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Biotechnology and application for repairing soil arsenic pollution

A kind of biotechnology and soil restoration technology, applied in the field of ecological environment protection, can solve the problems of super accumulation performance degradation, loss of repair function, poor repair effect, etc., and achieve simple and easy technology, low cost, and conducive to ecological sustainable development Effect

Inactive Publication Date: 2018-11-20
南京怡可帮生态环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these plants are shade plants, which grow in wild wilderness for a long time and are used for arsenic cultivation in large-scale farmland soil. After asexual reproduction, the performance of hyperaccumulation is greatly degraded, and the repair function is lost

Method used

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  • Biotechnology and application for repairing soil arsenic pollution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Add 70 grams of starch, 70 grams of bean cake powder, 7 grams of calcium carbonate powder, 30 grams of dipotassium hydrogen phosphate, 30 grams of potassium dihydrogen phosphate, 10 grams of ferrous sulfate, and 10 grams of magnesium sulfate in a 10-liter bioreactor , 7000 milliliters of water, stirring, after all mixing, start the heating steam pipe, heat the mixed solution, keep it at 126°C under 0.1Mpa for 40 minutes, after the sterilization is completed, cool to 30°C and inoculate 0.5% (mass / volume) malodorous fake form bacillus, start the agitator, the agitator speed is 50 revs / min, the ventilation rate is 0.5 liter of air / liter of liquid (volume / volume / minute), and the constant temperature mixed culture is 48 hours, and the fermentation is finished, and the tank is put. In the same way, inoculate 0.5% (mass / volume) of Alcaligenes faecalis and Flavobacterium respectively in the same liquid fermentation medium, start the agitator, the agitator rotating speed is 50...

Embodiment 2

[0024] 1. Add 300 grams of starch, 180 grams of soybean meal powder, 20 grams of calcium carbonate powder, 50 grams of dipotassium hydrogen phosphate, 50 grams of potassium dihydrogen phosphate, 30 grams of ferrous sulfate, 30 grams of magnesium sulfate into a 10-liter bioreactor , 7000 ml of water, stir, after all mixed, start the heating steam pipe, heat the mixed solution, keep it at 126 ° C for 40 minutes under 0.1 Mpa, the sterilization is over, cool to 30 ° C and inoculate 3% (mass / volume) of malodorous fake sheets Bacillus, start the stirrer, the stirrer speed is 90 rpm, the ventilation rate is 0.8 liters of air / liter of liquid (volume / volume / minute), and the constant temperature mixed culture is carried out for 38 hours. After the fermentation is completed, the tank is placed. In the same way, inoculate 3% (mass / volume) of Alcaligenes faecalis and Flavobacterium respectively in the same liquid fermentation medium, start the agitator, the agitator rotation speed is 90 re...

Embodiment 3

[0028] 1. Add 700 grams of starch, 280 grams of bean cake powder, 70 grams of calcium carbonate powder, 100 grams of dipotassium phosphate, 100 grams of potassium dihydrogen phosphate, 50 grams of ferrous sulfate, 50 grams of magnesium sulfate into a 10-liter bioreactor , 7000 ml of water, stir, after all mixed, start the heating steam pipe, heat the mixed solution, keep at 126 ° C for 40 minutes under 0.1 Mpa, the sterilization is over, cool to 30 ° C and inoculate 6% (mass / volume) of malodorous fake sheets Bacillus, start the stirrer, the stirrer speed is 120 rpm, the ventilation rate is 1 liter of air / liter of liquid (volume / volume / minute), and the constant temperature mixed culture is carried out for 24 hours. After the fermentation, the tank is placed. In the same way, inoculate 6% (mass / volume) of Alcaligenes faecalis and Flavobacterium respectively in the same liquid fermentation medium, start the agitator, the agitator rotating speed is 120 rev / min, and the ventilation ...

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Abstract

The invention discloses a biotechnology and application for repairing soil arsenic pollution, and belongs to the field of ecological environmental protection. The biotechnology comprises the steps that starch, bean cake, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferrous sulfate and the like are added into a bioreactor, and sufficient amount of water is thenadded into the bioreactor; stirring, mixing, heat sterilization, cooling, inoculation of pseudomonas putida and the like, fermentation, cooling and canning are carried out; the mixture is applied into soil; and natural cultivation is carried out for a period of time. Arsenic in the soil can be continued to convert into several arsenic-containing gases, the several arsenic-containing gases are volatilized from the soil into the air so as to remove arsenic in the soil, the soil arsenic pollution is eliminated, the soil environment is protected, the soil productivity is protected, the groundwater environment is protected, and the food safety is ensured.

Description

technical field [0001] The invention belongs to the field of ecological environment protection, and in particular relates to a biotechnology and application for remediating soil arsenic pollution. Background technique [0002] Arsenic is a very toxic environmental pollutant. The arsenic in natural soil mainly comes from the soil-forming parent material. During the formation of soil by rock weathering, the content of arsenic (relative to the soil-forming parent material) was significantly enriched. Human activities such as mining, smelting, sewage irrigation, fertilization, application of pesticides and wood preservatives may cause soil arsenic pollution. Excessive accumulation of arsenic in the soil will reduce crop yield and quality, and may cause water and air pollution, and can enter the human body through food chains and water bodies. Recent studies have shown that low levels of arsenic can seriously endanger human health. [0003] In 2016, the "Soil Pollution Preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 吴洪生
Owner 南京怡可帮生态环境科技有限公司