Functional marker of maize recessive nuclear male sterility mutant gene ms1 and applications thereof

Abstract

The invention relates to a functional marker of a maize nuclear male sterility gene ms1, a functional marker developing method and applications of the functional marker. According to the mutation siteof the mutant gene ms1, the functional marker which comprises two functional markers ms1-IN1 and ms1-IN2 is designed to ensure that a maize mutant ms1-alb and the mutant gene ms1 in a maize sterile material transferred from mutant can be specifically detected; and by utilizing the ms1-IN1, the ms1-IN2 or other similar functional markers and functional markers Ms1-1, Ms1-2 or other similar functional markers of a corresponding wild type allele Ms1, the situation of a nuclear male sterility gene Ms1 allele in a sample can be quickly identified through PCR (Polymerase Chain Reaction) detection and agarose gel electrophoresis detection. By utilizing the functional marker designed by the invention, the allele genotypes of the maize nuclear male sterility gene ms1 or a fertile gene Ms1 can be accurately detected and marked; the functional marker can be used for identification of male sterile strains and screening of target single stains; and the functional marker has important application value in maize male sterile strain breeding, sterile hybrid seed production and molecular marker-assisted selection and has important significance for improving maize cross breeding efficiency and seedproduction efficiency, guaranteeing seed production quality and the like.

Description

technical field [0001] Generally, the present invention is in the fields of crop molecular breeding, molecular biology and genetic engineering. Concretely relates to a kind of maize recessive male sterility gene ms1 Functional flags, functional flag development methods and applications. Background technique [0002] Plant male sterility (male sterility, MS) refers to the abnormal development of male organs in higher plants, unable to produce functional male gametes (pollen), but the normal development of female organs, which can receive normal male gametes for fertilization and fruiting, and can The phenomenon in which sterility is passed on to offspring. [0003] Maize is an important food crop, and its male sterile materials are of great value in the study of maize male flower development mechanism, genetic breeding application, heterosis research and application. Maize male sterility can be divided into three types according to the differences in sterility inheritance ...

AI Technical Summary

Problems solved by technology

Therefore, since the discovery of maize recessive genetic sterile materials, people have used traditional breeding techniques to explore and study various marker traits of the genetic sterile gene, such as using the close linkage relationship between marker traits and sterility, The grain color marker system method, the yellow-green seedling linkage marker method, and the multi-filament linkage marker system have been developed. However, due to the incomplete linkage between marker traits and sterility, difficulties in the identification of marker traits, and delays in the identification period, these methods and attempts have been in the field. It has not been widely used in corn production

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