A kind of synthesis method of strong acid agar-based chromatography medium with high adsorption capacity

A synthesis method and high adsorption technology, applied in chemical instruments and methods, ion exchange, microcapsule preparations, etc., can solve the problems of slow flow rate, uneven distribution of active groups and ligands, limited pressure, etc., and achieve the degree of functionalization. High, save the preparation cost, simplify the effect of the preparation process
CN108948385BActive Publication Date: 2020-09-22AMICOGEN CHINA BIOPHARM CO LTD
9 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AMICOGEN CHINA BIOPHARM CO LTD
Publication Date
2020-09-22

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the field of biological separation of biological engineering, cells and cell pharmaceutical carriers and particularly relates to a method for synthesizing a high adsorption strong acid agar-based chromatography medium. An agarose solution is used as a water phase. Methylbenzene is an oil phase. Sodium 3-chloro-2-hydroxypropanesulfonate and epichlorohydrin realizes a functionalized and crosslinking reaction for forming agarose microspheres. The strong acid functionalization, a crosslinking reaction and a ball formation process are integrated. Finally, the balls are post-treated so that products are obtained. The method has simple processes and saves the preparation cost. The high adsorption strong acid agar-based chromatography medium has good mechanical strength, uniform distribution of functional groups and a high adsorption amount.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biological separation of biological engineering, cells and drug carriers thereof, in particular to a method for synthesizing a strong-acid agar-based chromatographic medium with high adsorption capacity. Background technique

[0002] In recent years, the upstream cell culture and expression technology has been continuously matured, and the production scale has continued to expand. The rapid development of biotechnology has brought enormous pressure to the downstream separation and purification, requiring the downstream to have greater processing capacity and higher production efficiency.

[0003] Ion-exchange chromatography (Ion-exchange chromatography, IEC) uses the ion-exchange medium as the stationary phase, and realizes the separation of solutes according to the difference in the strength of the electrostatic interaction between the charged solute in the mobile phase and the charged group of the medium. Due to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More