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Method for extracting polypeptide active substances from konjac

An active substance, konjac technology, applied in the field of extracting polypeptide active substances, can solve the problems of potential safety hazards and low biological activity of konjac polypeptide, and achieve the effects of lowering blood sugar, simple and feasible preparation method, and improving yield.

Inactive Publication Date: 2018-12-07
金华市景和科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method makes the biological activity of the obtained konjac polypeptide lower, and the konjac polypeptide extract contains 0.7% ammonium salt, which has certain potential safety hazards to the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for extracting polypeptide active substances from konjac, extracting konjac fly powder alkali to obtain konjac protein extract, then adding protease enzymolysis to the konjac protein extract to obtain enzymatic hydrolysis, and separating and purifying the enzymatic hydrolysis to obtain konjac polypeptide active substances. The NaOH solution used for alkali extraction contains ephedrine. The preparation method uses konjac flying powder, which is the waste of the usual konjac fine powder processing process, to prepare the konjac polypeptide extract with great pharmaceutical value, which has very important production significance and economic value. The preparation method is simple and feasible, and the process is stable. , high reproducibility, the equipment used is mature equipment, the investment cost is low, and the recovery rate of peptide active substances is high.

[0020] The method for extracting polypeptide active substance from konjac, its specific st...

Embodiment 2

[0028] The method for extracting polypeptide active substance from konjac, its specific steps are:

[0029] 1) Alkali extraction: add konjac flying powder to NaOH solution with a concentration of 1.5% according to the ratio of material to liquid 1:33 (g / mL), extract with alkali for 1.5 hours, and then centrifuge. The supernatant is the konjac protein extract. The NaOH solution contains 0.38mM ephedrine, and the ratio of the L-isomer and the D-isomer in the ephedrine is 100:2.5;

[0030] 2) Protease hydrolysis: preheat konjac protein extract to 65°C, adjust its pH to 6.5, add papain for constant temperature enzymolysis for 2.5 hours, cool down to 37°C, adjust its pH to 8.5, add elastase for constant temperature Enzymolysis for 2 hours, after the hydrolysis, inactivate the enzyme in a water bath at 98°C for 12 minutes, let the hydrolyzate stand to cool, and centrifuge at 8000r / min for 28min, the supernatant is the enzymolysis solution;

[0031] 3) Separation and purification: t...

Embodiment 3

[0033] The method for extracting polypeptide active substances from konjac, the further optimization steps are:

[0034] 1) Alkali extraction: add konjac flying powder to NaOH solution with a concentration of 1.5% according to the ratio of material to liquid 1:33 (g / mL), extract with alkali for 1.5 hours, and then centrifuge. The supernatant is the konjac protein extract. The NaOH solution contains 0.38mM ephedrine, and the ratio of the L-isomer and the D-isomer in the ephedrine is 100:2.5;

[0035] 2) Protease enzymatic hydrolysis: Preheat the konjac protein extract to 65°C, adjust its pH to 6.5 with phosphate buffer, add papain for constant temperature enzymolysis for 2.5 hours, and adjust its pH to 8.5 after cooling down to 37°C. Add elastase for constant temperature enzymolysis for 2 hours. After the hydrolysis, inactivate the enzyme in a water bath at 98°C for 12 minutes, let the hydrolyzate stand for cooling, and centrifuge at 8000r / min for 28min. The supernatant is the ...

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Abstract

The invention discloses a method for extracting polypeptide active substances from konjac. The method concretely comprises the following steps: carrying out alkali extraction on konjac fly powder by using a NaOH solution containing ephedrine to obtain a konjac protein extract, adding a protease to the konjac protein extract, carrying out enzymatic hydrolysis to obtain an enzymatic hydrolysate, andseparating and purifying the enzymatic hydrolysate to obtain the konjac polypeptide active substances. The prepared polypeptide active substance can be used to prepare various preparations for treating diabetes and complications thereof. The preparation method of the polypeptide active substances has the advantages of simplicity, feasibility, low cost, stable process, removal of a large amount ofsalts, and high yield; and the obtained product has the advantages of high purity and high quality.

Description

technical field [0001] The invention relates to the technical field of bioactive component extraction, in particular to a method for extracting polypeptide active substances from konjac. Background technique [0002] Konjac ( Amorphophallus rivieri ) is a perennial herbaceous tuber plant belonging to the Araceae family Anophyllum. It is monoecious and likes warm, humid and semi-shaded ecological environments. There are 163 varieties in the world. There are 21 konjac varieties in my country, most of which grow in Sichuan, Shaanxi, Ningxia, Jiangsu, Guizhou, Hunan, Yunnan and other places. The ingredients of konjac are mainly derived from the tuber. Its tubers contain about 70% of glucomannan and starch, about 5%~15% of protein and amino acid, about 4%~6% of various minerals, and 1%~2% of alkaloids and other ingredients. . Konjac has a wide range of edible value, medicinal value, industrial value and application value in other fields. Under such conditions, the future res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34A61K38/01A61P3/10
CPCA61K38/011A61P3/10C07K1/145C07K1/34C12P21/06
Inventor 汪珂
Owner 金华市景和科技有限公司
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