the induction medium, and culture them for 50 days at a temperature of 22 °C in the dark to obtain callus tissue;
[0112] The induction medium is MS medium with pH=6.0, and the components and their concentrations in the induction medium are as follows:
[0113] 6-BA 3.0 mg L -1 ...
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