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Novel human antimicrobial peptide LL-37 derivative and application thereof

A human antimicrobial peptide, LL-37 technology, applied in the field of molecular biology, can solve the problems of inability to realize large-scale production, complex process, expensive cost, etc., to solve the problem of antibiotic abuse, good antibacterial effect, and stability improvement Effect

Active Publication Date: 2018-11-27
珠海中科先进技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problems of low yield, long time-consuming, complicated process and high cost in extracting natural antimicrobial peptides from animals, it is impossible to achieve large-scale production, which has become the biggest obstacle restricting the clinical application of antimicrobial peptides.

Method used

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  • Novel human antimicrobial peptide LL-37 derivative and application thereof
  • Novel human antimicrobial peptide LL-37 derivative and application thereof
  • Novel human antimicrobial peptide LL-37 derivative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 of the present invention is: a novel human antimicrobial peptide LL-37 derivative, the amino acid sequence of which is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys -Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Gln-Asn, molecular weight 3177.6D. As shown in Table 1, the novel human antimicrobial peptide LL-37 derivatives include the antibacterial activity structure of the antimicrobial peptide LL-37 Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe- Leu-Arg.

[0016] Peptide name

amino acid sequence

Antimicrobial peptide LL-37

LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES

Antimicrobial Peptide LL-37 Derivatives

LLDFKERKEKQFKRIVQRIKDFLRQN

[0017] The antimicrobial peptide LL-37 derivative modified by the scheme of the present invention is rich in glutamine and cysteine, which enhances the stability of the antimicrobial peptide. Adding asparagine and lysine can attract negative charges on the surface of bacteria and enhance an...

Embodiment 2

[0019] Example 2 of the present invention is: preparation of a novel human antimicrobial peptide LL-37 derivative sodium chloride injection: this embodiment is the preparation of antibacterial drugs for the novel human LL-37 antimicrobial peptide derivative of the present invention The amino acid sequence of the antimicrobial peptide derivative is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile -Lys-Asp-Phe-Leu-Arg-Gln-Asn with a molecular weight of 3177.6D. This antimicrobial peptide derivative is made into polypeptide bacteriocin sodium chloride injection, and every 100ml injection is made up of following components: main agent is the antimicrobial peptide 0.5g of SEQ ID NO:1 sequence, and its percentage is 0.5%, chlorination Sodium 0.9g, the balance is water for injection.

[0020] Weigh the water for injection of 60% of the total volume of the prescribed amount, then add the prescribed amount of SEQ ID NO: 1 sequence antimicrobial peptide deriv...

Embodiment 3

[0021] The third embodiment of the present invention is: the preparation of a novel human-derived antimicrobial peptide LL-37 derivative gel: this embodiment is the application of the novel human-derived LL-37 antimicrobial peptide derivative in the preparation of antibacterial drugs. The amino acid sequence of the antimicrobial peptide derivative is Leu-Leu-Asp-Phe-Lys-Glu-Arg-Lys-Glu-Lys-Gln-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp- Phe-Leu-Arg-Gln-Asn, the molecular weight is 3177.6D. The antimicrobial peptide derivative is made into a polypeptide gel, and every 1000g of the gel is composed of the following components: the antimicrobial peptide 10g of the main drug SEQ ID NO:1 sequence, the auxiliary material Carbomer-940 10g, lauryl azepine Ketone 50ml, glycerin 200ml, Span-85 50ml, triethanolamine 20ml, and the balance is water for injection.

[0022] Weigh 10 g of the excipient Carbomer-940 powder, add an appropriate amount of water for injection and mix well, then add 5...

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Abstract

The invention discloses a novel human antimicrobial peptide LL-37 derivative and an application thereof. The amino acid sequence of human antimicrobial peptide LL-37 derivative is shown as SEQ ID NO:1. The invention further comprises an application of the novel human antimicrobial peptide LL-37 derivative in preparing antimicrobial agents. The antimicrobial agents are antibacterial drugs or anti-fungal drugs. The bacteria or fungi are at least one of cryptococcus, histoplasma capsulatum, monilia albicans, staphylococcus aureus, streptococcus pneumoniae, escherichia coli and salmonella. Compared with the prior art, the human antimicrobial peptide LL-37 derivative with the sequence, provided by the invention, has the advantages of high stability, excellent antimicrobial effect and the like,and has a wide application prospect in the field of antimicrobial drug preparation.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a novel human antimicrobial peptide LL-37 derivative and its application. Background technique [0002] Antimicrobial peptides are small molecular polypeptides with broad-spectrum antibacterial activity induced by organisms, usually composed of 12-60 amino acids, which are an inherent part of the body's non-specific defense system, and widely exist in bacteria, fungi, and amphibians , insects, higher plants, mammals and even humans. In recent years, due to the abuse of antibiotics leading to bacterial resistance, allergic reactions and environmental pollution, people began to urgently look for new drugs to solve the negative effects of antibiotics. Antimicrobial peptides have the advantages of good thermal stability, broad antibacterial spectrum, no pollution, and no drug resistance. They have become a research and development hotspot at home and abroad, gradually replacing anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K38/17A61P31/04A61P31/10
CPCA61K38/00A61P31/04A61P31/10C07K14/4723Y02A50/30
Inventor 徐畅李军华谢苍桑张溪之张利鹏姜长安
Owner 珠海中科先进技术研究院有限公司
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