Extracting-purifying method for coenzyme Q10 and prepared coenzyme Q10

A purification method and coenzyme technology, applied in quinone separation/purification, organic chemistry and other directions, can solve problems such as difficulty, improper process design, emulsification phenomenon, etc., and achieve the effects of simple steps, small loss and low cost

Active Publication Date: 2018-11-23
ZHEJIANG NHU CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, the purification process reported so far has the following problems: unclear impurity distribution, poor effect of silica gel chromatography pretreatment process, emulsification phenomenon caused by improper process design, and complicated treatment of crystallization mother liquor, etc.

Method used

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  • Extracting-purifying method for coenzyme Q10 and prepared coenzyme Q10

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] i) extract

[0054] Take 780g of coenzyme Q10 bacteria (the content of coenzyme Q10 is 5.0%, the water content is 2.1%), after simple grinding, add n-hexane:methanol:water with a volume ratio of 9:3:1 (v / v / v) Mixed solvent 8L. The temperature is controlled at 25°C, and after mechanical stirring, it is left to stand for stratification. The upper layer is the n-hexane phase containing the product, and the lower layer is the methanol / water solution containing the bacteria residue. After the oil-water two phases were separated, the supernatant was evaporated in a rotary evaporator at 40°C to obtain 100.14 g of coenzyme Q10 extract. The measured content of coenzyme Q10 was 38.9%, and the yield was 99.8%. The methanol / water solution containing bacteria residue in the lower layer undergoes solid-liquid separation after sedimentation and filtration, and the solvent can be recycled for reuse. ii) Precipitation

[0055] Melt the coenzyme Q10 extract obtained in the extraction ...

Embodiment 2

[0061] The steps i) and step ii) of coenzyme Q10 extraction and purification were carried out in the same manner as in Example 1, wherein the coenzyme Q10 extract obtained after step i) was 98.54 g, the content of coenzyme Q10 was 39.5%, and the yield was 99.8%, the impurity-removed processed product obtained after step ii) precipitation is 56.48g, the content of coenzyme Q10 is 68.7%, and the yield is 99.7%. The treated product after removal of impurities is subjected to the adsorption decolorization operation of the following step iii).

[0062] First, dissolve 56.48g of the impurity-removed treatment in 800mL of n-hexane, then spread 2 layers of filter paper at the bottom of the φ7cm Buchner funnel, then take 30.86g of powdered activated clay, add n-hexane and spread it on the filter paper, and finally Spread 1 layer of filter paper on the stuffing. Suction filter the coenzyme Q10 solution dissolved in n-hexane with a Buchner funnel until the dripping liquid has no color a...

Embodiment 3

[0065]Coenzyme Q10 was extracted in the same manner as step i) of Example 1, except that cyclohexane:ethyl acetate:water was used in a mixed solvent with a volume ratio of 8:5:1 (v / v / v). The coenzyme Q10 extract obtained after separating the oil-water two phases and rotary evaporation was 100.01 g, the measured coenzyme Q10 content was 38.8%, and the yield was 99.5%.

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Abstract

The invention relates to an extracting-purifying method for coenzyme Q10 and prepared coenzyme Q10. The extracting-purifying method is used for extracting coenzyme Q10 from coenzyme Q10 thallus, and purifying the extracted coenzyme Q10. The method comprises the following steps of extraction, precipitation, decolorization and chromatography. Impurities such as bacterial pigments, nonpolar lipids, neutral lipids, polar lipids and quinones homologs are removed specifically. The method has the advantages of simple operation steps, and small product loss. The product coenzyme Q10 can be 99.8 percent or more in purity, and can be 98.5 percent or more in total yield.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-efficiency extraction and purification method for coenzyme Q10 and coenzyme Q10 prepared therefrom. Background technique [0002] As an antioxidant, coenzyme Q10 can remove free radicals in the human body, and has health effects of anti-aging and improving cardiovascular immunity, so the research on its preparation process has high industrial value. [0003] At present, coenzyme Q10 products are obtained through fermentation and extraction. The main manufacturers include Xiamen Jindawei, Japan KANEKA, Shenzhou Biology, Livzon Group, Zhejiang Medicine and NHU. Coenzyme Q10 is a fat-soluble substance with poor water solubility, so most of the current extraction processes are based on non-polar solvent extraction, such as petroleum ether and n-hexane. Of course, in order to extract completely, mixed solvents can also be used. For example, Patent Document 1 discloses A mixed solv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/10C07C50/28
CPCC07C46/10C07C50/28C07C2601/16
Inventor 闵一陈召峰张锦阳涂仕春胡伟江
Owner ZHEJIANG NHU CO LTD
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