Unlock instant, AI-driven research and patent intelligence for your innovation.

Soil arsenic pollution restoration microbial preparation production method and application

A technology for a microbial preparation and a production method, which is applied to the production method and application field of a microbial preparation for soil arsenic pollution remediation, can solve the problems of hyperaccumulation performance degradation, poor remediation effect, loss of remediation function, etc. Sustainable, low-cost effects

Inactive Publication Date: 2018-10-12
南京怡可帮生态环境科技有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these plants are shade plants, which grow in wild wilderness for a long time and are used for arsenic cultivation in large-scale farmland soil. After asexual reproduction, the performance of hyperaccumulation is greatly degraded, and the repair function is lost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Soil arsenic pollution restoration microbial preparation production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Add 100 grams of starch, 60 grams of bean cake powder, 40 grams of dipotassium hydrogen phosphate, 30 grams of potassium dihydrogen phosphate, 10 grams of calcium carbonate powder, 10 grams of ferrous sulfate, and 10 grams of magnesium sulfate in a 10-liter bioreactor , 7000 milliliters of water, start the stirrer to stir the mixed solution, start the heating steam pipe, heat the mixed solution, keep it at 126°C under 0.1Mpa for 45 minutes, after the sterilization is finished, cool to 30°C to inoculate 0.5% (mass / volume) sulfur unit cells Bacteria, stirring continuously, the speed of the agitator is 50 rpm, the ventilation rate is 0.5 liters of air / liter of liquid / min, and the constant temperature is mixed for 24 hours. After the fermentation is completed, put it into the tank. In the same method, inoculate 0.5% (mass / volume) rhizobium, Alcaligenes faecalis, and Agrobacterium alberta respectively in the above-mentioned same liquid fermentation medium, start the agitat...

Embodiment 2

[0024] 1. Add 300 grams of starch, 100 grams of bean cake powder, 70 grams of dipotassium hydrogen phosphate, 80 grams of potassium dihydrogen phosphate, 50 grams of calcium carbonate powder, 30 grams of ferrous sulfate and 30 grams of magnesium sulfate in a 10-liter bioreactor. 7000 milliliters of water, start the stirrer to stir the mixed solution, start the heating steam pipe, heat the mixed solution, keep it at 126°C under 0.1Mpa for 45 minutes, after the sterilization is completed, cool to 30°C and inoculate with 3% (mass / volume) Thiomonas , continuous stirring, the speed of the agitator is 80 rpm, the ventilation rate is 0.8 liters of air / liter of liquid / min, and the constant temperature is mixed for 32 hours. After the fermentation is over, put the tank. In the same method, inoculate 3% (mass / volume) of rhizobia, Alcaligenes faecalis, and Agrobacterium alberta respectively in the above-mentioned same liquid fermentation medium, start the agitator, the agitator rotating s...

Embodiment 3

[0028] 1. Add 800 grams of starch, 200 grams of bean cake powder, 150 grams of dipotassium hydrogen phosphate, 140 grams of potassium dihydrogen phosphate, 150 grams of calcium carbonate powder, 60 grams of ferrous sulfate, and 60 grams of magnesium sulfate in a 10-liter bioreactor , 7000 milliliters of water, start the agitator to stir the mixed solution, start the heating steam pipe, heat the mixed solution, keep it at 126°C under 0.1Mpa for 45 minutes, after the sterilization is finished, cool to 30°C to inoculate 7% (mass / volume) sulfur unit cells Bacteria, stirring continuously, the speed of the agitator is 120 rpm, the ventilation rate is 1 liter of air / liter of liquid / min, and the mixed culture is kept at a constant temperature for 48 hours. After the fermentation is completed, put it into the tank. In the same way, inoculate 7% (mass / volume) of rhizobia, Alcaligenes faecalis, and Agrobacterium alberta respectively in the above-mentioned same liquid fermentation medium, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a soil arsenic pollution restoration microbial preparation production method and application, which belongs to the field of the protection of ecological environment. The production method comprises the following steps: adding starch, soybean meal, calcium carbonate, potassium dihydrogen phosphate, dipotassium phosphate, and ferrous sulfate into a bioreactor, then adding sufficient water, stirring, uniformly mixing, heating, sterilizing, cooling, inoculating microorganisms such as thiomonas, fermenting, cooling, blending, canning, applying into the soil, and naturally culturing for 6 to 18 months, so that arsenic in the soil can be continuously converted to the volatile gas to be volatilized to the air from the soil, the arsenic in the soil can be removed, the arsenic pollution of the soil and underground water can be eliminated, the soil environment can be protected, the soil production and ecological function can be protected, the underground water environmentcan be protected, and the food safety can be ensured.

Description

technical field [0001] The invention belongs to the field of ecological environment protection, and in particular relates to a production method and application of a soil arsenic pollution restoration microbial preparation. Background technique [0002] Arsenic is a very toxic environmental pollutant. The arsenic in natural soil mainly comes from the soil-forming parent material. During the formation of soil by rock weathering, the content of arsenic (relative to the soil-forming parent material) was significantly enriched. Human activities such as mining, smelting, sewage irrigation, fertilization, application of pesticides and wood preservatives may cause soil arsenic pollution. Excessive accumulation of arsenic in the soil will reduce crop yield and quality, and may cause water and air pollution, and can enter the human body through food chains and water bodies. Recent studies have shown that low levels of arsenic can seriously endanger human health. [0003] In 2016,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/41C12R1/05
CPCB09C1/10C12N1/20
Inventor 吴洪生
Owner 南京怡可帮生态环境科技有限公司