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Culture medium of umbilical cord mesenchymal stem cells

A technology of stem cells and culture medium, applied in the field of stem cell culture, can solve the problems of introducing pathogens and xenogeneic antigens, and achieve the effect of promoting cell growth, high safety, and improving antioxidant capacity.

Inactive Publication Date: 2018-11-23
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a culture medium for umbilical cord mesenchymal stem cells, which solves the problem that the animal-derived serum in the prior art will introduce pathogens and heterogeneous antigens, and enables the cells to maintain a good state and proliferation speed, and to cultivate Good antioxidant effect

Method used

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  • Culture medium of umbilical cord mesenchymal stem cells
  • Culture medium of umbilical cord mesenchymal stem cells
  • Culture medium of umbilical cord mesenchymal stem cells

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preparation example Construction

[0016] Umbilical cord mesenchymal stem cells were prepared as follows:

[0017] (1) Wash the umbilical cord: transfer the umbilical cord to a 10cm sterile petri dish with sterile toothed forceps, add specimen washing solution to rinse, and repeat 2 to 3 times to remove blood stains as much as possible.

[0018] (2) Sterile tissue scissors Cut the umbilical cord into sections of about 2-3 cm, add 10-20 ml of specimen washing solution to wash the blood clot, and repeat the washing until the blood stains are basically removed, and the washing solution is clear. Eliminate blood vessels: remove two arteries and one vein of the umbilical cord according to the helical trend of blood vessels.

[0019] (3) Wash the blood stains from the umbilical cord tissue from which blood vessels have been removed with an appropriate amount of specimen washing solution, and repeat the washing until the washing solution is clear.

[0020] (4) Put the washed umbilical cord into a 10 cm sterile petri ...

Embodiment 1

[0024] A culture medium for umbilical cord mesenchymal stem cells, comprising DMEM / F12 medium, supplementary components and ivy extract added in the DMEM / F12 medium, the supplementary components include components of the following final concentration: human serum white Protein 1.25g / L, lysozyme 10mg / mL, transferrin 7.5mg / L, fibronectin 8mg / L, laminin 4mg / L, transferrin 10mg / L, Fe(NO 3 ) 3 9H 2 O 50g / L, FeSO 4 ·7H 2 O 417g / L, estradiol 1.3μg / L, testosterone 2μg / L, progesterone 1.3μg / L, dexamethasone 78.49μg / L, insulin 7mg / L, glucagon-like peptide 12μg / L, riboflavin Vegetarian 376.36mg / L, coenzyme A161.91mg / L, butanediamine 5.30mg / L, taurine 1.25mg / L, aminoethanol 1.22mg / L, pyruvate 22.42mg / L, sodium selenate 5.67μg / L , vascular endothelial growth factor 6 μg / L, basic fibroblast growth factor 10 μg / L, arginine 20 mg / L, leukemia inhibitory factor 1 μg / L, stem cell factor 8 μg / L, β-cytokinin 10 μg / mL, parathyroid Hormone 35.74ng / L, platelet-derived growth factor AB2.2μg / L, tu...

Embodiment 2

[0027] A culture medium for umbilical cord mesenchymal stem cells, comprising DMEM / F12 medium, supplementary components and ivy extract added in the DMEM / F12 medium, the supplementary components include components of the following final concentration: human serum white Protein 5g / L, lysozyme 10mg / mL, transferrin 5mg / L, fibronectin 2mg / L, laminin 4mg / L, transferrin 5mg / L, Fe(NO 3 ) 3 9H 2 O 50g / L, FeSO 4 ·7H 2 O 417g / L, estradiol 3μg / L, testosterone 5μg / L, progesterone 1μg / L, dexamethasone 39.25μg / L, insulin 10mg / L, glucagon-like peptide 12μg / L, riboflavin 376.36 mg / L, coenzyme A161.91mg / L, butanediamine 5.30mg / L, taurine 1.25mg / L, aminoethanol 0.61mg / L, pyruvate 22.42mg / L, sodium selenate 3.78μg / L, blood vessels Endothelial growth factor 2 μg / L, basic fibroblast growth factor 4 μg / L, arginine 10 mg / L, leukemia inhibitory factor 1 μg / L, stem cell factor 8 μg / L, beta cell factor 5 μg / mL, parathyroid hormone 71.48 ng / L, platelet-derived growth factor AB 0.5 μg / L, tumor necro...

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Abstract

The invention provides a culture medium of umbilical cord mesenchymal stem cells. The culture medium comprises a DMEM (Dulbecco's Modified Eagle Medium) / F12 culture medium, an adding component added into the DMEM / F12 culture medium and ivy extract, wherein the adding component is prepared from the following components: human serum albumin, transferrin, a vascular endothelial growth factor, a basicfibroblast growth factor, arginine, a leukemia inhibitory factor, a stem cell factor, beta cytokine, parathyroid hormone, a platelet-derived growth factor, a tumor necrosis factor alpha, interleukin,erythropoietin, thrombopoietin, alpha-D-glucose, vitamin C, resveratrol, NaHCO3 and ganglioside; the ivy extract is liquid and is extracted from leaves of ivy through a conventional method; the finalconcentration is 20 to 50mg / L after the ivy extract is added. By adopting the culture medium, the problems in the prior art are solved, and the cells can keep good state and proliferation speed.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a medium for umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects. Human umbilical cord mesenchymal stem cells can be successfully expanded by using the inactivated umbilical cord serum culture system, and the cultured cells have the basic characteristics of mesenchymal stem cells, which provides a theoretical basis for the establishment of mesenchymal stem cell banks and clinical applications. Mesenchymal stem cells have a strong immune regulation effect, can promote hematopoietic recovery, and can repair damaged or diseased tissues and organs. At present, there are many mesenchymal stem cell culture medium products on ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/12C12N2500/24C12N2500/30C12N2500/32C12N2500/34C12N2500/38C12N2500/44C12N2500/76C12N2501/115C12N2501/125C12N2501/145C12N2501/165C12N2501/23C12N2501/25C12N2501/33C12N2501/335C12N2501/73C12N2501/998
Inventor 宫丽董振磊张炳强戚凤春章宏金铎于淼
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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