Method for increasing content of fucoxanthin in heterotrophic cultured smooth nitzschia fermentation liquid by utilizing illumination

A technology of heterotrophic culture and fucoxanthin, which is applied in the field of post-processing of fermentation liquid, can solve the problems such as the influence of fucoxanthin accumulation in the heterotrophic culture characteristics of algae that has not been explored, and achieve the effect of increasing fucoxanthin content.

Active Publication Date: 2018-11-20
PEKING UNIV
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Problems solved by technology

Heui-Chun et al. studied the effect of different light qualities (blue light, green light, red light and white light) on the accumulation of fucoxanthin in the marine diatom Chaetoceroscalcitrans, and found that blue light stimulated the accumulation of fucoxanthin, while red light stimulated the accumulation of fucoxanthin. Light inhibits the accumulation of fucoxanthin, but the algae used in this study is Chaetoceros, and it was cultivated by continuous irradiation with monochromatic light, and the heterotrophic culture characteristics of the algae and the effect on the accumulation of fucoxanthin in the cells after fermentation were not explored ( Bulletin of the Korean Society of Fisheries Technology, 2014, 50:447-454)
The applicant found that the effect of light quality on the accumulation of fucoxanthin in Nitzschia smoothis has not been reported so far

Method used

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  • Method for increasing content of fucoxanthin in heterotrophic cultured smooth nitzschia fermentation liquid by utilizing illumination
  • Method for increasing content of fucoxanthin in heterotrophic cultured smooth nitzschia fermentation liquid by utilizing illumination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Light-induced increase of fucoxanthin content in Nitzbug cells after fermentation

[0042] Nitzschia laevis UTEX 2047 (purchased from the Microalgae Collection of the University of Texas at Austin, Culture Collection of Algae at The University of Texas Austin, UTEX for short) was selected as the production strain.

[0043] The process steps are as follows:

[0044] A, adopting the method for heterotrophic cultivation to prepare Nitzschia smoothis fermented liquid, the substratum of heterotrophic cultivation comprises following raw material components:

[0045] NaCl 10g / L-32g / L; NaCl 2 SiO 3 9H 2 O 30mg / L-700mg / L; MgSO 4 ·7H 2 O 1.09g / L-2.18g / L; CaCl 2 2H 2 O 0.1g / L-0.27g / L; KH 2 PO 4 0.031g / L-0.062g / L; K 2 HPO 4 0.00375g / L-0.0075g / L; FeCl 3 ·6H 2 O 0.291mg / L-0.582mg / L; MnCl 2 4H 2 O 0.025mg / L-0.246mg / L; ZnCl 2 0.031mg / L-0.311mg / L; CoCl 2 ·6H 2 O 0.0114mg / L-0.0228mg / L; Na 2 MoO 4 2H 2 O 0.012mg / L-0.024mg / L; H 3 BO 3 3.06g / L-30.56g / L; (NH 4 ) 6 M...

Embodiment 2

[0052] The difference between this example and Example 1 is that the alga species in this example is Nitzschialaevis CCMP559 (purchased from the National Center for Marine Algae and Microbiota, NCMA for short).

Embodiment 3

[0054] The difference between this example and Example 1 is that the algal species in this example is Nitzschialaevis CCMP 1092 (purchased from the National Center for Marine Algae and Microbiota, NCMA for short).

[0055] The effect analysis of embodiment 1-3:

[0056] Under the culture condition of mixed light (blue light: white light = 1:1), the content of fucoxanthin in Nitzschia smoothis was up to 1.19% (accounting for cell dry weight), which was 58.7% higher than that of heterotrophic fermentation without light.

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Abstract

The invention belongs to post-treatment of fermentation liquid, and particularly relates to a method for increasing the content of fucoxanthin in heterotrophic cultured smooth nitzschia fermentation liquid by utilizing illumination. The method comprises process processing steps of preparing smooth nitzschia fermentation liquid by adopting a heterotrophic culture method; transferring the prepared smooth nitzschia fermentation liquid into a photobioreactor under the illumination of single-colored light or hybrid light, performing ventilation for induced culture under the illumination condition and the like. The method solves the technical problem that the content of the fucoxanthin in the smooth nitzschia fermentation liquid prepared by heterotrophic culture is low in the prior art. The method has the advantages that the content of the fucoxanthin in prepared dry smooth nitzschia powder is high, continuous industrial production can be stably realized, general pollutants in the ocean suchas heavy metals and polychlorinated biphenyl can be controlled from the source of a culture medium, and the fucoxanthin from relatively large-sized alga is safer.

Description

technical field [0001] The invention belongs to post-treatment of fermented liquid, in particular to a method for increasing the content of fucoxanthin in the fermented liquid of Nitzschia smoothis by using light. Background technique [0002] Fucoxanthin is a natural carotenoid, also known as fucoxanthin and fucoxanthin, which participates in the reaction of photosystem II in photosynthesis. Fucoxanthin has a unique allene structure and is an active molecule with strong antioxidant properties. It has been confirmed to have multiple functional activities in cells, animals and humans, including anti-obesity, anti-diabetes, anti-inflammation, anti- Cancer, anti-angiogenic and anti-malarial activities. Based on the above functions, fucoxanthin is a natural product with broad prospects for health food and drug development, and has huge market value. [0003] Currently on the market, fucoxanthin is mainly extracted from large seaweeds such as Undaria pinnatifida and Laminaria j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12R1/89
CPCC12P17/02
Inventor 刘宾陈峰卢雪
Owner PEKING UNIV
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