A kind of measuring method of phenol content in water

Abstract

The invention discloses a method for measuring phenol content in water. The key points of the technical scheme include the following steps: Step 1: Take 10-50mL of water samples to be tested in a pressure vessel, add 5-20g of sodium chloride, and add 5-20mL of Organic solvent; step 2: put the above container into a muffle furnace to heat, then naturally cool to room temperature, collect the organic solvent in the upper layer, and use a rotary evaporator to concentrate; step 3: purify through a solid phase extraction column, collect the effluent and wash Deliquification; Step 4: Concentrate to near dryness with a nitrogen blower, add solvent to redissolve, and filter with a 0.02 to 0.45um organic filter; Step 5: Use a high-performance liquid chromatography to test phenol, and select a C8 or C18 liquid phase for the chromatographic column Chromatographic column; the mobile phase speed is set to 0.5-2mL / min, the injection volume is set to 20-100uL, and the column temperature is 20-50°C; a fluorescence detector is used for analysis. The method for measuring the phenol content in water of the present invention can detect the phenol content in the sample simply, quickly, and accurately qualitatively and quantitatively.

Description

【Technical field】 [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for measuring phenol content in water. 【Background technique】 [0002] People's requirements for food safety are getting higher and higher, and the limits of harmful substances in drinking water, surface water and agricultural irrigation water are becoming stricter and stricter. Phenol is widely used as a raw material for plastics, as an important raw material for producing resins, fungicides, preservatives and pharmaceuticals. However, phenol is a toxic substance, which has a strong corrosive effect on the skin and mucous membranes, can inhibit the central nervous system or damage liver and kidney functions; inhalation of high-concentration steam can cause headache, dizziness, fatigue, blurred vision, pulmonary edema, etc.; Taking it can cause digestive tract burns, burning pain, exhaled breath with phenol smell, vomit or stool with blood, there is...

AI Technical Summary

Problems solved by technology

However, the above method has the following defects: the pretreatment method needs to distill the water sample, then collect the distillate, and develop the color of the distillate, or use an organic solvent after the color development to extract and concentrate the color-developed material for testing. The process is complex and cumbersome, the pH value of the water needs to be considered, and a wide variety of reagents need to be configured, and the process is difficult to control. When using colorimetric testing, the color of the reagents and the color of the water sample will seriously interfere with the test results

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