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Method for extracting black rice bran anthocyanin with circulating ultrasonic

A technology of cyclic ultrasound and extraction methods, which is applied in the biological field, can solve the problems of difficult engineering amplification of extraction equipment, and achieve the effects of convenient recycling and reuse, high yield and high production efficiency

Active Publication Date: 2018-12-07
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method for extracting anthocyanins from black rice does not involve cyclic ultrasonic-assisted extraction. The present invention adopts a method of mechanical agitation and cyclic ultrasonic extraction to strengthen the crushing of plant cells, improve extraction efficiency, increase the content of anthocyanins in the extract, and solve the problem at the same time. There is a problem that the extraction equipment is difficult to scale up, and it provides a suitable method for the industrial production of anthocyanins from black rice bran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for cyclic ultrasonic extraction of anthocyanins from black rice bran includes the following steps: clean the black rice bran, dry it at a constant temperature of 50°C, and crush it to 60 mesh; and then according to the liquid-to-material ratio 1:10 (mass: volume) ) Was added 40% ethanol for cyclic ultrasonic extraction. The extraction conditions were: temperature 40°C, ultrasonic power 800W, extraction time 90min; after the extraction, centrifuge at 3000r / min for 20min to remove residue to obtain the anthocyanin extract. The liquid is concentrated under reduced pressure and spray-dried to obtain black rice bran anthocyanin powder.

Embodiment 2

[0047] A method for cyclic ultrasonic extraction of anthocyanins from black rice bran includes the following steps: washing the black rice bran, drying at a constant temperature of 50°C, and crushing it to 60 mesh; and then according to the liquid-to-material ratio 1:5 (mass: volume) ) Was added 40% ethanol for cyclic ultrasonic extraction. The extraction conditions were: temperature 40℃, ultrasonic power 1000W, extraction time 60min; after the extraction, centrifuge at 3000r / min for 20min to remove residue to obtain anthocyanin extract, and extract The liquid is concentrated under reduced pressure and spray-dried to obtain black rice bran anthocyanin powder.

Embodiment 3

[0049] A method for cyclic ultrasonic extraction of anthocyanins from black rice bran includes the following steps: washing the black rice bran, drying at a constant temperature of 50°C, and crushing it to 60 mesh; and then according to the liquid-to-material ratio 1:5 (mass: volume) ) Was added 40% ethanol for cyclic ultrasonic extraction. The extraction conditions were: temperature 40°C, ultrasonic power 1500W, extraction time 30min; after the extraction, centrifuge for 20min at 3000r / min to remove residue to obtain anthocyanin extract. The liquid is concentrated under reduced pressure and spray-dried to obtain black rice bran anthocyanin powder.

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Abstract

The invention discloses a method for extracting black rice bran anthocyanin with circulating ultrasonic. The method comprises the following steps: 1, grinding black rice bran into black rice bran powder; 2, extracting the black rice bran powder by utilizing a circulating ultrasonic extraction method, and collecting an extracting solution; and 3, drying the extracting solution to obtain the black rice bran anthocyanin. The method for extracting black rice bran anthocyanin with circulating ultrasonic has simple process, high production efficiency and high anthocyanin yield, adopts ethyl alcoholas an extracting solvent in the whole process to facilitate recycling, does not have waste water or waste gas generated in the production process, and is suitable for industrial production.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to a method for cyclic ultrasonic extraction of black rice bran anthocyanins. Background technique [0002] Black rice is the brown rice after the black rice has been husked. The surface is black or dark brown, and belongs to the japonica rice category. Black rice not only contains protein, carbohydrates, B vitamins, vitamin E, calcium, phosphorus, potassium, magnesium, iron, zinc and other rich nutrients, but also contains vitamin C, chlorophyll, anthocyanins, carotene and cardiac glycosides, etc. Special ingredients. Black rice is a kind of grain with the same medicine and food and high nutritional value. It is known as "black pearl" and "king of rice in the world". Modern pharmacological studies have shown that black rice has better effects of nourishing yin and kidney, invigorating the spleen, warming the liver, improving eyesight and promoting blood circulation. It is beneficial to the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 王凤忠杨颖刘清霞范蓓王艳王东晖张丽静幸岑璨李淑英方芳
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI