A high-efficiency nitrogen-reducing Pseudomonas monsonii strain cy06 and its microecological preparation and application
A technology of microecological preparations and pseudomonas strains, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve problems such as different action conditions and large differences in nitrite efficiency, and achieve good denitrification activity.
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Embodiment 1
[0025] Embodiment 1, the screening of CY06 bacterial strain
[0026] 1. Screen for strains with blue halos and no hemolytic circles
[0027] Screening samples were collected from 100 water samples, fish samples, shrimp samples from fish ponds and shrimp ponds in Jiangsu, Guangdong and other places, as well as seawater samples and sand samples from Qingdao. In addition, the collected fish and shrimp samples were first sterilized with alcohol cotton, and the liver and intestines were dissected, ground in a small amount of sterile water, diluted and spread on a BTB solid culture plate, and placed in a 30°C incubator for cultivation At 20 hours, pick the colony with a blue halo around it, streak it on the LB plate, and purify twice. The single colony purified twice was planted on a fresh hemolysis plate with a toothpick, placed in a 30°C incubator and observed for 24h, 48h, and 72h to see if hemolysis circles appeared. Classified according to the ability to hemolyze blood cell a...
Embodiment 2
[0053] Embodiment 2, the research of denitrification type
[0054] 1. Research on the nitrification characteristics of strain CY06
[0055] In the ultra-clean bench, the bacterial solution of the strain CY06 to be tested was inserted into 50ml of nitrification medium, and the final inoculation concentration was 5×10 5 cfu / mL, repeated 3 times; cultured in a shaker at 30°C, 180rpm, samples were taken to determine the content of ammonia nitrogen, nitrite and nitrate in the medium.
[0056] The result is as figure 1 As shown, the CY06 strain can rapidly remove NH 4 + , NH at 20h 4 + -N removal rate was 82.3%, and nitrite nitrogen was generated during the process.
[0057] 2. Study on denitrification characteristics of strain CY06
[0058] In the ultra-clean bench, the bacterial solution of the strain CY06 to be tested was inserted into 50ml denitrification medium, and the final inoculation concentration was 5×10 5 CFU / mL, repeated 3 times; cultured in a shaker at 30°C, 18...
Embodiment 3
[0063] Example 3, Whole Genome Sequencing Analysis of CY06 Strain
[0064] After whole genome sequencing analysis, it was found that the strain contained a 5,689,878bp chromosomal group, of which the G+C content was 61%, and contained 5110 coding genes; gene function analysis found that there was a nitrification and denitrification function in this strain Related enzymes, such as nitrate reductase, nitrite reductase, nitric oxide reductase, etc., and 20 enzymes related to nitrogen metabolism, such as glutamine synthetase, glutamate synthetase, nitrate monooxygenase, etc. protein.
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