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A preparation method of mesoporous bioglass/sodium alginate-sodium alginate layered bone tissue engineering scaffold

A technology of bone tissue engineering and sodium alginate, which is applied in the field of materials science, can solve the problems of fast drug release, low mechanical strength, and poor osteoconductivity, and achieve the effects of promoting proliferation and differentiation, excellent biological activity, and remarkable technological progress

Active Publication Date: 2021-11-19
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems in the prior art, the present invention provides a method for preparing a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold. The preparation of simple sodium alginate stents in the prior art has problems such as low mechanical strength, poor bone conduction, and fast drug release.

Method used

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  • A preparation method of mesoporous bioglass/sodium alginate-sodium alginate layered bone tissue engineering scaffold
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  • A preparation method of mesoporous bioglass/sodium alginate-sodium alginate layered bone tissue engineering scaffold

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Embodiment 1

[0028] A preparation method of mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold, comprising the following steps:

[0029] Step 1, preparation of mesoporous bioglass powder: a bioactive glass block with good mesoporous channels (molar ratio SiO 2 :CaO:P 2 o 5 =80:15:5), then the mesoporous bioglass block was ball milled and passed through a 400-mesh sieve, the particle size was less than 37 microns, and dried and stored for future use.

[0030] Step 2, preparation of sodium alginate layer ink: dissolving sodium alginate in deionized water (mass fraction: 25%), and magnetic stirring to obtain uniformly mixed sodium alginate ink for subsequent three-dimensional printing.

[0031]Step 3, prepare mesoporous bioglass / sodium alginate layer composite ink: Dissolve sodium alginate in deionized water to obtain a solution with a mass fraction of 10%, and add the mesoporous bioglass powder obtained in step 1 to 10% Mix well in the sodium algi...

Embodiment 2

[0036] Example 2 drug loading and release example

[0037] A method for preparing a drug-loaded mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold, comprising the following steps:

[0038] Step 1, prepare sodium alginate layer ink loaded with bovine serum albumin (BSA): dissolve BSA in deionized water to form a uniform solution, then dissolve sodium alginate in it to obtain sodium alginate layer ink loaded with BSA, The mass fraction of sodium alginate is 25%, and the mass fraction of BSA is 2%.

[0039] Step 2, preparation of mesoporous bioglass / sodium alginate layer composite ink loaded with ibuprofen drug (IBU): dissolve IBU in a small amount of absolute ethanol, and then add deionized water to make the concentration 50 mg / ml. Next, 2.0 grams of mesoporous bioglass powder was added to 100 ml of IBU solution and stirred at room temperature for 24 hours, then centrifuged, and the upper layer was taken to test its absorbance, and the ...

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Abstract

The invention provides a method for preparing a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold, comprising a step of preparing sodium alginate ink; a step of preparing mesoporous bioglass / sodium alginate composite ink steps; a step of designing the appearance and internal structure of mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold; a step of preparing mesoporous bioglass / sodium alginate-sodium alginate fraction Steps for layered bone tissue engineering scaffolds. The results of cell experiments show that the mesoporous biological glass / sodium alginate-sodium alginate layered scaffold prepared by the method of the present invention has excellent ability to promote the proliferation and differentiation of human bone marrow mesenchymal stem cells hBMSCs, and shows excellent biological activity .

Description

technical field [0001] The invention belongs to the field of materials science, and relates to a bone tissue engineering scaffold, in particular to a method for preparing a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold. Background technique [0002] At present, bone tissue defects caused by tumor resection, congenital diseases, infection and trauma are common clinical diseases. The application of bone tissue engineering scaffolds to repair the defect is considered to be one of the effective methods to solve related problems. Since bone grafting can easily cause a series of infection, inflammation and pain problems, it is difficult for simple scaffold materials to meet the requirements of ideal bone repair materials. If the drug can be directly loaded in the implant material and released in situ and locally after implantation, it can not only prevent the transfer and spread of diseased cells through the in situ release of the dr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/10A61L27/22A61L27/50A61L27/54A61L27/56B33Y10/00B33Y70/10
CPCA61L27/10A61L27/20A61L27/227A61L27/50A61L27/54A61L27/56A61L2300/252A61L2300/402A61L2300/404A61L2300/41A61L2300/412A61L2300/602A61L2430/02B33Y10/00B33Y70/00C08L5/04C08L89/00
Inventor 朱钰方杜晓宇胡亚萍
Owner UNIV OF SHANGHAI FOR SCI & TECH