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Bacterial strain with drought stress resisting function and application of bacterial strain

A drought-resistant and functional technology, applied in the field of microorganisms, can solve the problems of Serinicoccus bacteria against drought stress and other problems

Pending Publication Date: 2018-11-27
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the resistance of Serinicoccus bacteria to drought stress shocks

Method used

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  • Bacterial strain with drought stress resisting function and application of bacterial strain
  • Bacterial strain with drought stress resisting function and application of bacterial strain
  • Bacterial strain with drought stress resisting function and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Obtaining of Serinecoccus altae W204

[0022] Soil samples were collected from the grassland among birch forests in the Altay region of Xinjiang, 1g of soil samples were weighed, added to 20mL of TGY medium for enrichment and culture at 30°C and 200rpm for 112h, 100μL of the enrichment culture was taken, and 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 Sequentially serially dilute, take 200μL respectively and spread on R 2 A agar medium plate was placed upside down in an incubator at 30°C for several days, and the drought-tolerant Serinecoccus Altayae W204 of the present invention was isolated.

[0023] The R 2 A agar medium: casein hydrolyzate 0.5g / L; yeast extract 0.5g / L; tryptone 0.5g / L; glucose 0.5g / L; soluble starch 0.5g / L; dipotassium hydrogen phosphate 0.3g / L ; Anhydrous magnesium sulfate 0.024g / L; Sodium pyruvate 0.3g / L; Sodium chloride 10g / L; Agar 15g / L. pH 7.0-7.4.

[0024] The culture temperature was 37°C.

Embodiment 2

[0025] Example 2 The Drought Tolerance Experiment (Sorbitol Tolerance Test) of Serinecoccus Altayae W204

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Abstract

The invention discloses serinicoccus altayensis W204 with the collection number of GDMCC No: 60235. The serinicoccus altayensis has drought stress resistance, can serve as an important gene source forresisting drought stress and is used for building a transgenic organism and culturing a drought stress tolerant transgenic plant.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a bacterial strain with a drought-tolerant function and an application thereof. Background technique [0002] Among many abiotic stresses, drought is the most important stress factor limiting plant growth, affecting almost every stage of plant growth and development. It is the key to solve this problem to find drought stress resistance genes from the existing biological resources in nature and transform them into plants for expression, so as to improve the tolerance of plants to drought. [0003] At present, it has been reported that most of the microorganisms with drought resistance are bacteria of the genus Deinococcus, such as Deinococcus radiodurans. D. radiodurans has formed its own powerful DNA repair system during the long evolution of drought resistance. However, there is no report on the resistance of Serinicoccus bacteria to drought stress shocks. Contents of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/82A01H5/00C12R1/01
CPCC12N15/8273C12N1/205C12R2001/01
Inventor 林敏张维郭倩楠陈明周正富陆伟战嵛华燕永亮
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI