Anti-Sweet Potato Rhizorrhizae Compound Essential Oil Preparation, Its Preparation Method and Its Application in Controlling Sweet Potato Black Spot
A technology of sweet potato longbeak fungus and compound essential oil, which is applied in the directions of botanical equipment and methods, applications, fungicides, etc., can solve problems such as threats to human and animal health, pesticide residues, environmental pollution, etc., and achieves simple control methods and low production costs. , good control effect
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Embodiment 1
[0019] Embodiment 1: the preparation of anti-sweet potato rostral fungus compound essential oil preparation
[0020] Take dried clove root, turmeric and dried eucalyptus leaves, and weigh corresponding materials according to the mass ratio of 2:1:3. put into a figure 1 In the shown essential oil extraction device, the water vapor is distilled, and the distillate is cooled and collected for later use. Add analytically pure ethanol into the ether solution, and the final concentration of ethanol is 2% (volume ratio). Use the prepared diethyl ether solution to extract the distillate, place it in a vacuum distillation device, and heat it in a water bath at 30°C under vacuum to recover diethyl ether. The device was changed into a rectification device, and refined under reduced pressure at 40°C to obtain a compound essential oil preparation resistant to S. elongatus.
Embodiment 2
[0021] Example 2: Composition identification of anti-sweet potato rostral fungus compound essential oil preparation
[0022] Acetone was used to dissolve the compound essential oil preparation against C. spp., and GC-MS (gas phase-mass spectrometry) instrument was used to analyze the components in the compound essential oil preparation against C. spp., the instrument parameters were as follows:
[0023] Gas phase conditions: injection port at 100°C, splitless injection, carrier gas of high purity helium, flow rate of 1.0mL / min, septum purge of 5mL / min. Using the temperature program mode, the initial temperature of the column oven was 100°C, kept for 5 minutes, raised to 250°C at a rate of 10°C / min, kept for 5 minutes, then raised to 280°C at a rate of 2°C / min, and kept for 15 minutes. Mass spectrometry conditions: the transfer line temperature is 280°C, the ion source is an EI ion source, the temperature is 280°C, and the scanning range is 35-650m / z.
[0024] GC-MS analysis r...
Embodiment 3
[0028] Example 3: Effects of Anti-Sweet Potato Racinorrhizae Compound Essential Oil Preparation on Mycelia Growth and Sporulation of S.
[0029] Prepare PDA medium, the steps are as follows: peeled potatoes 200g, glucose 25g, agar 20g, distilled water 1000mL, pH6.9, after dissolving, sterilize at 115°C for 30min, when cooled to 40°C, add anti-sweet potato beak in proportion For the shell fungus compound essential oil preparation, mix well to obtain the medium with the final concentration of essential oil of 0.10, 0.20 and 0.50 mg / mL, and pour it into a disposable petri dish for use. Use a hole puncher (1cm) to punch holes at the edge of the colony of S. longinosaum for about 11 days to obtain the fungus cake. Put the hyphae down into the above-mentioned medium of different concentrations of essential oil preparations, and place it in an incubator at 25°C. In the in vitro culture experiment, 3 replicates were set up for each treatment. The PDA plate without adding the anti-swe...
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