Method of improving antibacterial performance of nanosilver
A nano-silver antibacterial and nano-silver technology, applied in the field of antibacterial materials, can solve complex problems and achieve the effects of easy acquisition, strong microbial toxicity, and improved antibacterial performance
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Embodiment 1
[0027] A method for improving the antibacterial properties of nano silver, comprising the following steps:
[0028] (1) Preparation of nano-silver: 1.5mL of trisodium citrate solution with a concentration of 24mmol / L and 4.5mL of sodium borohydride solution with a concentration of 24mmol / L were added to 53.5mL of ultrapure water. Stir vigorously, and at the same time add 0.5 mL of silver nitrate solution with a concentration of 24 mM while stirring, and the mixed solution turns yellow, indicating that nano-silver is generated. Then continue to stir at room temperature for 3 h, and the stirred nano-silver solution is purified through a 1KDa dialysis membrane to remove soluble impurities, such as trisodium citrate and silver ions, to obtain a nano-silver stock solution.
[0029] (2) Preparation of Phanerochaete chrysosporium bacteria balls: Phanerochaete chrysosporium spores were added to sterile water to form a Phanerochaete chrysosporium spore suspension, and 3.5 mL of the spo...
Embodiment 2
[0042] A method for improving the antibacterial properties of nano silver, comprising the following steps:
[0043] (1) Mix the monovalent electrolyte with the nano-silver stock solution prepared in Example 1 to obtain mixed solutions with different nano-silver concentrations.
[0044] Among them, the monovalent electrolyte is NaNO 3 When the nano-silver concentration is 0μM, 10μM, 60μM, 100μM, the corresponding mixed solutions are B1, B2, B3, B4, and the NaNO 3 The concentration is 30mM.
[0045] The monovalent electrolyte is KNO 3 When the nano-silver concentration is 0μM, 10μM, 60μM, 100μM, the corresponding mixed solutions are B5, B6, B7, B8, and the KNO 3 The concentration is 30mM.
[0046] (2) get 8 parts of Phanerochaete chrysosporium bacterial balls prepared in Example 1, join respectively in the mixed solution B1~B8 that makes in the step (1), wherein the Phanerochaete chrysosporium bacterial ball The addition amount is 0.9g / L (that is, the ratio of Phanerochaete...
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