Bifidobacterium bifidum feed additive

A Bifidobacterium bifidum and feed additive technology, which is applied in the field of Bifidobacterium bifidum feed additives and Bifidobacterium feed additives, can solve the problems of irritation, no direct use of nutritional value for breeding animals, and strict anaerobic conditions.

Inactive Publication Date: 2018-11-23
JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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AI-Extracted Technical Summary

Problems solved by technology

Compared with lactic acid bacteria, bifidobacteria have the following disadvantages: (1) strict and complex requirements for nutrients, the medium must contain auxin or yeast extract to grow; (2) anaerobic conditions are more stringent; (3) at pH Short survival time in low environment
[0005] In order to keep the pH value in the stomach of the animals relatively constant, so that the fed food can continue to achieve the effect of acidification after entering the small intestine, acidulants such as citric acid, lac...
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Abstract

The invention discloses a bifidobacterium bifidum feed additive. The bifidobacterium bifidum feed additive is characterized by being prepared by uniformly mixing bifidobacterium bifidum, fructus hippophae rhamnoides freeze-dried powder, Fructus Rosae Roxburghii freeze-dried powder, Calyx seu Fructus Physalis freeze-dried powder, blackcurrant freeze-dried powder and cholla root and stem freeze-dried powder, then adding the balance of carbonized eggshell powder, then uniformly mixing, and carrying out composite packaging. Per 1000g of the feed additive is prepared from 1000-12000 billions of bifidobacterium bifidum, 5-50g of sea-buckthorn freeze-dried powder, 5-50g of roxburgh rose freeze-dried powder, 3-30g of Calyx seu Fructus Physalis freeze-dried powder, 3-30g of blackcurrant freeze-dried powder, 50-150g of cholla root and stem freeze-dried powder and the balance of carbonized eggshell powder. According to the bifidobacterium bifidum feed additive, the viable count is clear; application and quantification selection of the user are facilitated; the sea-buckthorn freeze-dried powder, the roxburgh rose freeze-dried powder, the Calyx seu Fructus Physalis freeze-dried powder, the blackcurrant freeze-dried powder, the cholla root and stem freeze-dried powder and the carbonized eggshell powder are used as base materials of the additive; the stability, the trophism and the adsorptivity of the bacteria in the additive can be improved; meanwhile, the nutrition value of the additive is increased; the bifidobacterium bifidum feed additive is capable of accelerating the metabolism of animals, improving disease resistance of organisms, accelerating the growth of the animals and finally increasing the economic benefit of the breeding industry.

Application Domain

Digestive systemCarbon active ingredients +5

Technology Topic

Bifidobacterium bifidumBifidobacterium +13

Examples

  • Experimental program(12)

Example Embodiment

[0050] Example 1
[0051] Preparation of freeze-dried seabuckthorn powder, freeze-dried prickly pear powder, freeze-dried physalis powder, freeze-dried blackcurrant powder, and freeze-dried cactus powder:
[0052] Take fresh and ripe sea buckthorn, prickly pear, physalis, blackcurrant, cactus, remove the stalks, rotten fruits and other impurities, use a 40 ℃ aqueous solution containing 0.1% by weight of citric acid, rinse for 15 minutes under constant stirring, remove and put in Rinse 3 times in pure water, remove the dry water; put seabuckthorn, prickly pear, physalis, blackcurrant, and cactus into the freezer, and quickly cool down to below -45℃; respectively, freeze seabuckthorn and prickly pear , Physalis, blackcurrant, and cactus are moved into the sublimation dryer, the sublimation temperature is controlled at -22-32℃, the vacuum degree is 120-150Pa, and the material is dried to moisture <6% (w). It takes about 18 hours to take it out. Then put it into a freeze-dried pulverizer, control the temperature of the material to not exceed 0℃, and pulverize to a fineness of >100 mesh, and then obtain sea-buckthorn freeze-dried powder, prickly pear freeze-dried powder, physalis freeze-dried powder, black currant freeze-dried powder, The lyophilized cactus powder is sealed in a plastic bag and stored in a refrigerator below 0°C for later use.

Example Embodiment

[0053] Example 2
[0054] Preparation of carbonized eggshell powder:
[0055] First, clean the eggshell, dry the water, put it in an electric oven, dry it with hot air at 200℃ for 2 hours, take it out and let it cool, crush it into 20 mesh coarse powder, and place it in the egg according to the weight ratio of 1:0.5. Spray the prepared salt solution with a weight ratio of 25% on the coarse shell powder (ie egg shell powder: 25% salt solution = 1:0.5 ratio), and mix while spraying. After mixing, let it stand for 6 hours, and then mix it evenly. Put the soaked eggshell powder raw materials into the rotary carbonization pot, rotate and carbonize at 420℃ for 80 minutes, observe that the raw materials are completely blackened (the carbonization process is equipped with an automatic rotating scraper in the carbonization pot To prevent the raw materials from sticking to the pot and agglomerate during the carbonization process); quickly move the carbonized raw materials to the activation furnace, and pass CO 2 Gas, drive out the air in it, anaerobic activation at 760°C for 80 minutes (a controllable stirring and turning blade is set in the activation furnace, so that the activated material is turned once every 10 minutes to ensure the complete and uniform activation of the material); After activation, take out the activated material and let it cool to normal temperature. Rinse the activated material with clean water in the cleaning tank until the sodium chloride is basically no longer detected. After cleaning the sodium chloride, use 12% hydrochloric acid by mass Submerge the activated material for 2.5 hours. After the soaking is complete, wash the activated material with 95℃ pure water until the pellets are no longer white, then rinse with pure water at room temperature until the pH value is 7.0, filter, and rinse the activated material; rinse it clean The activated material is dried at 115°C until the water content is less than 6%, passed through a 60-mesh sieve, and the sieved coarse particles are then crushed to make all of them pass through the 60-mesh sieve to obtain a charred eggshell powder product.

Example Embodiment

[0056] Example 3
[0057] Preparation of carbonized eggshell powder:
[0058] First, clean the eggshell, dry the water, place it in a far-infrared dryer, dry at 300℃ for 1 hour, take it out and let it cool, crush it into 30 mesh coarse powder, and place it in the eggshell at a weight ratio of 1:0.5. Spray the prepared salt solution with a weight ratio of 25% on the coarse powder (ie eggshell powder: 25% salt solution = 1:0.5 ratio), and mix while spraying. After mixing, let it stand for 7 hours, and then mix it evenly. Leave it for another 7 hours; put the soaked eggshell powder raw materials into the rotary carbonization pot, rotate and carbonize at 450°C for 70 minutes, and observe that the raw materials are completely blackened (the carbonization process is equipped with an automatic rotating scraper in the carbonization pot , To prevent the raw materials from sticking to the pan and agglomeration during the carbonization process); quickly move the carbonized raw materials to the activation furnace, and pass CO 2 Gas, drive out the air, anaerobic activation at 800°C for 55 minutes (a controllable stirring and turning blade is set in the activation furnace, so that the activated material is turned once every 10 minutes to ensure the complete and uniform activation of the material); After activation, take out the activated material and let it cool to normal temperature. Rinse the activated material with clean water in the cleaning tank until the sodium chloride is basically no longer detected. After cleaning the sodium chloride, use 12% hydrochloric acid by mass Immerse the activated material for 3 hours. After the soaking is complete, wash the activated material with 95℃ pure water until the pellets are no longer white, then rinse with pure water at room temperature until the pH value is 7.0, filter, and rinse the activated material; The activated material is dried at 120 DEG C to a moisture content of less than 6%, passed through a 60-mesh sieve, and the sieved coarse particles are then crushed to make all of them pass through the 60-mesh sieve to obtain a charred eggshell powder product.

PUM

PropertyMeasurementUnit
Water content<= 0.06

Description & Claims & Application Information

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